8,173 results

1. pYTK-DN4

   Plasmid

   #180285

   Purpose

   KanR multi-gene yeast expression vector (GFP-dropout)

   Depositor

   Andreas Milias-Argeitis

   Article

   Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

   Insert

   ConLS' - GFP - ConRE' - KanR - CEN6/ARS4
   

   Expression

   Bacterial and Yeast

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMTU-DO-HIS-C4

   Plasmid

   #160340

   Purpose

   High-copy expression vector for K. marxianus, histidine selection

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Expression

   Yeast

   Promoter

   n/a

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. BB3cN_pGAP_23*_pPFK300_Cas9

   Plasmid

   #104911

   Purpose

   hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pS1643 (pFA6-NATMX-CDC42pr-yGFP1-10)

   Plasmid

   #86420

   Purpose

   N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   CDC42pr-GFP1-10
   

   Tags

   GFP1-10

   Expression

   Yeast

   Available Since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTPGI_dCas9

   Plasmid

   #64279

   Purpose

   encodes yeast-optimized dCas9 synthetic transcription factor

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   Yeast-optimized dCas9
   

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   D10A & H840A relative to S. pyogenes Cas9

   Promoter

   pTPGI

   Available Since

   May 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMTU-DO-LEU-C4

   Plasmid

   #160341

   Purpose

   High-copy expression vector for K. marxianus, leucine selection

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Expression

   Yeast

   Promoter

   n/a

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFG2

   Plasmid

   #87250

   Purpose

   gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT). Also known as pANCH1-Nat

   Depositor

   Kerstin Bystricky

   Article

   Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

   Insert

   Nat
   

   Tags

   parS segments

   Expression

   Yeast

   Promoter

   TEF

   Available Since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPT24-mtnluc

   Plasmid

   #184339

   Purpose

   Transformation of mitochondrial DNA in yeast

   Depositor

   Martin Ott

   Article

   Rzepka et al STAR Protoc. 2022 May 25;3(2):101359. do

   Insert

   nanoluciferase recoded for mitochondrial expression flanked by COX2 UTRs
   

   Expression

   Yeast

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCTCON2-Aga1p

   Plasmid

   #158141

   Purpose

   Expresses Aga1p in yeast

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   Aga1p
   

   Expression

   Yeast

   Promoter

   GAL10

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGal4BD-CRY2

    Plasmid

    #28243

    Purpose

    Expresses Gal4 DNA Binding Domain fused to CRY2 for blue-light-induced transcription activation

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Tags

    Gal4BD

    Expression

    Yeast

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. YIp204-PADH1-atTIR1-9myc

    Plasmid

    #99532

    Purpose

    S. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)

    Depositor

    Helle Ulrich

    Insert

    TIR1 (TIR1 Mustard Weed)
    

    Tags

    9xMyc

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS-SKII-3XHA-HALO-NAT

    Plasmid

    #188931

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-HALO-NAT
    

    Expression

    Yeast

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG423GAL-ccdB

    Plasmid

    #14149

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Expression

    Yeast

    Available Since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA6a-5FLAG-hphMX6

    Plasmid

    #19342

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

    Insert

    hphMX6
    

    Expression

    Yeast

    Available Since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. NM9-SpCas9-NLS3

    Plasmid

    #128177

    Purpose

    Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

    Depositor

    Huimin Zhao

    Article

    Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

    Insert

    SpCas9-NLS3
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    pPGK1

    Available Since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPRa_VPR_yl

    Plasmid

    #107677

    Purpose

    CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:

    Inserts

    Codon optimized dCas9-VPR
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSH62-EBD

    Plasmid

    #49455

    Purpose

    yeast expression of Cre recombinase fused to the estrogen binding domain (EBD), which confers estradiol-dependent control to Cre recombinase

    Depositor

    Marc Gartenberg

    Article

    Cheng et al Nucleic Acids Res. 2000 Dec 15;28(24):E1

    Insert

    Cre-EBD fusion
    

    Use

    Cre/Lox

    Expression

    Yeast

    Promoter

    Gal1 promoter

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDRF1 mScarlet3-T2A-mTurquoise2

    Plasmid

    #219848

    Purpose

    equimolar expression of mScarlet3 and mTurquoise2 in budding yeast

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2025.06.27.661975; doi: https://

    Insert

    mScarlet3-T2A-mTurquoise2
    

    Expression

    Yeast

    Available Since

    Aug. 27, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pYeDP60-hATP13A2-WT

    Plasmid

    #171377

    Purpose

    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888

    Depositor

    Peter Vangheluwe

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    human ATP13A2 variant 2 wild-type
    

    Tags

    BAD tag

    Expression

    Yeast

    Promoter

    URA3

    Available Since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-ATG8(416)/GFP-AUT7(416)

    Plasmid

    #160389

    Purpose

    Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter , NAT selection

    Depositor

    John McCusker

    Insert

    autophagy-related 8 (ATG8 Budding Yeast)
    

    Use

    Cen/ars

    Expression

    Yeast

    Promoter

    Endogenous ATG8 promoter

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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