7,935 results

1. PhyB-mCherry-Spc72

   Plasmid

   #51582

   Purpose

   Constitutively express spindle pole body localized PhyB in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Use

   Tags

   Spc72 and mCherry

   Expression

   Yeast

   Mutation

   1-908aa only

   Promoter

   ADH1pr

   Available since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB2803

   Plasmid

   #63646

   Purpose

   pCfB2803 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

   Depositor

   Irina Borodina, Jerome Maury

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pV2A-T

   Plasmid

   #153022

   Purpose

   Single-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoter

   Depositor

   Vito Valiante

   Article

   Hoefgen et al Metab Eng. 2018 Jul;48:44-51. doi: 10.10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Oct. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKT0139

   Plasmid

   #8731

   Depositor

   Kurt Thorn

   Article

   Sheff et al Yeast 2004 Jun;21(8):661-70.

   Insert

   yECitrine
   

   Use

   Tags

   Codon optimized linker

   Expression

   Yeast

   Mutation

   yEGFP with S65G, V68L, Q69M, S72A, T203Y

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-12Pk-ura4MX6

   Plasmid

   #49180

   Purpose

   genomic 12Pk epitope tagging in S. pombe

   Depositor

   Eishi Noguchi

   Article

   Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

   Type

   Empty backbone

   Use

   Tags

   12Pk

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDS272

   Plasmid

   #34975

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   Mid2-GFP-LOVpep (MID2 Budding Yeast, A. sativa)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   T406A, T407A

   Promoter

   TEF

   Available since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHES874

   Plasmid

   #89196

   Purpose

   Expresses mCherry under control of synthetic promoter containing Zif268 binding sites.

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   pZ-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pZ

   Available since

   Oct. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pXP322

   Plasmid

   #26839

   Depositor

   Suzanne Sandmeyer, Nancy DaSilva

   Article

   Fang et al Yeast. 2010 Sep 10. ():.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGAN101

   Plasmid

   #131123

   Purpose

   Plasmid for overexpression of prion domains to induce prions

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Type

   Empty backbone

   Use

   Tags

   mKate2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. M4758 KanMX::URA3 Disruptor Converter

    Plasmid

    #51688

    Purpose

    Yeast marker swap plasmid for converting KanMX3 to URA3

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    URA3 (URA3 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHES609 [prNOp7-PBAC]

    Plasmid

    #103017

    Purpose

    A S. cerevisiae plasmid. A photosensitive adenylate cyclase that produces cAMP in response to blue light. Used to activate PKA.

    Depositor

    Hana El-Samad

    Article

    Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

    Insert

    bPAC
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMJB1

    Plasmid

    #160429

    Purpose

    Yeast vector for expressing mCherry tagged genes under the GAL1 promoter

    Depositor

    Gary Stormo

    Article

    Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

    Type

    Empty backbone

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGBW-m4046494

    Plasmid

    #149137

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 surface glycoprotein (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. YIplac211-APL5-mCherry2Bx6

    Plasmid

    #105253

    Purpose

    Plasmid for pop-in/pop-out replacement of Apl5 with Apl5-mCherry2Bx6.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Apl5 (APL5 Budding Yeast)
    

    Use

    Tags

    mCherry2Bx6

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pICE LEU2

    Plasmid

    #122878

    Purpose

    LEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pXP216

    Plasmid

    #26830

    Depositor

    Suzanne Sandmeyer, Nancy DaSilva

    Article

    Fang et al Yeast. 2010 Sep 10. ():.

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCETT

    Plasmid

    #19782

    Depositor

    Jumi Shin

    Article

    Chen et al Biotechniques. 2008 Sep . 45(3):295-304.

