8,418 results

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NM9-SpCas9-NLS3

Plasmid

#128177

Purpose

Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

Depositor

Huimin Zhao

Publication

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

SpCas9-NLS3

Use

CRISPR

Expression

Yeast

Promoter

pPGK1

Available since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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JME4580

Plasmid

#129659

Purpose

HPHex_CrisprCas9-yl_RFP: CAS9 vector with Hygromycinex marker for gRNA cloning using GoldenGate

Depositor

Tristan Rossignol

Publication

Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCS4333 yeTadA1.0-T7 RNAP

Plasmid

#137735

Purpose

pCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable marker

Depositor

Christina Smolke

Publication

Cravens et al Nat Commun. 2021 Mar 11;12(1):1579. doi:

Insert

yeTadA1.0-T7 RNAP

Expression

Bacterial and Yeast

Mutation

E366G (See depositor comment below)

Available since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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bRA89

Plasmid

#100950

Purpose

Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH marker

Depositor

James Haber

Publication

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

Plasmid

#83946

Purpose

Cas9-carrying vector with kanMX marker

Depositor

Irina Borodina

Publication

Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

Insert

Cas9

Expression

Yeast

Available since

Oct. 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pFA6a-natMX-(3×FLAG)-TEV-ProtA

Plasmid

#52693

Purpose

For C-terminal tagging of ORFs with a (3×FLAG)-TEV-ProtA

Depositor

Michael Nick Boddy

Insert

(3×FLAG)-TEV-ProtA

Expression

Yeast

Available since

May 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCEV-G1-Ph

Plasmid

#46814

Purpose

TEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Publication

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and PGK1

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pFA6a-link-yoGFPgamma-SpHis5

Plasmid

#44859

Purpose

Yeast tagging vector to create gene fusion with yeast optimized GFPgamma with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoGFPgamma

Expression

Yeast

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGPD2

Plasmid

#43972

Depositor

Hal Alper

Publication

Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Promoter

GPD

Available since

April 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

6 Articles

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pE4c

Plasmid

#17464

Purpose

modified pENTR vector for C-terminal GST tag fusion with your gene of interest

Depositor

Giovanna Benvenuto

Publication

Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

Type

Empty backbone

Use

Modified gateway entry vector

Tags

GST

Expression

Bacterial, Insect, Mammalia…

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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pNKY51

Plasmid

#14839

Depositor

Nancy Kleckner

Publication

Alani et al Genetics. 1987 Aug . 116(4):541-5.

Insert

hisG-URA3-hisG

Expression

Yeast

Available since

April 27, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pRS428

Plasmid

#11257

Depositor

Fred Cross

Type

Empty backbone

Expression

Yeast

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pBHM2403

Plasmid

#171687

Purpose

pRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.

Depositor

Hiten Madhani

Publication

Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

Insert

CnoCAS9

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

TEF1

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFA6a-link-ymNeongreen-URA3

Plasmid

#168059

Purpose

Replaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FP

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeonGreen

Tags

ymNeonGreen

Expression

Yeast

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pYES1L-URA

Plasmid

#84301

Purpose

E.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.

Depositor

George Church

Publication

Ostrov et al Science. 2016 Aug 19;353(6301):819-22. d

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHR_D17_hrGFP

Plasmid

#84616

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into D17 locus

Depositor

Ian Wheeldon

Publication

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

Plasmid

#78231

Purpose

Expresses Cas9 protein, under the control of the TEF1 promoter, and CYC terminator. KanMX resistance

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

Cas9

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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YIP sHRPa-Aga1P

Plasmid

#73151

Purpose

Large fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.

Depositor

Alice Ting

Publication

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPa-Aga1P

Tags

V5 tag

Expression

Yeast

Promoter

glyceraldehyde-3-phosphate dehydrogenase GPD prom…

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRS416-yZ3EV-Z3pr-yEGFP (RB3579)

Plasmid

#69100

Purpose

URA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFP

Depositor

David Botstein

Inserts

yZ3EV cassette
Z3 promoter
yEGFP

Expression

Yeast

Available since

Sept. 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCEV-G2-Km

Plasmid

#46815

Purpose

TEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Publication

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and PGK1

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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