Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

Your Selections

Yeast Expression

7,935 results

Showing: 2401 - 2450 of 7935 results

  5. pFA6a-PATagRFP-KanMX6

    Plasmid
    #87027
    Purpose
    Tag S. pombe genes in C terminal with PATagRFP
    Depositor
    Julien Berro
    Insert
    PATagRFP
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Feb. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    87027-plasmid-map-sequence-id-172998

    Enlarge

  10. pDIV494

    Plasmid
    #177701
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    sgRNA Targeting ADE2 locus in D.hansenii: AGCTAAGCAGATTAATGCAT
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    RNR2p (Debaryomyces hansenii ), SNR52p (Candida s…
    Available since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177701-plasmid-map-sequence-id-352589

    Enlarge

  11. pMC6-TEFkFLP(LYS2)

    Plasmid
    #49458
    Purpose
    overespression of Flp step-arrest mutant in yeast, LYS2 selection
    Depositor
    Marc Gartenberg
    Insert
    Flp(H305L) (flp Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    H305L, ‘step-arrest’ mutant,
    Promoter
    TEF1
    Available since
    Dec. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    49458-plasmid-map-sequence-id-73670

    Enlarge

  14. pAAH6

    Plasmid
    #41830
    Purpose
    Used as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for ClonNAT resistance
    Depositor
    Melissa Moore
    Article
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    T7,TEF
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    41830-plasmid-map-sequence-id-69212

    Enlarge

  16. pGBW-m4046299

    Plasmid
    #153878
    Purpose
    Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153878-plasmid-map-sequence-id-300422

    Enlarge

  17. pAG416GALL-HAV_3C

    Plasmid
    #203481
    Purpose
    Expresses HAV 3C protease from a GALL promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    HAV 3C protease
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GALL
    Available since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    203481-plasmid-map-sequence-id-402754

    Enlarge

  22. HsSPHK2

    Plasmid
    #118092
    Purpose
    Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)
    Depositor
    Kevin Lynch
    Article
    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do
    Insert
    Homo sapiens Sphingosine Kinase 2 (SPHK2 Human)
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, 10
    Available since
    Aug. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    118092-plasmid-map-sequence-id-234125

    Enlarge

  23. pFA0055

    Plasmid
    #131774
    Purpose
    Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.
    Depositor
    Frank Albert
    Article
    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.
    Insert
    20mer CASS5a guide (gCASS5a) and 5' sgRNA
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    131774-plasmid-map-sequence-id-258731

    Enlarge

  26. pBS10

    Plasmid
    #83795
    Purpose
    Useful for tagging yeast proteins with C-terminal Cerulean tag; hygromycin-B selection
    Depositor
    Eric Muller
    Article
    Hailey et al Methods Enzymol. 2002;351:34-49.
    Insert
    Cerulean
    Use
    Tags
    Cerulean
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    83795-plasmid-map-sequence-id-160032

    Enlarge

  34. pFTK093

    Plasmid
    #171365
    Purpose
    Vector part (LVL1) from the Fungal Modular Cloning ToolKit.
    Depositor
    Arnold Driessen
    Article
    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021
    Insert
    sgRNA transcription unit (MoClo lvl1 unit), P-gpdA-HH-sgRNA-HDV-Ttrpc, replacable LacZ gene
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    n/a
    Promoter
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    171365-plasmid-map-sequence-id-352204

    Enlarge

  39. pCfB6684

    Plasmid
    #106154
    Purpose
    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6631, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106154-plasmid-map-sequence-id-206237

    Enlarge

  40. pCfB6631

    Plasmid
    #106161
    Purpose
    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6684 into Yarrowia lipolytica chromosomal location IntD_1, amp resistance
    Depositor
    Irina Borodina
    Article
    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/
    Insert
    unknown
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106161-plasmid-map-sequence-id-206246

    Enlarge

Showing: 2401 - 2450 of 7935 results