    Type

    Empty backbone

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDIV489

    Plasmid

    #177697

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RNR2p (Debaryomyces hansenii ) and TDH3p (Candida…

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA6a-link-yoPA-mCherry-SpHis5

    Plasmid

    #44855

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized photoactivatable mCherry with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoPA-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pESC-LEU-SopB

    Plasmid

    #183670

    Purpose

    Inducible Salmonella Typhimurium SopB for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pESC-Leu-SopB(C460S)

    Plasmid

    #183671

    Purpose

    Inducible Salmonella Typhimurium SopB (catalytically inactive) for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C460S

    Promoter

    GAL1

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDRF1-GW mScarletI-T2A-mTurquoise2

    Plasmid

    #118430

    Purpose

    Produces equimolar expression of mScarletI and mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    mScarletI-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFA6a-link-yomApple-CaURA3

    Plasmid

    #44879

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mApple with CaUra3 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomApple
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRS416-Aga1p-FAPB2.3.6

    Plasmid

    #158147

    Purpose

    Yeast display reporter for non-display yeast strains (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    Aga1p-FAPB2.3.6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. YIplac211-RPL13A-FKBPx2

    Plasmid

    #104473

    Purpose

    Plasmid for pop-in/pop-out replacement of RPL13A with an FKBPx2-tagged version.

    Depositor

    Benjamin Glick

    Article

    Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

    Insert

    RPL13Ap (RPL13A Budding Yeast)
    

    Use

    Tags

    FKBPx2-HA

    Expression

    Yeast

    Mutation

    includes the last 354 bp of RPL13A only

    Promoter

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJK59-ATG18-GFP

    Plasmid

    #42529

    Depositor

    James Hurley

    Article

    Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi:

    Insert

    Atg18 (ATG18 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    none

    Promoter

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pDH3

    Plasmid

    #83789

    Purpose

    Useful for tagging yeast proteins with C-terminal CFP tag; G418 selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    CFP
    

    Use

    Tags

    CFP

    Expression

    Yeast

    Mutation

    F64L, Y66W, N146I, M153T, V163A, N164H mutations …

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAG413GPD-EGFP-ccdB

    Plasmid

    #14310

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRS415-KEX2

    Plasmid

    #10996

    Depositor

    Francisco Malagon

    Insert

    KEX2 (KEX2 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Wild type

    Promoter

    Available since

    Dec. 29, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMEL15

    Plasmid

    #107921

    Purpose

    nat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. THI VP64 pGAP T-

    Plasmid

    #161487

    Purpose

    Non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Non targeting control NTC)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pB194

    Plasmid

    #61154

    Purpose

    Expression of KIN28 under GAL1 promoter in yeast

    Depositor

    Charles Brenner

    Article

    Bieganowski et al J Biol Chem. 2002 Mar 29;277(13):10852-6

    Insert

    TFIIH complex serine/threonine-protein kinase subunit KIN28 (KIN28 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal1

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

33. pRPR1(1xTetO)_gRNA_handle_RPR1t

    Plasmid

    #62966

    Purpose

    aTc inducible gRNA expression vector

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    RPR1

    Available since

    March 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pR5-28

    Plasmid

    #22289

    Depositor

    Louise Prakash

    Article

    Pages et al Genetics. 2008 Sep . 180(1):73-82.

    Insert

    RAD5 (RAD5 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

35. pROS14

    Plasmid

    #107928

    Purpose

    LEU2 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. RPL3-GFP (96)

    Plasmid

    #24040

    Depositor

    Michael Rout

    Insert

    RPL3 (RPL3 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

37. pCfB2190

    Plasmid

    #67535

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae XI-2 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDR-GW FLIPglu-170nΔ13V

    Plasmid

    #27999

    Depositor

    Wolf Frommer

    Article

    Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

    Insert

    FLIPglu-170nΔ13V
    

    Use

    Tags

    eCFP and eYFP

    Expression

    Yeast

    Mutation

    WT binding protein (no F16A)

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

39. pMC-Ura-Cen-lvl1

    Plasmid

    #176182

    Purpose

    preassembled yeast MoClo level-1 backbone with GFP dropout and URA3 marker

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMC-X2

    Plasmid

    #176152

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-2

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge