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8,418 results

Showing: 701 - 720 of 8418 results

  1. ZJOM140

    Plasmid
    #133668
    Purpose
    Integration of COX4-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.
    Depositor
    Zhiping Xie
    Publication
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-COX4-mTagBFP2 (COX4 Budding Yeast)
    Tags
    mTagBFP2
    Expression
    Yeast
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    133668-plasmid-map-sequence-id-271502

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  3. ZJOM75

    Plasmid
    #133649
    Purpose
    Integration of ANP1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.
    Depositor
    Zhiping Xie
    Publication
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-ANP1-mCherry (ANP1 Budding Yeast)
    Tags
    mCherry
    Expression
    Yeast
    Available since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133649-plasmid-map-sequence-id-267445

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  4. BB3cK_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104908
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    104908-plasmid-map-sequence-id-206067

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  8. pHIPH4

    Plasmid
    #117685
    Purpose
    Hansenula polymorpha expression plasmid
    Depositor
    Ida van der Klei
    Insert
    Alcohol Oxidase promoter
    Expression
    Yeast
    Promoter
    Alcohol Oxidase
    Available since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    117685-plasmid-map-sequence-id-232223

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  9. pV1025

    Plasmid
    #111424
    Purpose
    Expression of Candida albicans codon-optimized Cas9 (CaCas9) - integrates at ENO1 (Part of Duet system)
    Depositor
    Gerald Fink
    Publication
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    CaCas9
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111424-plasmid-map-sequence-id-212243

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  11. pJO629

    Plasmid
    #104342
    Purpose
    Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.
    Depositor
    Alexander Varshavsky
    Publication
    Oh et al J Biol Chem. 2017 Dec 29;292(52):21457-2
    Insert
    pTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
    Tags
    3xFLAG
    Expression
    Yeast
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    104342-plasmid-map-sequence-id-207903

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  12. pPICZc-HsACTG1*-thymosinB-8His

    Plasmid
    #111147
    Purpose
    Expresses human gamma-actin (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag.
    Depositor
    Mohan Balasubramanian
    Publication
    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827
    Insert
    actin gamma 1 (ACTG1 Human)
    Use
    Pichia pastoris integration vector
    Tags
    Thymosin beta and a His-tag
    Expression
    Yeast
    Mutation
    codon-optimised for expression in Pichia pastoris
    Promoter
    AOX1
    Available since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    111147-plasmid-map-sequence-id-214068

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  15. pDB4281

    Plasmid
    #98700
    Purpose
    A Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX system
    Depositor
    Li-Lin Du
    Publication
    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    98700-plasmid-map-sequence-id-182829

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  16. pDB4283

    Plasmid
    #98702
    Purpose
    A plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX system
    Depositor
    Li-Lin Du
    Publication
    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.
    Insert
    gRNA PCR template
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    98702-plasmid-map-sequence-id-182828

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  17. pPMVAu8

    Plasmid
    #98297
    Purpose
    Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of consitutive promoters.
    Depositor
    Claudia Vickers
    Publication
    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.
    Insert
    PRPL4A-EfmvaS-TEFM1-PRPL15A-EfmvaE -TEBS1
    Expression
    Yeast
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98297-plasmid-map-sequence-id-189970

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Showing: 701 - 720 of 8418 results