8,404 results
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Plasmid#129659PurposeHPHex_CrisprCas9-yl_RFP: CAS9 vector with Hygromycinex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only
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pCS4333 yeTadA1.0-T7 RNAP
Plasmid#137735PurposepCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable markerDepositorInsertyeTadA1.0-T7 RNAP
ExpressionBacterial and YeastMutationE366G (See depositor comment below)Available SinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
bRA89
Plasmid#100950PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
Plasmid#83946PurposeCas9-carrying vector with kanMX markerDepositorInsertCas9
ExpressionYeastAvailable SinceOct. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natMX-(3×FLAG)-TEV-ProtA
Plasmid#52693PurposeFor C-terminal tagging of ORFs with a (3×FLAG)-TEV-ProtADepositorInsert(3×FLAG)-TEV-ProtA
ExpressionYeastAvailable SinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G1-Ph
Plasmid#46814PurposeTEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformationDepositorTypeEmpty backboneTagsFLAG and c-mycExpressionYeastPromoterTEF1 and PGK1Available SinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pE4c
Plasmid#17464Purposemodified pENTR vector for C-terminal GST tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsGSTExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pNKY51
Plasmid#14839DepositorInserthisG-URA3-hisG
ExpressionYeastAvailable SinceApril 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBHM2403
Plasmid#171687PurposepRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.DepositorInsertCnoCAS9
UseCRISPRExpressionBacterial and YeastPromoterTEF1Available SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR_D17_hrGFP
Plasmid#84616PurposeHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into D17 locusDepositorInserthrGFP
UseSynthetic BiologyExpressionYeastPromoterUAS1B8-TEF(136)Available SinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
Plasmid#78231PurposeExpresses Cas9 protein, under the control of the TEF1 promoter, and CYC terminator. KanMX resistanceDepositorInsertCas9
ExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
YIP sHRPa-Aga1P
Plasmid#73151PurposeLarge fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.DepositorInsertsHRPa-Aga1P
TagsV5 tagExpressionYeastPromoterglyceraldehyde-3-phosphate dehydrogenase GPD prom…Available SinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS416-yZ3EV-Z3pr-yEGFP (RB3579)
Plasmid#69100PurposeURA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFPDepositorInsertsyZ3EV cassette
Z3 promoter
yEGFP
ExpressionYeastAvailable SinceSept. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G2-Km
Plasmid#46815PurposeTEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneTagsFLAG and c-mycExpressionYeastPromoterTEF1 and PGK1Available SinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRS314-4M5.3
Plasmid#45830DepositorInsert4M5.3
TagsFLAGExpressionYeastPromoterGAL1Available SinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoGFPgamma-SpHis5
Plasmid#44859PurposeYeast tagging vector to create gene fusion with yeast optimized GFPgamma with SpHis5 selectionDepositorInsertyoGFPgamma
ExpressionYeastAvailable SinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGPD2
Plasmid#43972DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastPromoterGPDAvailable SinceApril 9, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
YIPlac204TC-NLS-DsRed-Express2
Plasmid#25443DepositorInsertNLS-DsRed-Express2
TagsNLSExpressionYeastAvailable SinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHS12-mCherry
Plasmid#25444DepositorInsertmCherry
TagsCOX4 PresequenceExpressionYeastAvailable SinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -