8,085 results

1. pNX32-Dest

   Plasmid

   #105083

   Purpose

   Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

   Depositor

   Christopher Grefen

   Article

   Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

   Type

   Empty backbone

   Use

   Tags

   NubG

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNTI647 dCas9-Mxi1 TetR KanMX

   Plasmid

   #139474

   Purpose

   Integrates CRISPRi effector and tet-responsive regulator

   Depositor

   Nicholas Ingolia

   Article

   McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

   Inserts

   dCas9-Mxi1
   Tet Repressor
   

   Use

   CRISPR

   Tags

   Mxi1 and NLS

   Expression

   Yeast

   Mutation

   D10A, H840A

   Promoter

   pGPM1 and pTEF1

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGR475

   Plasmid

   #213784

   Purpose

   Integrates at the CAN1 locus the WT TP-DNAP1 and the Hygromycin resistance marker

   Depositor

   Chang Liu

   Insert

   WT TP-DNAP1 (recoded)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-link-ymNeongreen-SpHis5

   Plasmid

   #125704

   Purpose

   Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with SpHIS5 selection

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymNeonGreen
   

   Use

   Tags

   ymNeonGreen

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSC-humanADAR1

   Plasmid

   #198235

   Purpose

   Expresses human ADAR1 in yeast

   Depositor

   Brenda Bass

   Article

   Eggington et al Nat Commun. 2011;2:319. doi: 10.1038/nco

   Insert

   human ADAR1 (ADAR Human)
   

   Use

   Tags

   His10_TEV

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCT302

   Plasmid

   #41845

   Depositor

   Dane Wittrup

   Article

   Midelfort et al J Mol Biol. 2004 Oct 22;343(3):685-701.

   Insert

   4-4-20
   

   Use

   Tags

   HA and c-myc

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pG3

   Plasmid

   #162604

   Purpose

   Edit Ade2 gene in yeast

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG303-Gal-PA50

   Plasmid

   #84906

   Purpose

   induce expression of dipeptide repeat PA in yeast

   Depositor

   Aaron Gitler

   Article

   Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

   Insert

   PA50 (C9orf72 Synthetic)
   

   Use

   Tags

   FLAG and Myc

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   April 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pABY-c15

   Plasmid

   #201768

   Purpose

   Expresses mNeonGreen-tagged NbALFA under ADH1 promoter in yeast

   Depositor

   Saravanan Palani

   Article

   Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

   Insert

   NbALFA
   

   Use

   Tags

   40aa Linker, mNeonGreen

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-link-ytdTomato-URA3

    Plasmid

    #168054

    Purpose

    Replaces Addgene plasmid 118461. Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ytdTomato
    

    Use

    Tags

    ytdTomato

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDB5321

    Plasmid

    #204831

    Purpose

    An integrating plasmid containing both the inducible enotetSW3 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

    Depositor

    Li-Lin Du

    Article

    Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

    Inserts

    tetR
    mECitrine
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CMV promoter and enotetSW3 promoter

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. YIP sHRPa-Aga1P

    Plasmid

    #73151

    Purpose

    Large fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    sHRPa-Aga1P
    

    Use

    Tags

    V5 tag

    Expression

    Yeast

    Mutation

    Promoter

    glyceraldehyde-3-phosphate dehydrogenase GPD prom…

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYDS2.0_AT118-H

    Plasmid

    #222846

    Purpose

    Yeast-display plasmid encoding Nb. AT118-H, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-H
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. TetR in pENTR1A (559-1)

    Plasmid

    #22265

    Depositor

    Eric Campeau

    Insert

    Tetracycline repressor (tetR )
    

    Use

    Tags

    Expression

    Insect, Mammalian, Worm Exp…

    Mutation

    Promoter

    Available Since

    Dec. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. SIVu

    Plasmid

    #81089

    Purpose

    Single Integration Vector URA - Cloning vector for single integration into the URA3 locus of S.cerevisiae containing two MCS.

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETcon_SARS-CoV-2_RBD

    Plasmid

    #166782

    Purpose

    Yeast surface display vector, expressing the SARS-2 RBD, 201aa construct

    Depositor

    Jesse Bloom

    Article

    Starr et al Cell. 2020 Sep 3;182(5):1295-1310.e20. d

    Insert

    Spike receptor binding domain (RBD) from SARS-CoV-2 (S )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS327

    Plasmid

    #51787

    Purpose

    multicopy (YEp) vector with a LYS2 marker and a 2-μm origin of replication

    Depositor

    David Stillman

    Article

    Eriksson et al Biotechniques. 2004 Feb;36(2):212-3.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pABY-c45

    Plasmid

    #201771

    Purpose

    Expresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeast

    Depositor

    Saravanan Palani

    Article

    Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

    Insert

    NbALFA
    

    Use

    Tags

    40aa Linker, mNeonGreen

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSCupSup35GFP

    Plasmid

    #1087

    Depositor

    Susan Lindquist

    Article

    Glover et al Cell 1997 May 30;89(5):811-9.

    Insert

    SUP35 (SUP35 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 9, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAD-IRP

    Plasmid

    #220751

    Purpose

    Plasmid expresses the rabbit Iron Regulatory Protein fused to the Gal 4 Activation Domain.

    Depositor

    Marvin Wickens

    Article

    Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

    Insert

    Iron Regulatory Protein (ACO1 )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge