7,935 results

1. pAG305GAL-EGFP-ccdB

   Plasmid

   #14305

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAG413GPD-ccdB-ECFP

   Plasmid

   #14166

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   ECFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPICZc-Ub-R-HsACTB*-thymosinB-8His

   Plasmid

   #111149

   Purpose

   Expresses arginylated human beta-actin fused with thymosin beta and a His tag.

   Depositor

   Mohan Balasubramanian

   Article

   Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

   Insert

   ACTB (ACTB Human)
   

   Use

   Pichia pastoris integration plasmid

   Tags

   Thymosin beta and a His-tag

   Expression

   Yeast

   Mutation

   N-terminal arginylation

   Promoter

   AOX1

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTSK450

   Plasmid

   #34552

   Depositor

   James McNally

   Insert

   MS2-CP-NLS
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Met25

   Available since

   Jan. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRS416-PHO5-GFP-Osh3

   Plasmid

   #58839

   Purpose

   Expresses GFP-Tagged Osh3 in yeast

   Depositor

   Tim Levine, Sean Munro

   Article

   Levine et al Mol Biol Cell. 2001 Jun;12(6):1633-44.

   Insert

   Osh3p (OSH3 Budding Yeast)
   

   Use

   Tags

   GFP and Myc

   Expression

   Yeast

   Mutation

   Promoter

   PHO5

   Available since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pSP568

   Plasmid

   #139493

   Purpose

   Insertion of the pSTL1 promoter and 24 MS2 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

   Depositor

   Serge Pelet

   Article

   Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

   Insert

   pSTL1 24xMS2sl
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   pSTL1

   Available since

   June 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pXP420 v2 (repaired Amp promoter)

   Plasmid

   #86920

   Purpose

   Shuttle vector to facilitate gene expression for metabolic engineering in S. cerevisiae. Contains TEF1 promoter and CYC1 terminator for insertion of gene of interest. Contains HIS3 selection marker.

   Depositor

   Gary Yellen

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIAeBlue

   Plasmid

   #185891

   Purpose

   In vivo gene amplification (HamAmp) of AeBlue expression module at RPL25 locus

   Depositor

   Claudia Vickers

   Insert

   PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>AeBlue>TPGK1- PBTS1> RPL25(partial; Arm2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFS119

   Plasmid

   #12379

   Depositor

   Nick Rhind

   Article

   Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

   Insert

   ade6, thymidine kinase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGM-ENO2-mCherry-YFP

    Plasmid

    #180570

    Purpose

    Dual fluorescent reporter for 5' UTR activity in yeast mCherry/YFP

    Depositor

    Joel McManus

    Article

    May et al Elife. 2023 May 25;12:e69611. doi: 10.75

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDRF1-GW AKAR3-EV-NR

    Plasmid

    #182534

    Purpose

    Expresses the non responsive PKA FRET sensor AKAR3-EV (T506A) in budding yeast

    Depositor

    Bas Teusink

    Article

    Botman et al FEMS Yeast Res. 2023 Jan 4;23:foad029. d

    Insert

    AKAR3-EV-NR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T506A mutation

    Promoter

    PMA1

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. E2-Crimson-FKBP(M)

    Plasmid

    #85002

    Purpose

    Expresses E2-Crimson-FKBP(M)

    Depositor

    Benjamin Glick

    Article

    Barrero et al Cell Logist. 2016 Jun 24;6(3):e1204848.

    Insert

    E2-Crimson-FKBP(M)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJW1665

    Plasmid

    #112039

    Purpose

    Integrating plasmid for expression of ER localized mCherry at TRP1 locus

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    ER-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pWS1837

    Plasmid

    #123058

    Purpose

    _-factor pheromone

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    _-factor [3-4a]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pESC-NAT-Ago-Dcr

    Plasmid

    #160381

    Purpose

    Overexpression of Ago-Dcr fusion protein in yeast

    Depositor

    John McCusker

    Insert

    Ago- Dcr
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRSCupGFP

    Plasmid

    #1086

    Depositor

    Susan Lindquist

    Article

    Glover et al Cell 1997 May 30;89(5):811-9.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pAG424GAL-ccdB-HA

    Plasmid

    #14247

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. E2-Crimson-FKBP(L,V)

    Plasmid

    #85000

    Purpose

    Expresses E2-Crimson-FKBP(L,V) in yeast

    Depositor

    Benjamin Glick

    Article

    Barrero et al Cell Logist. 2016 Jun 24;6(3):e1204848.

    Insert

    E2-Crimson-FKBP(LV)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAGT572_Nemo

    Plasmid

    #153487

    Purpose

    Level 1 episomal expression plasmid for S. cerevisiae

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPAP002

    Plasmid

    #153489

    Purpose

    Level 1 episomal expression plasmid for P. pastoris

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJZC523

    Plasmid

    #62320

    Purpose

    sgRNA for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCfB3039(XII-2 MarkerFree)

    Plasmid

    #73279

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-2 (Chr XII: 808805..809939)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p416PGK1-OsCYP711A2(pYL770)

    Plasmid

    #178301

    Purpose

    Expresses OsCYP711A2 from O. sativa in Saccharomyces cerevisiae. Centromeric URA, PPGK1-OsCYP711A2-Tpho5

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Insert

    OsCYP711A2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pYC50

    Plasmid

    #63906

    Purpose

    Recipient integrative vector to construct MYC tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Use

    Tags

    MYC

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDZ645 pKAN 1x-mCherry-12xPP7 V4

    Plasmid

    #73173

    Purpose

    Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    1x-mCherry
    

    Use

    Tags

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRG223MX

    Plasmid

    #64523

    Purpose

    Episomal E. coli/S. cerevisiae shuttle vector

    Depositor

    Joerg Stelling

    Article

    Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFA6a-kanMX-tetO-Pcyc1-3xFLAG-LINKER

    Plasmid

    #52689

    Purpose

    Same as 41022, with additional linker

    Depositor

    Michael Nick Boddy

    Insert

    tetO7-Pcyc1-3xFLAG+LINKER
    

    Use

    Promoter replacement

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCfB2797

    Plasmid

    #63639

    Purpose

    pCfB2797 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

    Depositor

    Irina Borodina, Jerome Maury

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. M4753 KanMX::ADE2 Disruptor Converter

    Plasmid

    #51683

    Purpose

    Yeast marker swap plasmid for converting KanMX3 to ADE2

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    ADE2 (ADE2 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGM-ENO2-YFP-mCherry

    Plasmid

    #180569

    Purpose

    Dual fluorescent reporter for 5' UTR activity in yeast YFP/mCherry

    Depositor

    Joel McManus

    Article

    May et al Elife. 2023 May 25;12:e69611. doi: 10.75

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAV0770

    Plasmid

    #133518

    Purpose

    Expression of mTagBFP2 tagged beta-tubulin to monitor microtubules

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pAde6(PmeI)-p(atb2)-mTagBFP2-atb2-terminator(atb2)-hphMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. BB3aZ_14*

    Plasmid

    #98542

    Purpose

    empty BB3 for insertion and overexpression of 1 gene in P. pastoris

    Depositor

    Brigitte Gasser, Diethard Mattanovich, Michael Sauer

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. LHP938 - Gal1-HA-Ub

    Plasmid

    #32175

    Depositor

    Linda Hicke

    Insert

    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Gal1

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

34. pAG424GPD-ccdB-HA

    Plasmid

    #14248

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBM5218

    Plasmid

    #18796

    Depositor

    Mark Johnston

    Article

    Wang et al Nat Protoc. 2008;3(10):1569-77. doi: 10.

    Insert

    Ty5-HIS3AI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 27, 2008

    Availability

    Academic Institutions and Nonprofits only

36. pXP742

    Plasmid

    #46060

    Depositor

    Nancy DaSilva

    Article

    Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH2

    Available since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAV0612

    Plasmid

    #133505

    Purpose

    Expression of 3xmTagBFP2 targeted to the plasma membrane with an amphipathic helix of RitC

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pAde6(PmeI)-p(tdh1)-3mTagBFP2-RitC-terminator(ScADH1)-bsdMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJAS1668

    Plasmid

    #158593

    Purpose

    VioE-ePTS1 CEN6/ARS4

    Depositor

    John Dueber

    Article

    Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

    Insert

    VioE-ePTS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p41Nat MagicFluor

    Plasmid

    #58563

    Purpose

    p41Nat carrying both mating type markers buffered by the Cyc1 terminator

    Depositor

    Leonid Kruglyak

    Insert

    Ste2p-Citrine, Cyc1 term, Ste3p-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRS415-Cas9-VPR

    Plasmid

    #163971

    Purpose

    Trifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeast

    Depositor

    Jiazhang Lian

    Article

    Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

    Insert

    Cas9-VPR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge