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Plasmid#110213PurposeYeast Surface Display of G6des13DepositorInsertG6des13
UseTagscmyc tagExpressionYeastMutationL A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95FPromoterAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
VEGF binding scFv G6
Plasmid#111716PurposeYeast Surface Display of G6 as a reference starting point for designDepositorInsertG6
UseTagscmyc tagExpressionYeastMutationPromoterAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB6630
Plasmid#106160PurposeEasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6371 into Yarrowia lipolytica chromosomal location IntC_3, amp resistanceDepositorInsertunknown
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYEXNH3
Plasmid#49024PurposeFX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag and 3C protease cleavage siteDepositorTypeEmpty backboneUseTags3C protease site (PreScission site) and decaHis (…ExpressionYeastMutationPromoterGAL1Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJW1517
Plasmid#62387PurposepFA6a Osm1(M33A)-yoEGFP::LEU2DepositorInsertOSM1-EGFP fusion
UseTagsEGFPExpressionYeastMutationMethionine 33 mutated to AlaninePromoterAdh1pAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pE6n
Plasmid#17460Purposemodified pENTR vector for N-terminal EYFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsEYFPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
SIVu
Plasmid#81089PurposeSingle Integration Vector URA - Cloning vector for single integration into the URA3 locus of S.cerevisiae containing two MCS.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYZ463
Plasmid#187971PurposeTEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4tDepositorInsertCas9 (codon optimized for human)
UseSynthetic BiologyTagsSV40ExpressionYeastMutationPromoterTEF1Available sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCfB6371
Plasmid#106153PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6630, integration into Yarrowia lipolytica chromosomal location IntC_3, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoTagRFP-T-Kan
Plasmid#44906PurposeYeast tagging vector to create gene fusion with yeast optimized TagRFP-T with Kan selectionDepositorInsertyoTagRFP-T
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRS42H_PTEF1_dCas9_TCYC1
Plasmid#177155PurposeExpresses dCas9 in yeast cellsDepositorInsertNuclease-null Cas9
UseTagsSV40 NLSExpressionYeastMutationPromoterTEF1 promoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Designed VEGF-binding scFv G6des1
Plasmid#110212PurposeYeast Surface Display of G6des1DepositorInsertG6des1
UseTagscmyc tagExpressionYeastMutationL A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…PromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB4787
Plasmid#106147PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-ccdB-DsRed
Plasmid#14372DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
PP78
Plasmid#78948PurposeSupplemental Figure 3. Integration Site 5.DepositorInsertIntegration Site 5
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
ZJOM90
Plasmid#133647PurposeIntegration of SS-mCherry-HDEL in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast endoplasmic reticulum (ER).DepositorInsertpTPI1-SS-mCherry-HDEL
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymScarletI-URA3
Plasmid#168055PurposeReplaces Addgene plasmid 118457. Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymScarletI
UseTagsymScarletIExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYTK-DN5
Plasmid#180286PurposeNatR multi-gene yeast expression vector (GFP-dropout)DepositorInsertConLS' - GFP - ConRE' - NatR - CEN6/ARS4
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTPGI_dCas9_VP64
Plasmid#49013Purposeencodes yeast-optimized dCas9_VP64 synthetic transcription factorDepositorInsertdCas9_VP64 (codon-optimized for expression in S. cerevisiae)
UseCRISPR and Synthetic BiologyTagsSV40 NLSExpressionYeastMutationPromoterpTPGI (galactose+aTc inducible)Available sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-FLAG-kanMX6
Plasmid#20751DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionYeastMutationPromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only