8,021 results

1. pCR

   Plasmid

   #60619

   Purpose

   crRNA expression plasmid on pRS423 backbone

   Depositor

   Huimin Zhao

   Article

   Bao et al ACS Synth Biol. 2014 Sep 19.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUCdeltaSS-ERE

   Plasmid

   #108217

   Purpose

   Yeast reporter gene with estrogen response element (ERE) upstream of CYC1 promoter driving lacZ expression

   Depositor

   Didier Picard, Keith Yamamoto

   Article

   Picard et al Nature 1990 Nov 8;348(6297):166-8.

   Insert

   ERE-CYC1-lacZ
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CYC1

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBS4

   Plasmid

   #83793

   Purpose

   Useful for tagging yeast proteins with C-terminal CFP tag; hygromycin-B selection

   Depositor

   Eric Muller

   Article

   Hailey et al Methods Enzymol. 2002;351:34-49.

   Insert

   CFP
   

   Use

   Tags

   CFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 303Gal-FUS

   Plasmid

   #29614

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 18, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pH-eCFP

   Plasmid

   #176555

   Purpose

   Expression of eCFP for auxotrophic selection in the absence of histidine

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   eCFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pL-eCFP

   Plasmid

   #176556

   Purpose

   Expression of eCFP for auxotrophic selection in the absence of leucine

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   eCFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pU-eCFP

   Plasmid

   #176557

   Purpose

   Expression of eCFP for auxotrophic selection in the absence of uracil

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   eCFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFTK043

   Plasmid

   #171315

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   eGFP fluorescent reporter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Promoter

   Available since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS406-PHO5-GFP-2xOsh2 PH domain [short]

   Plasmid

   #58829

   Purpose

   Expresses GFP-Tagged Osh2 PH domain in yeast

   Depositor

   Tim Levine

   Article

   Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

   Insert

   2xOsh2 PH domain [short] (OSH2 Budding Yeast)
   

   Use

   Tags

   GFP and Myc

   Expression

   Yeast

   Mutation

   Promoter

   PHO5

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pKL08

    Plasmid

    #104432

    Purpose

    pHuji in yeast expression vector

    Depositor

    Joel Kralj

    Article

    Dodd et al Sci Rep. 2017 Oct 24;7(1):13922. doi: 10

    Insert

    pHuji
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tef1

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMJA149

    Plasmid

    #128524

    Purpose

    Expresses mutant Ber e 1 in Pichia

    Depositor

    Marcos Alcocer

    Insert

    mutant Ber e 1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAG413GAL-ccdB-TAP

    Plasmid

    #14261

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    TAP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 413-Met25 Kar2 TorsinA GFP

    Plasmid

    #32119

    Depositor

    Susan Lindquist

    Article

    Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

    Insert

    TorsinA (TOR1A Human)
    

    Use

    Tags

    GFP and Kar2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pHES841

    Plasmid

    #89194

    Purpose

    Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    pZ-YFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pZ

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGBW-m4046633

    Plasmid

    #149161

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF3a protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. YIplac204-pOst1-ESCargo

    Plasmid

    #115420

    Purpose

    Expresses a regulatable secretory cargo for yeast at the TRP1 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

    Insert

    pOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. prs316 CUP-GFP

    Plasmid

    #128052

    Purpose

    Yeast expression vector for N terminal GFP fusions

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDS272

    Plasmid

    #34975

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2-GFP-LOVpep (MID2 A. sativa, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T406A, T407A

    Promoter

    TEF

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pK3F-GAL1

    Plasmid

    #85772

    Purpose

    N-ICE plasmid pK3F-GAL1

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-KanMX-loxP-GAL1p-3FLAG
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFA6a-link-yomApple-SpHis5

    Plasmid

    #44844

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mApple with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomApple
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pKT0139

    Plasmid

    #8731

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    yECitrine
    

    Use

    Tags

    Codon optimized linker

    Expression

    Yeast

    Mutation

    yEGFP with S65G, V68L, Q69M, S72A, T203Y

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pL-rpb1-N488D

    Plasmid

    #10988

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    rpb1-N488D (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Mutation 1462 A to G in the RPB1 ORF resulting in…

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

23. pSc-His6-Sem1Sc

    Plasmid

    #83235

    Purpose

    pGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeast

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Insert

    Sem 1 (SEM1 Budding Yeast)
    

    Use

    Tags

    His6

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCfB2899(X-2 MarkerFree)

    Plasmid

    #73271

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAG423GAL-EGFP-ccdB

    Plasmid

    #14317

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. M4758 KanMX::URA3 Disruptor Converter

    Plasmid

    #51688

    Purpose

    Yeast marker swap plasmid for converting KanMX3 to URA3

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    URA3 (URA3 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJAS1052

    Plasmid

    #158598

    Purpose

    VioA, VioB

    Depositor

    John Dueber

    Article

    Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

    Inserts

    VioA
    VioB
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRG213MX

    Plasmid

    #64522

    Purpose

    Centromeric E. coli/S. cerevisiae shuttle vector

    Depositor

    Joerg Stelling

    Article

    Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PhyB-mCherry-Spc72

    Plasmid

    #51582

    Purpose

    Constitutively express spindle pole body localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Use

    Tags

    Spc72 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. YIplac211-APL2-iGFPx6

    Plasmid

    #105251

    Purpose

    Plasmid for pop-in/pop-out replacement of Apl2 with Apl2-iGFPx6.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    APL2 portion (APL2 Budding Yeast)
    

    Use

    Tags

    iGFPx6

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pU-mRuby2

    Plasmid

    #176549

    Purpose

    Expression of mRuby2 for auxotrophic selection in the absence of uracil

    Depositor

    Markus Ralser

    Article

    Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

    Insert

    yomRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH1(GAP)

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pH-mRuby2

    Plasmid

    #176547

    Purpose

    Expression of mRuby2 for auxotrophic selection in the absence of histidine

    Depositor

    Markus Ralser

    Article

    Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

    Insert

    yomRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH1(GAP)

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pXP322

    Plasmid

    #26839

    Depositor

    Nancy DaSilva, Suzanne Sandmeyer

    Article

    Fang et al Yeast. 2010 Sep 10. ():.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFA6a-12Pk-ura4MX6

    Plasmid

    #49180

    Purpose

    genomic 12Pk epitope tagging in S. pombe

    Depositor

    Eishi Noguchi

    Article

    Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

    Type

    Empty backbone

    Use

    Tags

    12Pk

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFA6a-link-yomRuby-SpHis5

    Plasmid

    #44843

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mRuby with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomRuby
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. LD113

    Plasmid

    #69166

    Purpose

    Integration of GFP tag at Atg13 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg13-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pV1464

    Plasmid

    #111440

    Purpose

    N. castellii Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHES874

    Plasmid

    #89196

    Purpose

    Expresses mCherry under control of synthetic promoter containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    pZ-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pZ

    Available since

    Oct. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pV2A-T

    Plasmid

    #153022

    Purpose

    Single-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoter

    Depositor

    Vito Valiante

    Article

    Hoefgen et al Metab Eng. 2018 Jul;48:44-51. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCfB2226

    Plasmid

    #67533

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae X-4 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge