8,173 results
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Plasmid#27277DepositorAvailable SinceFeb. 1, 2011AvailabilityAcademic Institutions and Nonprofits only
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pRS403-PGAL1-weakSC_GFP
Plasmid#22315DepositorInsertGFP-rad9
UseIntegrating plasmidExpressionYeastAvailable SinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pXRhMX
Plasmid#63146PurposeHPH marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxExpressionBacterial and YeastAvailable SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAV0756
Plasmid#133508PurposeExpression of 3mCherry tagged CRIB domain to monitor active Cdc42DepositorInsertpHis5(StuI)-p(act1)-CRIB(gic2aa2-181)-3mCherry-bsdMX
ExpressionYeastAvailable SinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pH3F
Plasmid#85773PurposeN-ICE plasmid pH3FDepositorInsertloxP-HphMX-loxP-3FLAG
UseCre/LoxExpressionYeastAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ube2d2
Plasmid#31410DepositorAvailable SinceOct. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-3nmt1-mMaple3
Plasmid#105167PurposeYeast gene targetingDepositorInsertkanMX6-3nmt1-mMaple3
Tags3nmt1-mMaple3ExpressionBacterial and YeastPromoter3nmt1Available SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-3xHA-LINKER
Plasmid#52686PurposeSame as 41024, with additional linkerDepositorInserttetO7-Pcyc1-3xHA+LINKER
UsePromoter replacementExpressionYeastAvailable SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-APL5-iGFPx6
Plasmid#105252PurposePlasmid for pop-in/pop-out replacement of Apl5 with Apl5-iGFPx6.DepositorAvailable SinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRP1454
Plasmid#64510PurposeExpresses destabilized Citrine in yeast.DepositorInsertCitrine-ADH1-tail
ExpressionYeastPromoterTDH3Available SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
FRP1671
Plasmid#64538PurposeInducible MET15 geneDepositorAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
nuc-yHS1-M7A,H102A
Plasmid#159168PurposeNuclear labile heme reporterDepositorInsertnuc-yHS1-M7A,H102A
TagsSV40 NLSExpressionYeastMutationMutated both Met 7 and His 102 of the cyt b562 mo…PromoterGPDAvailable SinceSept. 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
mito-yHS1-M7A,H102A
Plasmid#159165PurposeMitochondrial labile heme reporterDepositorInsertmito-yHS1-M7A,H102A
TagsCOX4 pre-sequenceExpressionYeastMutationMutated both Met 7 and His 102 of the cyt b562 mo…PromoterGPDAvailable SinceSept. 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
HisSumoOphP
Plasmid#189672PurposeHisSUMO tagged OphP to express OphP in pichia pastorias.DepositorInsertHisSumoOphP
TagsHisSumo taggedExpressionYeastAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
GPD 104QDProGFP p416
Plasmid#15569DepositorInserthtt 104Q Delta Pro (HTT Human)
TagsGFPExpressionYeastMutationProline-rich region removedAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pNB777
Plasmid#60153PurposeRed click beetle luciferase (Promega) driven by LEU1 promoter.DepositorInsertCBR
ExpressionYeastPromoterLEU1prAvailable SinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-vps10-104
Plasmid#115426PurposeGenerate the vps10-104 allele via pop-in pop-out mutagenesisDepositorInsertvps10-104
ExpressionYeastMutationdomain 1 deletionPromoterEndogenousAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNC951
Plasmid#20400DepositorInsertCFP-reporter
ExpressionYeastMutationX=UbiY (see schematic) linker=deltakAvailable SinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pXRLy2
Plasmid#63149PurposeLys2 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxExpressionBacterial and YeastAvailable SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
LHP556 - UBC4
Plasmid#32440DepositorAvailable SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAG414GAL-EGFP-ccdB
Plasmid#14311DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-ccdB-EGFP
Plasmid#14192DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pZEN
Plasmid#129113PurposeZ. rouxii episomal vector containing ClonNAT resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZCAG
Plasmid#129114PurposeZ. rouxii centromeric vector containing G418 resistanceDepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV0764
Plasmid#133520PurposeExpression of mCherry targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)DepositorInsertpLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-mCherry-AHDL-bsdMX
ExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAH233
Plasmid#121144PurposeExpression of gRNA with BbsI placeholder for target cloningDepositorInsertgRNA cassette
UseCRISPRExpressionYeastPromoterS.pombe rrk1Available SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2
Plasmid#60388PurposeExpression of yeast beta tubulin (TUB2) using a GAL promoterDepositorInsertTUB2
ExpressionYeastPromoterGALAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
7859 pRS305-pUG72
Plasmid#10897DepositorAvailable SinceApril 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pXP122
Plasmid#26827DepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCfB2990
Plasmid#63645PurposepCfB2990 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastPromoternoneAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUDP044
Plasmid#101168PurposepUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)DepositorInsertpolycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
UseCRISPRExpressionYeastPromoterScTDH3Available SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPS2169
Plasmid#1364DepositorAvailable SinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJT10R
Plasmid#98307PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3AcNES1>TRPL41B
ExpressionYeastMutationERG20 N127WAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDIV490
Plasmid#177698PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRHR2p (Debaryomyces hansenii ) and TDH3p (Candida…Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKT0211
Plasmid#8734DepositorInsertyECitrine A206R
TagsCodon optimized linkerExpressionYeastMutationyEGFP with S65G, V68L, Q69M, S72A, T203Y, A206RAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pICE TRP1-KanMx
Plasmid#122902PurposeTRP1-KanMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pATP416-pluxO5-ptetO7-crtYBI
Plasmid#165975PurposeExpresses carotenoid biosynthesis gene in response to homoserine lactone and doxycycline in yeast expressing LuxTA and rtTADepositorInsertsXanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
BTS1 (BTS1 Budding Yeast)
UseSynthetic BiologyExpressionYeastMutationC1281A, G1698A and C66A, C1359TPromoterSynthetic promoter (pluxO5), Synthetic promoter (…Available SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGK415-phlTA2-1E
Plasmid#165970PurposeExpresses phlTA(2-1E) mutantDepositorInsertphlF
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…PromoterpPGK1 mutantAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
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GAL FLAG72QDProCFPp303
Plasmid#15586DepositorInserthtt 72Q Delta Pro (HTT Human)
TagsCFP and FLAGExpressionYeastMutationProline-rich region removedAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only