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Showing: 701 - 720 of 7821 results

  4. pWS171

    Plasmid
    #90518
    Purpose
    Cas9 gap repair vector - LEU2
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    90518-plasmid-map-sequence-id-288271

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  5. pJH2972

    Plasmid
    #100956
    Purpose
    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. URA3MX marker
    Depositor
    James Haber
    Article
    Ranjith et al Protocol Exchange (2017)
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    100956-plasmid-map-sequence-id-222978

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  9. pFA6a-hphMX-(3×FLAG)-TEV-ProtA

    Plasmid
    #52692
    Purpose
    For C-terminal tagging of ORFs with a (3×FLAG)-TEV-ProtA
    Depositor
    Michael Nick Boddy
    Insert
    (3×FLAG)-TEV-ProtA
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    n/a
    Available since
    April 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    52692-plasmid-map-sequence-id-82002

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  11. pUDP002

    Plasmid
    #103872
    Purpose
    Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Inserts
    hph
    Spcas9 D147Y P411T
    Use
    CRISPR
    Tags
    NLS
    Expression
    Yeast
    Mutation
    D147Y P411T
    Promoter
    Arxula adeninivorans TEF1 and Ashbya gossypii (Er…
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103872-plasmid-map-sequence-id-199260

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  14. pJRoC30_Th3

    Plasmid
    #188064
    Purpose
    PROSS-designed high-redox potential laccase from Trametes hirsuta
    Depositor
    Sarel Fleishman
    Article
    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173
    Insert
    PROSS-designed high-redox potential laccase from Trametes hirsuta
    Use
    Tags
    S. cerevisiae evolved α-factor prepro-leader sign…
    Expression
    Yeast
    Mutation
    20 mutations, described in publication
    Promoter
    Available since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    188064-plasmid-map-sequence-id-370132

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  15. pJRoC30_Tv9nL

    Plasmid
    #188069
    Purpose
    PROSS-designed high-redox potential laccase from Trametes versicolor
    Depositor
    Sarel Fleishman
    Article
    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173
    Insert
    PROSS-designed high-redox potential laccase from Trametes versicolor
    Use
    Tags
    S. cerevisiae evolved α-factor prepro-leader sign…
    Expression
    Yeast
    Mutation
    79 PROSS mutations, described in publication
    Promoter
    Available since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    188069-plasmid-map-sequence-id-370129

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  17. LT2_14_GlyOx

    Plasmid
    #177711
    Purpose
    Plasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)
    Depositor
    Lisbeth Olsson
    Article
    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d
    Inserts
    pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
    pTEFmut8-mCherry-tADH1
    pTRX2_5xUAS-ymYPET-tCYC1
    Use
    Used as donor dna in genome integration after lin…
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    177711-plasmid-map-sequence-id-378782

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  19. pYZ959

    Plasmid
    #192603
    Purpose
    TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with CEN-URA3
    Depositor
    Jef Boeke
    Article
    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.
    Insert
    Cas9 (codon optimized for human)
    Use
    Synthetic Biology
    Tags
    SV40
    Expression
    Yeast
    Mutation
    Promoter
    ScTEF1
    Available since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    192603-plasmid-map-sequence-id-403767

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  20. pUDP025

    Plasmid
    #103874
    Purpose
    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces lactis
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Insert
    HH-gRNA-HDV targetting ADE2 in Kluyveromyces lactis
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103874-plasmid-map-sequence-id-199263

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Showing: 701 - 720 of 7821 results