7,935 results

1. pAG413GPD-ccdB-HA

   Plasmid

   #14238

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   3HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRS-Dhh1-PA(D195A,E196A,S226A,T228A)(405)

   Plasmid

   #122067

   Purpose

   Expressing Dhh1-PA

   Depositor

   Daniel Klionsky

   Article

   Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

   Insert

   Dhh1-PA (DHH1 Budding Yeast)
   

   Use

   Tags

   PA

   Expression

   Yeast

   Mutation

   Aspartic acid 195 to alanine; Glutamic acid 196 t…

   Promoter

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FRP1483

   Plasmid

   #64511

   Purpose

   Expresses destabilized Ametrine in yeast.

   Depositor

   Joerg Stelling

   Article

   Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

   Insert

   Ametrine-ADH1-tail
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZCAN

   Plasmid

   #129115

   Purpose

   Z. rouxii centromeric vector containing ClonNAT resistance

   Depositor

   Paolo Giudici

   Article

   Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFA6a-FHyTkAX

   Plasmid

   #73901

   Purpose

   fission yeast drug counter-selectable selection marker

   Depositor

   Kazunori Tomita

   Article

   Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

   Insert

   hyg-TK
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMJA181

   Plasmid

   #128539

   Purpose

   Expresses anti-Prunus dulcis scFv Ab+BirA tag

   Depositor

   Marcos Alcocer

   Article

   de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

   Insert

   scFv Ab clone PD1F6 plus BirA tag
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBBK86 pHomL-RPL18Bp-preCOX4-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179070

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of preCOX4-Link-Scarlet(rfp), a marker for the mitochondria

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-preCOX4-Link-Scarlet(rfp)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBBK68 pHomL-RPL18Bp-SRM1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179052

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of SRM1-Link-Scarlet(rfp), a marker for the nucleus

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-SRM1-Link-Scarlet(rfp)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. LD101

   Plasmid

   #69160

   Purpose

   Integration of GFP tag at Atg1 C terminus, use auxotrophic marker URA3(Candida albicans)

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   Atg1-EGFP
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)

    Plasmid

    #86421

    Purpose

    N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequence

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    CDC42pr-KAR2SS-GFP1-10
    

    Use

    Tags

    GFP1-10

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRS414 Hsh155 K410N

    Plasmid

    #87280

    Purpose

    Encodes Hsh155 K410N for expression in yeast

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

    Insert

    HSH155 (HSH155 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    K410N

    Promoter

    Available since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRS426-ACT_stable

    Plasmid

    #127609

    Purpose

    high-copy URA-selectable plasmid, Mut ACT1 intron (stable) inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    Stable ACT1 intron (Mut)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. YIPlac204-TC-Sec7-6xDsRed-M1

    Plasmid

    #25448

    Depositor

    Benjamin Glick

    Article

    Losev et al Nature. 2006 Jun 22. 441(7096):1002-6.

    Insert

    Sec7 (SEC7 Budding Yeast)
    

    Use

    Tags

    6xDsRed.M1

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAG306GPD-ccdB-HA

    Plasmid

    #14236

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS405 PEX14-VAC8-RFP

    Plasmid

    #166844

    Purpose

    Expresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORF

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

    Insert

    VAC8 (VAC8, PEX14 Budding Yeast)
    

    Use

    Tags

    PEX14 transmembrane domain and RFP

    Expression

    Yeast

    Mutation

    PEX14 transmembrane domain

    Promoter

    VAC8 promoter

    Available since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS405 SEC66-VAC8-RFP

    Plasmid

    #166843

    Purpose

    Expresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

    Insert

    VAC8 (VAC8, SEC66 Budding Yeast)
    

    Use

    Tags

    RFP and SEC66 transmembrane domain

    Expression

    Yeast

    Mutation

    SEC66 transmembrane domain

    Promoter

    VAC8 promoter

    Available since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNA0304

    Plasmid

    #165612

    Purpose

    Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    NotI site replaced the CAN1 spacer in parent vect…

