8,400 results
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Plasmid#126717Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
ExpressionYeastPromoterGAPAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCggRO-reporter
Plasmid#124582PurposeReporter plasmid with Constitutively expressed mCherry and CggR-regulated YFP (eCitrine)DepositorInsertCggR promoter element
ExpressionYeastAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJW1664
Plasmid#112038PurposeCEN/ARS plasmid expression of mitochondrial Su9-TagBFP marked with HISDepositorInsertSu9
TagsTagBFPExpressionYeastMutationamino acids 1-76Available SinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHIPH4
Plasmid#117685PurposeHansenula polymorpha expression plasmidDepositorInsertAlcohol Oxidase promoter
ExpressionYeastPromoterAlcohol OxidaseAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pV1025
Plasmid#111424PurposeExpression of Candida albicans codon-optimized Cas9 (CaCas9) - integrates at ENO1 (Part of Duet system)DepositorInsertCaCas9
UseCRISPRExpressionYeastAvailable SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTXRE5-Z
Plasmid#112509PurposeReporter plasmid, responsive to activated Ah receptor/ArntDepositorInsertRW95-3 with 5x xenobiotic response elements (lacZ E.coli, Synthetic)
ExpressionYeastPromoter5 XREs+ minimal Cytochrome CAvailable SinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJO629
Plasmid#104342PurposeExpress yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertpTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
Tags3xFLAGExpressionYeastAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUG-natNT2
Plasmid#110922Purposeexpression of nat gene conferring resistance to nourseothricin for gene deletion in yeastDepositorInsertnat
ExpressionBacterial and YeastPromoterAgTEF1Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6-mTFP1-KanMX6
Plasmid#69851PurposeC-terminal tagging with mTFP1 using KanMX6 as a selection marker in S. cerevisiae.DepositorInsertmTFP1
ExpressionYeastAvailable SinceApril 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-yoHaloTag-TLG1
Plasmid#105277PurposePlasmid for pop-in/pop-out replacement of Tlg1 with yoHaloTag-Tlg1.DepositorInsertTlg1 (TLG1 Budding Yeast)
TagsyoHaloTagExpressionYeastMutationHaloTag has yeast optimized codonsAvailable SinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4281
Plasmid#98700PurposeA Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX systemDepositorInsertCas9
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4283
Plasmid#98702PurposeA plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX systemDepositorInsertgRNA PCR template
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPMVAu8
Plasmid#98297PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPRPL4A-EfmvaS-TEFM1-PRPL15A-EfmvaE -TEBS1
ExpressionYeastAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mEOS3.2-KanMX6
Plasmid#87030PurposeTag S. pombe genes in C terminal with mEOS3.2DepositorInsertmEOS3.2
ExpressionBacterial and YeastAvailable SinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCfB3052(gRNA X-4, XI-3, XII-5)
Plasmid#73294PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFM699
Plasmid#74636Purposealias pFA6a-mCherry-natNT2 - vector for C-terminal tagging of yeast genes with mCherry and nourseothricin selection markerDepositorInsertmCherry
ExpressionYeastPromoternoneAvailable SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRR-AR
Plasmid#73045PurposeConstitutively expresses the androgen receptor, which is required for the activation of the promoter in pLAREG (Addgene plasmid #73045)DepositorInsertAndrogen receptor (AR Human)
ExpressionYeastMutationplease see depositor comments belowPromoterglyceraldehyde phosphate dehydrogenase (GPD)Available SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
BS-Ura3-GFP-Atg8
Plasmid#69194PurposeSingle copy integration of GFP-Atg8, use auxotrophic marker URA3(Kluyveromyces lactis)DepositorInsertGFP-Atg8
TagsEGFPExpressionYeastAvailable SinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRG206
Plasmid#64527PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIYC04
Plasmid#64741PurposeYeast expression plasmidDepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only