8,085 results

1. pAG416GPD-Cerulean-YPT7

   Plasmid

   #18847

   Depositor

   Susan Lindquist

   Article

   Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

   Insert

   YPT7 (YPT7 Budding Yeast)
   

   Use

   Tags

   Cerulean

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pRS420

   Plasmid

   #11259

   Depositor

   Fred Cross

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCASB

   Plasmid

   #190175

   Purpose

   Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence

   Depositor

   Jessica Tyler

   Article

   Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:

   Insert

   sgRNA cloning site
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Added 5'-agagacc-3' upstream and 5'…

   Promoter

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUDP025

   Plasmid

   #103874

   Purpose

   Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces lactis

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Insert

   HH-gRNA-HDV targetting ADE2 in Kluyveromyces lactis
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. YIp LYS2-LEU2

   Plasmid

   #122909

   Purpose

   LYS2-LEU2 integrating (YIp) vector for stable genomic integration in the BY4741 background.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG416GPD-Cerulean-VPS21

   Plasmid

   #18842

   Depositor

   Susan Lindquist

   Article

   Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

   Insert

   VPS21 (VPS21 Budding Yeast)
   

   Use

   Tags

   Cerulean

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pTA-Mob 2.0 Tp

   Plasmid

   #188619

   Purpose

   Mutated pTA-Mob 2.0 Backbone in TraJ Promoter Region

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

   Type

   Empty backbone

   Use

   Synthetic Biology; Conjugation helper plasmid

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG416GPD-Cerulean-YPT31

   Plasmid

   #18849

   Depositor

   Susan Lindquist

   Article

   Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

   Insert

   YPT31 (YPT31 Budding Yeast)
   

   Use

   Tags

   Cerulean

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pPICZA_CeTRIC-B1_Del

   Plasmid

   #87344

   Purpose

   Inducible expression of CeTRIC-B1 in P.Pichia strains

   Depositor

   Zhenfeng Liu

   Article

   Yang et al Nature. 2016 Oct 27;538(7626):537-541. d

   Insert

   TRIC-B1
   

   Use

   Tags

   polyhistidine

   Expression

   Yeast

   Mutation

   Δ248-297

   Promoter

   AOX1

   Available Since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK1

    Plasmid

    #111722

    Purpose

    to express human BLM protein in yeast cells

    Depositor

    Ian Hickson

    Article

    Karow et al J Biol Chem. 1997 Dec 5;272(49):30611-4.

    Insert

    BLM (BLM Human)
    

    Use

    Tags

    hexahistidine

    Expression

    Yeast

    Mutation

    a C-terminal hexahistidine tag was added, a yeast…

    Promoter

    GAL1

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6a-link-yomRuby2-Kan

    Plasmid

    #44953

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mRuby2 with Kan selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lifeact-3xmNG::HIS3

    Plasmid

    #227596

    Purpose

    Integration vector for expressing Lifeact-3xmNeonGreen

    Depositor

    Bruce Goode

    Article

    Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

    Insert

    Lifeact-3xmNeonGreen::HIS3
    

    Use

    Tags

    3xmNeonGreen

    Expression

    Yeast

    Mutation

    Truncated ABP140 (aa 1-17 only)

    Promoter

    Abp140

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDR-GW FLIPglu-600μΔ13V

    Plasmid

    #28001

    Depositor

    Wolf Frommer

    Article

    Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

    Insert

    FLIPglu-600μΔ13V
    

    Use

    Tags

    eCFP and eYFP

    Expression

    Yeast

    Mutation

    F16A in mglB

    Promoter

    Available Since

    March 31, 2011

    Availability

    Academic Institutions and Nonprofits only

14. p2B-58

    Plasmid

    #177928

    Purpose

    Expresses yeGFP under control of doxycycline-responsive TET4 promoter (contains four rtTA binding sites)

    Depositor

    Vadim Gladyshev

    Article

    Barre et al (unpublished)

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TET4 (contains four rtTA binding sites)

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pABY-T2

    Plasmid

    #201765

    Purpose

    For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

    Depositor

    Saravanan Palani

    Article

    Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

    Insert

    ALFA tag
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDB5319

    Plasmid

    #204829

    Purpose

    An integrating plasmid containing both the inducible enotetSW1 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

    Depositor

    Li-Lin Du

    Article

    Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

    Inserts

    tetR
    mECitrine
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CMV promoter and enotetSW1 promoter

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. SIVl

    Plasmid

    #81090

    Purpose

    Single Integration Vector LEU - Cloning vector for single integration into the LEU2 locus of S.cerevisiae containing two MCS.

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRG203MX

    Plasmid

    #64521

    Purpose

    Integrative E. coli/S. cerevisiae shuttle vector

    Depositor

    Joerg Stelling

    Article

    Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYES1L-URA

    Plasmid

    #84301

    Purpose

    E.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.

    Depositor

    George Church

    Article

    Ostrov et al Science. 2016 Aug 19;353(6301):819-22. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBF3060

    Plasmid

    #26853

    Purpose

    Expression of yeast codon-optimized CreA recombinase from the GAL1 promoter

    Depositor

    Suzanne Sandmeyer, Nancy DaSilva

    Article

    Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

    Insert

    CreA recombinase
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    N/A

    Promoter

    GAL1

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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