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pDRF1-GW AKAR3-EV

Plasmid

#182533

Purpose

Expresses the PKA FRET sensor AKAR3-EV in budding yeast

Depositor

Bas Teusink

Article

Botman et al FEMS Yeast Res. 2023 Jan 4;23:foad029. d

Insert

AKAR3-EV

Expression

Yeast

Promoter

PMA1

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pNK5712

Plasmid

#219752

Purpose

pGAP-like vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - nnH3H_v2 - tAOX

Expression

Yeast

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-Kar2-Beta-Amyloid

Plasmid

#181707

Purpose

Galactose inducible expression of Kar2-Beta-Amyloid

Depositor

Alejandro Chavez

Insert

Kar2-Beta-Amyloid

Expression

Yeast

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-mTID-HA3-HIS3MX6

Plasmid

#227983

Purpose

To insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID,HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

miniTurboID

Tags

HA tag

Expression

Yeast

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-ccdB-EGFP

Plasmid

#14203

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yEmCFP-Hygro

Plasmid

#44644

Depositor

Kurt Thorn

Insert

yEmCFP

Expression

Yeast

Mutation

A206R

Available Since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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p406TDH3

Plasmid

#15977

Depositor

Nicolas Buchler, Fred Cross

Insert

TDH3 promoter + polylinker + CYC1 terminator (TDH3 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

TDH3 promoter is between SacI and XbaI restrictio…

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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LYS-Leu2

Plasmid

#218114

Purpose

Integration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar3

Plasmid

#232740

Purpose

Expresses NADPH/NADP+ biosensor NAPstar3 in S. cerevisiae.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar3

Expression

Yeast

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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Lifeact-3xmNG::URA3

Plasmid

#227595

Purpose

Integration vector for expressing Lifeact-3xmNeonGreen

Depositor

Bruce Goode

Article

Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

Insert

Lifeact-3xmNeonGreen::URA3

Tags

3xmNeonGreen

Expression

Yeast

Mutation

Truncated ABP140 (aa 1-17 only)

Promoter

Abp140

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pVS1

Plasmid

#33143

Depositor

Michael Rosbash

Article

Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

Insert

LacO (256 copies)

Expression

Yeast

Available Since

Dec. 20, 2011

Availability

Academic Institutions and Nonprofits only
pIGAhRC

Plasmid

#112510

Purpose

human Ah receptor and Arnt cDNAs expressed under control of Gal1,10 bidirectional promoter

Depositor

Charles Miller

Article

Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3

Insert

HIF1B, AhR (ARNT, AHR Human, Budding Yeast)

Use

Synthetic Biology

Expression

Yeast

Promoter

Gal1,10

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomTagBFP2-SpHis5

Plasmid

#44839

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mTagBFP2 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomTagBFP2

Expression

Yeast

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pYES2-gRNA-hyg-MCS

Plasmid

#107734

Purpose

E. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.

Depositor

Sheng Yang

Article

Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

Insert

X-3 site gRNA expression cassettes

Use

CRISPR

Expression

Yeast

Promoter

SNR52p

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pRS426GFP-2×PH(PLCδ)

Plasmid

#36092

Depositor

Scott Emr

Article

Stefan et al Mol Biol Cell. 2002 Feb;13(2):542-57.

Insert

2xPH(PLCdelta) fusion

Tags

GFP

Expression

Yeast

Mutation

contains aa11-140 of PLCδ (including the PH domai…

Promoter

PRC1

Available Since

June 14, 2012

Availability

Academic Institutions and Nonprofits only

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pNK5867

Plasmid

#219751

Purpose

pGAP-like vector encoding hispidin-synthase from Mycena citricolor under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - mcitHispS - tAOX

Expression

Yeast

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pNK1508

Plasmid

#219750

Purpose

pGAP-like vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - npgA - tAOX

Expression

Yeast

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMTU-DO-G418-C4

Plasmid

#160342

Purpose

High-copy expression vector for K. marxianus, G418 selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pRS314-4M5.3

Plasmid

#45830

Depositor

Dane Wittrup

Insert

4M5.3

Tags

FLAG

Expression

Yeast

Promoter

GAL1

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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pABY-c25

Plasmid

#201769

Purpose

Expresses mNeonGreen-tagged NbALFA under CYC1 promoter in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

NbALFA

Tags

40aa Linker, mNeonGreen

Expression

Yeast

Promoter

CYC1

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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