    Promoter

    SNR52

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKT0103

    Plasmid

    #8718

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    yEVenus
    

    Use

    Tags

    Codon optimized linker

    Expression

    Yeast

    Mutation

    yEGFP with F46L, S65G, V68L, S72A, M153T, V163A, …

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYC52

    Plasmid

    #63908

    Purpose

    Recipient integrative vector to construct CFP tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Use

    Tags

    CFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GTH-r

    Plasmid

    #81106

    Purpose

    Gene Tagging vector HIS/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    mCherry
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJK-caCas9-NatMX-Neut5L-Leu2 drive

    Plasmid

    #89578

    Purpose

    caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus

    Depositor

    George Church

    Article

    Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

    Insert

    Leu2 gene drive
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRS416 Gal TDP43 Q331K

    Plasmid

    #27461

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Glutamine 331 mutated to Lysine (Q331K)

    Promoter

    Available since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

23. pGAD424-hSyntenin2b

    Plasmid

    #89427

    Purpose

    Two-hybrid system

    Depositor

    Fritz Rathjen

    Article

    Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

    Insert

    SDCBP2 (SDCBP2 Human)
    

    Use

    Tags

    Gal4 AD

    Expression

    Yeast

    Mutation

    *see below

    Promoter

    ADH1

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAV0771

    Plasmid

    #133513

    Purpose

    Expression of mTagBFP2 tagged CHD domain to monitor actin cytoskeleton

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pAde6(PmeI)-p(act1)-3mTagBFP2-CHDRng2-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. M4757 KanMX::TRP1 Disruptor Converter

    Plasmid

    #51687

    Purpose

    Yeast marker swap plasmid for converting KanMX3 to TRP1

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    TRP1 (TRP1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pI4-MTU-DO-URA

    Plasmid

    #160025

    Purpose

    Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Uracil selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMTU-DO-HIS

    Plasmid

    #160005

    Purpose

    Low-copy expression vector for K. marxianus, histidine selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJT43_GalL_BE-P(AP)3

    Plasmid

    #145036

    Purpose

    Expresses BE-P(AP)3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    BE-(AP)3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAG414GAL-ccdB-EGFP

    Plasmid

    #14191

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAG414GPD-GaCYP722C(pYL777)

    Plasmid

    #178290

    Purpose

    Expresses GaCYP722C from G. arboreum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-GaCYP722C-TCYC1

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Insert

    GaCYP722C
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAP

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pXP414

    Plasmid

    #26841

    Depositor

    Nancy DaSilva, Suzanne Sandmeyer

    Article

    Fang et al Yeast. 2010 Sep 10. ():.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGK415-­rtetTAK8N, L131L

    Plasmid

    #165969

    Purpose

    Expresses rTetTA (K8N, L131L) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    tetR
    

    Use

    Synthetic Biology

    Tags

    three copies of VP16 (VP48)

    Expression

    Yeast

    Mutation

    Promoter

    pPGK1

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCfB2374

    Plasmid

    #67534

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae XI-1 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pIYC05

    Plasmid

    #64742

    Purpose

    Yeast expression plasmid: acsL641P, ALD6

    Depositor

    Jens Nielsen

    Article

    Chen et al Metab Eng. 2013 Jan;15:48-54. doi: 10.10

    Insert

    acsL641P, ALD6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

35. pCS2+ cMyc Dam_f_GFPoNLS

    Plasmid

    #85820

    Purpose

    iDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    cMyc Dam_f_GFPoNLS
    

    Use

    Tags

    cMyc and oNLS (optimized nuclear localization sig…

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    L122A.

    Promoter

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. p415 Gcs1

    Plasmid

    #112646

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Gcs1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Met25

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pPTK018-3b-RFP_Sec

    Plasmid

    #84977

    Purpose

    Type 3b, Red fluorescent protein

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    Red fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    BsaI site removed (1677 c to g and 1794 a to g)

    Promoter

    Non

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHCas9-Nours

    Plasmid

    #107733

    Purpose

    Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

    Depositor

    Sheng Yang

    Article

    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Human optimized

    Promoter

    TEF1

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDS250

    Plasmid

    #34969

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    V416I

    Promoter

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJZC583

    Plasmid

    #62314

    Purpose

    sgRNA with 2x MS2 for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 2x MS2 binding module
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge