7,935 results
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Plasmid#133899PurposeNegative control. Expression of Gal4BD-Lam hybrid protein. Homology regions for recombination with pAWHDepositorInsertLMNA (Lam) (LMNA Human)
UseTagsExpressionYeastMutationPromoterT7Available sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-ccdB-DsRed
Plasmid#14363DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHB4
Plasmid#53462PurposeThis plasmid contains an ADHpr driven chimeric protein with SNC1, N-terminal GFP and C-terminal SUC2. It contains flanking homology to SUC2, allowing optional integration.DepositorInsertyeGFP-Snc1-Suc2
UseTagsExpressionYeastMutationPromoterADHAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pDZ536 pURA ADE3p PP7-PS-2x-yeGFP
Plasmid#72234PurposeUracil selection; Expression of PP7-2x-yeGFP fusion protein under the ADE3 promoterDepositorInsertPP7-PS-2x-yeGFP
UseTagsExpressionYeastMutationPromoterADE3pAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDN-T2dGZmxh
Plasmid#44552DepositorInsertsPTETREG promoter
yEGFP::ZeoR
UseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationPromoterPTETREGAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4I
Plasmid#104504PurposeUsed to evaluate the expression output of Saccharomyces kudriavzevii GAL1 promoter with yEGFP used as the reporter geneDepositorInsertSkGAL1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4Q
Plasmid#104512PurposeUsed to evaluate the expression output of Saccharomyces kudriavzevii GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSkGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG414GAL-EGFP-ccdB
Plasmid#14311DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHLK
Plasmid#64182Purposeharbours marker genes HIS3, LEU2 and LYS2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCfB3047(gRNA XII-1)
Plasmid#73289PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-1DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK369
Plasmid#73594PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitopeDepositorInsertdelta-12 fatty acid acetylenase
UseTagsFLAG tagExpressionYeastMutationPromoterGAL10Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-CERT PH domain
Plasmid#58725PurposeExpresses GFP-Tagged CERT PH domain in yeastDepositorInsertcollagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
UseTagsGFP and MycExpressionYeastMutationPromoterPHO5Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBBK92 pHomL-TDH3p-Scarlet(rfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)
Plasmid#179076PurposeVector containing a yeast integration cassette for expressing TDH3p-Scarlet(rfp)-Link-SCS2tmh, an overexpressed (bright) marker for the endoplasmic reticulumDepositorInsertTDH3p-Scarlet(rfp)-Link-SCS2tmh
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK89 pHomL-TDH3p-Scarlet(rfp)-Link-2xPH-SSA1t-HomR (AmpR)
Plasmid#179073PurposeVector containing a yeast integration cassette for expressing TDH3p-Scarlet(rfp)-Link-2xPH, an overexpressed (bright) marker for the plasma membraneDepositorInsertTDH3p-Scarlet(rfp)-Link-2xPH
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK87 pHomL-RPL18Bp-TOM70-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179071PurposeVector containing a yeast integration cassette for RPL18B-driven expression of TOM70-Link-Scarlet(rfp), a marker for the mitochondriaDepositorInsertRPL18Bp-TOM70-Link-Scarlet(rfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK85 pHomL-RPL18Bp-PEX25-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179069PurposeVector containing a yeast integration cassette for RPL18B-driven expression of PEX25-Link-Scarlet(rfp), a marker for the peroxisomeDepositorInsertRPL18Bp-PEX25-Link-Scarlet(rfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK81 pHomL-RPL18Bp-PRC1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179065PurposeVector containing a yeast integration cassette for RPL18B-driven expression of PRC1-Link-Scarlet(rfp), a marker for the vacuoleDepositorInsertRPL18Bp-PRC1-Link-Scarlet(rfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK78 pHomL-RPL18Bp-SNF7-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179062PurposeVector containing a yeast integration cassette for RPL18B-driven expression of SNF7-Link-Scarlet(rfp), a marker for the endosome, class E compartmentDepositorInsertRPL18Bp-SNF7-Link-Scarlet(rfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK77 pHomL-RPL18Bp-Scarlet(rfp)-Link-VPS21-SSA1t-HomR (AmpR)
Plasmid#179061PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-VPS21, a marker for the endosomesDepositorInsertRPL18Bp-Scarlet(rfp)-Link-VPS21
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK75 pHomL-RPL18Bp-Scarlet(rfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR)
Plasmid#179059PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-2xPH(PLCd), a marker for the plasma membraneDepositorInsertRPL18Bp-Scarlet(rfp)-Link-2xPH(PLCd)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK74 pHomL-RPL18Bp-Scarlet(rfp)-Link-SFT2-SSA1t-HomR (AmpR)
Plasmid#179058PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SFT2, a marker for the late GolgiDepositorInsertRPL18Bp-Scarlet(rfp)-Link-SFT2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK73 pHomL-RPL18Bp-Scarlet(rfp)-Link-SED5-SSA1t-HomR (AmpR)
Plasmid#179057PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SED5, a marker for the early GolgiDepositorInsertRPL18Bp-Scarlet(rfp)-Link-SED5
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK72 pHomL-RPL18Bp-Scarlet(rfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)
Plasmid#179056PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SCS2tmh, a marker for the endoplasmic reticulumDepositorInsertRPL18Bp-Scarlet(rfp)-Link-SCS2tmh
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK71 pHomL-RPL18Bp-ARC35-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179055PurposeVector containing a yeast integration cassette for RPL18B-driven expression of ARC35-Link-Scarlet(rfp), a marker for the actin cytoskeletonDepositorInsertRPL18Bp-ARC35-Link-Scarlet(rfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK69 pHomL-RPL18Bp-SPC42-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179053PurposeVector containing a yeast integration cassette for RPL18B-driven expression of SPC42-Link-Scarlet(rfp), a marker for the spindle poleDepositorInsertRPL18Bp-SPC42-Link-Scarlet(rfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK12 pCAS-Tyr-[gRNA: 2=FF21] (SplitKanR, AmpR)
Plasmid#178996PurposeSp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitKanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046426
Plasmid#149140PurposeYeast Expression plasmid for SARS-CoV-2 ORF7bDepositorInsertSARS-CoV-2 ORF7b (ORF7b Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046422
Plasmid#145515PurposeYeast Expression plasmid for SARS-CoV-2 ORF6 proteinDepositorInsertSARS-CoV-2 ORF6 protein (ORF6 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCfB2802
Plasmid#63655PurposepCfB2802 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
CUP Rnq1 YFP p316
Plasmid#15596DepositorInsertRNQ1 (RNQ1 Budding Yeast)
UseTagsYFPExpressionYeastMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis
Plasmid#60390PurposeExpression of yeast alpha tubulin (TUB1) - internal 6xHis using a GAL promoterDepositorInsertTUB1 (TUB1 Budding Yeast)
UseTagsExpressionYeastMutationinternal 6xHis (see supplement figure 4)PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-VPS16-iGFPx6
Plasmid#105275PurposePlasmid for pop-in/pop-out replacement of Vps16 with Vps16-iGFPx6.DepositorInsertVps16 portion (VPS16 Budding Yeast)
UseTagsiGFPx6ExpressionYeastMutationPromoterAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
416Gal-FUS-H517Q-YFP
Plasmid#29620DepositorInsertFUS (FUS Human)
UseTagsYFPExpressionYeastMutationH517QPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-APL5-iGFPx6
Plasmid#105252PurposePlasmid for pop-in/pop-out replacement of Apl5 with Apl5-iGFPx6.DepositorInsertApl5 (APL5 Budding Yeast)
UseTagsiGFPx6ExpressionYeastMutationPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBTM116-D9
Plasmid#111232Purposeyeast expression vectorDepositorInsertccdB-cassette
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXP314
Plasmid#26835DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fCLIP-Kan
Plasmid#188923PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fCLIP-Kan
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB305-K3L-vac-HA
Plasmid#20054DepositorInsertK3L
UseTagsHAExpressionYeastMutationPromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pXRLy2
Plasmid#63149PurposeLys2 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG423GAL-ccdB-EYFP
Plasmid#14221DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
p413GAL1-Zipp.-L-IFP-F[2] (Yeast)
Plasmid#52893PurposePlasmid+insert encoding GCN4-leucine zipper fused to IFP-F[2] by the linker (GGGGS) X 2. Zipper-L-IFP-F[2] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[2]
UseTagsL-IFP-F[2]ExpressionYeastMutationPromoterGAL1Available sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-3nmt1-mMaple3
Plasmid#105167PurposeYeast gene targetingDepositorInsertkanMX6-3nmt1-mMaple3
UseTags3nmt1-mMaple3ExpressionBacterial and YeastMutationPromoter3nmt1Available sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG423GPD-ccdB-DsRed
Plasmid#14366DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastMutationPromoterAvailable sinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
YN2_1_LT84_RPL13A
Plasmid#177712PurposeExpresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiaeDepositorInsertCas9
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNIA-CEN-FLAG-LINXa4(RLR)-Bre1
Plasmid#116884PurposeFLAG-tagged LINXa4-Bre1 with RLR hinge-loop mutationsDepositorInsertLINXa4(RLR)-Bre1
UseSynthetic BiologyTagsFLAGExpressionYeastMutationBre1 point mutations K8A, K9A and K11A, LINXa3 wi…PromoterADH1Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-VSV-G-hphMX4
Plasmid#20773DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionYeastMutationPromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pRG221
Plasmid#64520PurposeEpisomal E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only