8,025 results
-
Plasmid#81105PurposeGene Tagging vector HIS/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)DepositorInsertmCitrine
UseCre/LoxTagsmCitrineExpressionYeastMutationMutated mCitrine for monomeric isoform (A206K L22…PromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUB23-L-beta-gal
Plasmid#8820DepositorInsertbeta-gal
UseTagsUbiquitinExpressionYeastMutationL (leucine) at amino terminus of beta-galPromoterAvailable sinceDec. 28, 2005AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3
Plasmid#65451PurposepILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.DepositorTypeEmpty backboneUseTagsyEGFP reporterExpressionYeastMutationPromoterAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pITGFP3
Plasmid#65452PurposepITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.DepositorTypeEmpty backboneUseTagsyEGFP-CLN2PEST reporterExpressionYeastMutationPromoterAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB8383
Plasmid#126917PurposePlasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of tHMG1DepositorInserttHMG1
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWS627
Plasmid#123064PurposeG___ bicistronic proteinDepositorInsertSte4-2A-Ste18 [3-4a]
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK372
Plasmid#73597PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-15 fatty acid desaturase
UseTagsFLAG tag and c-myc tagExpressionYeastMutationPromoterGAL1 and GAL10Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMEG_BB3_YL001
Plasmid#117825PurposeOverexpession plasmid of glycerol kinase (GUT1) in Y. lipolyticaDepositorInsertYlpTEF_YlGUT1_YlCyc1TT
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPS-CFP2-SpHis5
Plasmid#44853PurposeYeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with SpHis5 selectionDepositorInsertyoPS-CFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pXP732
Plasmid#46058DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterCUP1Available sinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHUM
Plasmid#64170Purposeharbours marker genes HIS3, URA3 and MET17DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW FLIPglu-600μΔ13V
Plasmid#28001DepositorInsertFLIPglu-600μΔ13V
UseTagseCFP and eYFPExpressionYeastMutationF16A in mglBPromoterAvailable sinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSJD001
Plasmid#158589PurposePex22(1-36)-mRuby2 peroxisome markerDepositorInsertPex22(1-36)-mRuby2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPS-CFP2-Kan
Plasmid#44948PurposeYeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with Kan selectionDepositorInsertyoPS-CFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pVW284
Plasmid#139494PurposeExpression vector for the PP7-GFP protein in S. cerevisiaeDepositorInsertpADH1 PP7∆FG-GFPenvy tCYC1
UseTagsExpressionYeastMutation∆FGPromoterpADH1Available sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVW300
Plasmid#139495PurposeExpression vector for the PP7-GFP protein in S. cerevisiaeDepositorInsertpTEF PP7∆FG-GFPenvy tCYC1
UseTagsExpressionYeastMutation∆FGPromoterpTEFAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG414GAL-ccdB-DsRed
Plasmid#14359DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastMutationPromoterAvailable sinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
AtCOPT1 upGFP
Plasmid#117899Purposeprotein expressionDepositorInsertAtCOPT1 upGFP (COPT1 Mustard Weed)
UseTagseGFPExpressionYeastMutationPromoterAOXAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046457
Plasmid#145424PurposeYeast Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046390
Plasmid#148996PurposeYeast Expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available sinceMay 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
-
LHP462 - Ub-HA
Plasmid#32169DepositorUseTagsHAExpressionYeastMutationPromoterCUP1Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
-
-
pR208IIM1
Plasmid#45352DepositorInsertUBR1 mutant
UseTagsExpressionYeastMutationP406S, DE317NKPromoterADHAvailable sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046332
Plasmid#145413PurposeYeast Expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
-
YCPlac33-sGFP-VRG4
Plasmid#25447DepositorInsertSynthetic Green Fluorescent Protein - VRG4
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
p42Nat 3-F GW
Plasmid#58557PurposeMulticopy with clonNat resistance marker for triple Gateway insertionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
EZ-L767
Plasmid#131773PurposePADH1_sFUS_Fusion Red_PixD_TACT1DepositorInsertPADH1_sFUS_Fusion Red_PixD_TACT1
UseIntegration into δ-sitesTagsExpressionYeastMutationPromoterPADH1Available sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pINER2R
Plasmid#185882PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (11 copy).DepositorInsertPRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B>RPL25(partial; Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDZ514 pURA MET25p PP7-PS-2x-yeGFP
Plasmid#72230PurposeUracil selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoterDepositorInsertPP7-PS-2x-yeGFP
UseTagsExpressionYeastMutationPromoterMET25Available sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3050(gRNA XII-5)
Plasmid#73292PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-5DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJZC625
Plasmid#62321PurposesgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme design for yeast cellsDepositorInsertsgRNA +1x MS2, Pol II promoter with ribozyme cleavage
UseTagsExpressionYeastMutationPromoterpAdhAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB2989
Plasmid#63636PurposepCfB2989 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoternoneAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXP822
Plasmid#46064DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterGAL1Available sinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLK89 - Edit-directing plasmid cloning insert
Plasmid#98846PurposeContains the insert used for step 2 of edit-directed plasmid cloningDepositorInsertKanR
UseCRISPRTagsExpressionYeastMutationChanged sequence to remove a MluI cut sitePromoterAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB1-Cterminal6xHis
Plasmid#60387PurposeExpression of yeast alpha tubulin (TUB1)- Cterminal 6xHis using a GAL promoterDepositorInsertTUB1 (TUB1 Budding Yeast)
UseTags6xHISExpressionYeastMutationPromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-bleMX6-Purg1-3FLAG
Plasmid#19357DepositorInserturg1 promoter
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX6-P41nmt1-3FLAG
Plasmid#19349DepositorInserthphMX6
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only