8,085 results

1. pJK-caCas9-NatMX-Neut5L-Ade2 drive

   Plasmid

   #89577

   Purpose

   caCas9 integrating vector into the Neut5L locus with gene drive for targeting Ade2 locus

   Depositor

   George Church

   Article

   Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

   Insert

   Ade2 gene drive
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCLASP_mScarlet_CLASP_RGS2membrane

   Plasmid

   #133086

   Purpose

   Plasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to bl

   Depositor

   Hana El-Samad

   Article

   Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

   Insert

   mScarlet-CLASP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK-caCas9-NatMX-Neut5L-Leu2 drive

   Plasmid

   #89578

   Purpose

   caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus

   Depositor

   George Church

   Article

   Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

   Insert

   Leu2 gene drive
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGBKCg

   Plasmid

   #20162

   Depositor

   Peter Uetz

   Article

   Stellberger et al Proteome Sci. 2010 Feb 15;8:8. doi: 10.1

   Type

   Empty backbone

   Use

   Tags

   Gal4 DBD

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. YIplac211-KAR2-msGFP2-HDEL

   Plasmid

   #160468

   Purpose

   Yeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to msGFP2 with an ER retrieval signal

   Depositor

   Benjamin Glick

   Article

   Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

   Insert

   KAR2-msGFP2-HDEL
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-6xGLY-Myc-hphMX4

   Plasmid

   #20770

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA6a-6xGLY-FLAG-hphMX4

   Plasmid

   #20752

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p415 TEF roGFP2‐Tsa2ΔCR

   Plasmid

   #83238

   Purpose

   The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

   Depositor

   Tobias Dick

   Article

   Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

   Insert

   roGFP2-Tsa2dCr
   

   Use

   Tags

   roGFP2 fusion with Tsa2dCr

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available Since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-ATG8(416)/GFP-AUT7(416)

   Plasmid

   #49425

   Purpose

   Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

   Depositor

   Daniel Klionsky

   Article

   Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

   Insert

   autophagy-related 8 (ATG8 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   ATG8

   Available Since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. YIplac128-pOst1-ESCargo

    Plasmid

    #115421

    Purpose

    Expresses a regulatable secretory cargo for yeast at the LEU2 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

    Insert

    pOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. LifeAct-mScarletI pDRF1-GW

    Plasmid

    #168085

    Purpose

    Expresses Lifeact-mScarletI to visualize actin in yeast

    Depositor

    Bas Teusink

    Insert

    LifeAct-mScarletI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTDH3-dCas9-Mxi1

    Plasmid

    #46921

    Purpose

    Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoter

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    dCas9-Mxi1
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGR481

    Plasmid

    #213783

    Purpose

    Integrates onto p1 a catalytically dead TRP5 and URA3 that can be used for selection of transversion mutations

    Depositor

    Chang Liu

    Insert

    ura3*
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RCP83

    Plasmid

    #163466

    Purpose

    Backbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFP

    Depositor

    Frank Albert

    Article

    Renganaath et al Elife. 2020 Nov 12;9. pii: 62669. doi: 1

    Inserts

    Promoter fragment (Sharon2012_1922)
    His3 minimal promoter
    yeGFP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIU211

    Plasmid

    #44036

    Depositor

    Dane Wittrup

    Article

    Boder et al Nat Biotechnol. 1997 Jun;15(6):553-7.

    Insert

    AGA1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. HO-hisG-URA3-hisG-poly-HO

    Plasmid

    #51663

    Purpose

    yeast plasmid for integration of target sequence at HO locus, URA3 selection

    Depositor

    David Stillman

    Article

    Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

    Type

    Empty backbone

    Use

    Yeast integration

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDB5320

    Plasmid

    #204830

    Purpose

    An integrating plasmid containing both the inducible enotetSW2 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

    Depositor

    Li-Lin Du

    Article

    Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

    Inserts

    tetR
    mECitrine
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CMV promoter and enotetSW2 promoter

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. FRP1642_PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

    Plasmid

    #58442

    Purpose

    Cassette to replace yeast promoters with an inducible promoter containing 4 lexA boxes

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETcon(-)

    Plasmid

    #41522

    Depositor

    Andrew Scharenberg

    Type

    Empty backbone

    Use

    Tags

    3x(G4S) Linker, G3S Linker, HA, Myc Tag, and Stuf…

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGADCg

    Plasmid

    #20161

    Depositor

    Peter Uetz

    Article

    Stellberger et al Proteome Sci. 2010 Feb 15;8:8. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    Gal4 AD and SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge