8,030 results

1. ZJOM40

   Plasmid

   #133650

   Purpose

   Integration of SEC7-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-SEC7-DuDre (SEC7 Budding Yeast)
   

   Use

   Tags

   DuDre

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. GAL FLAG72QDProGFP p306

   Plasmid

   #15833

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 72Q Delta Pro (HTT Human)
   

   Use

   Tags

   FLAG and GFP

   Expression

   Yeast

   Mutation

   Proline-rich region removed

   Promoter

   Available since

   Oct. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pFA6a-link-yoEGFP-SpHis5

   Plasmid

   #44836

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized EGFP with SpHis5 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MmSphk1

   Plasmid

   #118599

   Purpose

   Expresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in mice

   Depositor

   Kevin Lynch

   Article

   Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

   Insert

   Mus musculus sphingosine kinase 1
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   GAL1, 10

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAV0532

   Plasmid

   #133502

   Purpose

   Expression of mTagBFP2 targeted to the nucleus with one NLS signals

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pLys3(BstZ17I)-p(tdh1)-NLSSV40-mTagBFP2-terminator(tdh1)-kanMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHES839

   Plasmid

   #87941

   Purpose

   Constitutively expresses estradiol-inducible synthetic transcriptional regulator in yeast. Transcriptional regulator activates transcription of promoters containing Gal4 binding sites.

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   GAL4 DBD - hER LBD - MSN2 AD (ESR1 Synthetic, Human, Budding Yeast)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 (crippled)

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEK22

   Plasmid

   #210588

   Purpose

   Reporter gene expression in B. dendrobatidis

   Depositor

   Lillian Fritz-Laylin

   Article

   Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   BdH3pro-Hshph-G4S-nanoluc-ScADH1ter
   

   Use

   Luciferase

   Tags

   NanoLuc luciferase

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   BdH3pro

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBJ1806 pRS413-COF1RFP

   Plasmid

   #37101

   Depositor

   John Cooper

   Article

   Lin et al J Cell Sci. 2010 Apr 15;123(Pt 8):1329-4

   Insert

   COF1-RFP
   

   Use

   Tags

   RFP

   Expression

   Yeast

   Mutation

   Single RFP internal insertion at Cof147T48D with …

   Promoter

   Available since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

9. HsSPHK1

   Plasmid

   #118091

   Purpose

   Expresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)

   Depositor

   Kevin Lynch

   Article

   Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

   Insert

   Homo sapiens Sphingosine Kinase 1
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   GAL1, 10

   Available since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNXRVa-HACre

    Plasmid

    #78398

    Purpose

    constitutive Cre expression vector

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    3xHA-nls-Cre
    kanMX6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDRF1-GW yAT1.03

    Plasmid

    #132781

    Purpose

    FRET biosensor for ATP measurements in budding yeast

    Depositor

    Bas Teusink

    Article

    Botman et al ACS Sens. 2020 Mar 27;5(3):814-822. doi:

    Insert

    yAT1.03 ymTq2-tdTomato
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJT130_GalL_nCDA1-NGBE3

    Plasmid

    #145094

    Purpose

    Expressing base editor nCDA1-NGBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    nCDA1-NGBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Yiplac211 proGAL1-TsCC(A)-RGG-GFP-RGG-RGG

    Plasmid

    #176395

    Purpose

    Expresses GFP tagged triple RGG scaffold with N-terminal TsCC(A) coiled coil. Expression induced in media containing galactose.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    GAL1 promoter, TsCC(A)-RGG-GFP-RGG-RGG
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pfa6a PhoCl-TsCC(B)::KANMX

    Plasmid

    #185906

    Purpose

    Plasmid for cloning PhoCl fused to TsCC(B) into yeast.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    PhoCl-TsCC(B)
    

    Use

    Tags

    PhoCl fluorophore with C-terminal TsCC(B)

    Expression

    Yeast

    Mutation

    Promoter

    SP6

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGal4BD-CRY2PHR

    Plasmid

    #28244

    Purpose

    Expresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activation

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    cry2 (CRY2 Mustard Weed)
    

    Use

    Tags

    Gal4BD

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSK221

    Plasmid

    #129249

    Purpose

    reverse yTRAP, tetR-responsive Neon reporter

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    pADH1(tetO2)-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAV0526

    Plasmid

    #133500

    Purpose

    Expression of sfGFP targeted to the nucleus with one NLS signals

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pHis5(StuI)-p(tdh1)-sfGFP-NLSSV40-natMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p416 25Q GPD

    Plasmid

    #1177

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 25Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDRF1-GW yoeGFP-T2A-mCherry

    Plasmid

    #118446

    Purpose

    Produces equimolar expression of yeast codon-optimized eGFP and mCherry

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    yoeGFP-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSII42K

    Plasmid

    #194523

    Purpose

    High copy vector backbone with G418 selectivity, contains MCS

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFA6a-link-yomTagBFP-Kan

    Plasmid

    #44898

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mTagBFP with Kan selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomTagBFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKT-iRFP-KAN

    Plasmid

    #64687

    Purpose

    pKT vector encoding yeast-codon optimized infrared fluorescent protein iRFP.

    Depositor

    Erin O'Shea

    Article

    Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

    Insert

    iRFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Nucleotide sequence has been yeast-codon optimize…

    Promoter

    Available since

    May 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFA6a-GBP-mCherry-kanMX6

    Plasmid

    #89068

    Purpose

    genomic GBP-mCherry epitope C-terminal tagging in S. pombe

    Depositor

    Quanwen Jin

    Article

    Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    GBP-mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBS-SKII-3XHA-HALO-URA

    Plasmid

    #188932

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-HALO-URA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAG424GAL-ccdB

    Plasmid

    #14151

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pNH605-HSE-YFP

    Plasmid

    #172131

    Purpose

    Fluorescent reporter for Hsf1 activity

    Depositor

    Johannes Herrmann

    Article

    Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

    Insert

    4xHSE-YFP
    

    Use

    Vector for genomic integration into the yeast gen…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CYC1 core promotor with 4 tandem repeats of the H…

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPAP_BGL1

    Plasmid

    #183735

    Purpose

    Expresses Aspergillus aculeatus β-glucosidase (BGL1)

    Depositor

    Jun Ishii

    Article

    Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

    Insert

    β-glucosidase (AaBGL1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1 promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCfB2223

    Plasmid

    #67544

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pIM701

    Plasmid

    #74642

    Purpose

    alias pFA6a-ymTagBFP-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and nourseothricin selection marker

    Depositor

    Jiří Hašek

    Article

    Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

    Insert

    ymTagBFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCas9

    Plasmid

    #162606

    Purpose

    Pre-express Cas9 in yeast

    Depositor

    Lars Steinmetz

    Article

    Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

    Insert

    Tef1-Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge