8,265 results
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Plasmid#251973PurposeYeast mtDNA editing with a mid-strength promoter for lower expression levelDepositorInsertABE8e
UseCRISPRTagsMTS and V5-P2A-EGFPExpressionYeastPromoterADH1Available SinceFeb. 24, 2026AvailabilityAcademic Institutions and Nonprofits only -
pPT24-mtArg8m-V5
Plasmid#251979PurposeFor biolistic transformation to express Arg8m from mtDNADepositorInsertmtArg8m
TagsV5ExpressionYeastAvailable SinceFeb. 24, 2026AvailabilityAcademic Institutions and Nonprofits only -
MTS-ABE-ARG8
Plasmid#251976PurposeExpression of ABE-ARG8 fusion protein in yeastDepositorInsertABE-ARG8
UseCRISPRTagsMTSExpressionYeastPromoterGPDAvailable SinceFeb. 24, 2026AvailabilityAcademic Institutions and Nonprofits only -
NLS-ABE8e-GFP
Plasmid#251972PurposeYeast nDNA editing with ABE8eDepositorInsertABE8e
UseCRISPRTags3xNLS-3xFLAG-EGFP and NLSExpressionYeastPromoterGPDAvailable SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pPT24-mtArg8m*-V5
Plasmid#251978PurposeFor biolistic transformation to express Arg8m* from mtDNADepositorInsertmtArg8m*
TagsV5ExpressionYeastMutationpremature stop CAA->TAA, Q79*Available SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pRS426-SNR52-crRNA
Plasmid#251984PurposeExpression of crRNA (version 3) for editing, used together with tracrRNA Plasmid #251985. This plasmid is also called crRNA in the paper.DepositorInsertcrRNA
UseCRISPRExpressionYeastPromoterSNR52Available SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pPT24-mtArg8m*-V5-UTR-HH-ntsgRNA-HDV
Plasmid#251982PurposeFor biolistic transformation to express Arg8m* and a non-targeting control sgRNA from mtDNADepositorInsertsmtArgm*
HH-nt sgRNA-HDV
TagsV5ExpressionYeastMutationpremature stop CAA->TAA, Q79*Available SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
nArg8-EGFP
Plasmid#251977PurposeExpression of nuclear ARG8-EGFP as controlDepositorInsertnArg8-EGFP
TagsEGFPExpressionYeastPromoterGPDAvailable SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pRembiv
Plasmid#252216PurposeYeast expression of BK polyomavirus VP1 and aeBlue reporterDepositorInsertsBKV-IV VP1
aeBlue
Formaldehyde dehydrogenase 1
ExpressionYeastPromoterFDH1 and MAL32Available SinceFeb. 23, 2026AvailabilityIndustry, Academic Institutions, and Nonprofits -
HRPEadh-pAG303
Plasmid#249735PurposeDerived from pAG303GPD-ccdb (#14134); GPD replaced with a synthetic 5xHRPE-ADHutr sequence for orthogonal activation by plant RAP2.12-based TFs.DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
SYRAP_pDONR201
Plasmid#249814PurposeEncodes SYRAP (RAP2.12-GAL4STE12), Gateway compatible as Donor plasmidDepositorInsertRAP2.12-GAL4STE (RAP2.12 Budding Yeast, Mustard Weed)
UseSynthetic BiologyTagsGAL4-STE12ExpressionPlant and YeastAvailable SinceFeb. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
HRPEadh-pAG305
Plasmid#249734PurposeDerived from pAG305GPD-ccdb (#14138); GPD replaced with a synthetic 5xHRPE-ADHutr sequence for orthogonal activation by plant RAP2.12-based TFs.DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-GFP-PHO8 K(24,25,30,33)R
Plasmid#207044PurposeExpression of GFP-Pho8 point mutant under CUP1 promoter.DepositorInsertPHO8
TagsGFPExpressionYeastMutationK(24,25,30,33)RPromoterpCUP1Available SinceFeb. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mIAA7-mCherry-kanNP1
Plasmid#247621PurposeYeast gene tagging with mIAA7DepositorInsertmIAA7
TagsmCherryExpressionYeastAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSC6
Plasmid#233020PurposeInducible Conjugative PlasmidDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAG304-ccdbNLUC
Plasmid#249736PurposeDerived from pAG304GPD-ccdb (#14136); GPD removed and NLUC cds inserted downstream gateway cassette. Useful for cloning promoter-NLUC fusions in Saccharomyces cerevisiaeDepositorTypeEmpty backboneExpressionYeastAvailable SinceJan. 28, 2026AvailabilityAcademic Institutions and Nonprofits only -
UbSYRAP_pDONR201
Plasmid#249816PurposeEncodes UbSYRAP, a SYRAP split version (UBQ-RAP2.12-GAL4STE12), Gateway compatible as Donor plasmidDepositorInsertUBQ-RAP2.12-GAL4STE (RAP2.12 Budding Yeast, Mustard Weed)
UseSynthetic BiologyTagsGAL4-STE12 and UBQExpressionPlant and YeastAvailable SinceJan. 28, 2026AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-Sec7-4V5-APEX2-URA
Plasmid#207060PurposeExpressing Sec7-4V5-APEX2. Insufficient signal for EM.DepositorInsertSec7
Tags4xV5-APEX2ExpressionYeastPromoterpSEC7Available SinceJan. 27, 2026AvailabilityAcademic Institutions and Nonprofits only -
P1K-3HA-Atg8-MET15
Plasmid#207032PurposeExpression of 3HA-Atg8.DepositorInsertATG8
Tags3xHAExpressionYeastPromoterpATG8Available SinceJan. 27, 2026AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8(T56A T87A)-MET15
Plasmid#207048PurposeExpression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
Tags3xVSVExpressionYeastMutationT56A T87AAvailable SinceJan. 27, 2026AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8(P85A P86A)-MET15
Plasmid#207049PurposeExpression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
Tags3xVSVExpressionYeastMutationP85A P86AAvailable SinceJan. 27, 2026AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-Snf7-4V5-APEX2-URA
Plasmid#207058PurposeExpressing Snf7-4V5-APEX2. Insufficient signal for EM.DepositorInsertSnf7
Tags4xV5-APEX2ExpressionYeastPromoterpSNF7Available SinceJan. 27, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAvA143
Plasmid#248147Purposemutated LuxR transcriptional activator for expression in yeast: fused with Gal4 activation domain with 'gen2' mutationsDepositorInsertluxR (gen3 mutations)
UseIntegration vectorTagsGal4 activation domain and nuclear localization s…ExpressionYeastMutationGal4_AD: F51 (TTC→TTT), G88E. LuxR: K53E, N86Y, I…PromoterpPGK1Available SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAvA141
Plasmid#248146Purposemutated LuxR transcriptional activator for expression in yeast: fused with Gal4 activation domain with 'gen2' mutationsDepositorInsertluxR (gen2 mutations)
UseIntegration vectorTagsGal4 activation domain and nuclear localization s…ExpressionYeastMutationGal4_AD: G8V. NLS: K120N. LuxR: N86K, K104E, M135VPromoterpPGK1Available SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAvA139
Plasmid#248145Purposemutated LuxR transcriptional activator for expression in yeast: fused with Gal4 activation domain with 'gen1' mutationsDepositorInsertluxR (gen1 mutations)
UseIntegration vectorTagsGal4 activation domain and nuclear localization s…ExpressionYeastMutationGal4_AD: N24K, P41 (CCA→CCG), N46D, T92S, V98 (GT…PromoterpPGK1Available SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
P2802
Plasmid#248828PurposeAll repressors for the WTC847 system.DepositorInsertLexA-L7-hER(L387M), LexA-hER-adh1tail
ExpressionYeastMutationLexA-L7-hER: L387MPromoterpACT1 (LexA-L7-hER(L387M)), P7SulA.1(LexA-hER-adh…Available SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
P2814
Plasmid#248829PurposeAll repressors for the WTC848 system.DepositorInsertLacI(6m), LacI(5m)
TagsSV40NLS on LacI(6m)ExpressionYeastMutationLacI(6m): T167A, W220F, M254L, V119I, Q60G, M242L…PromoterpRnr2 (LacI(6m), P4Lacn.2 (LacI(5m))Available SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
P2880
Plasmid#248830PurposeAll repressors for the WTC848 system.DepositorInsertLacI(6m), LacI(5m)
TagsSV40NLS on LacI(6m)ExpressionYeastMutationLacI(6m): T167A, W220F, M254L, V119I, Q60G, M242L…PromoterpRnr2 (LacI(6m), P4Lacn.2 (LacI(5m))Available SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
P2881
Plasmid#248831PurposeAll repressors for the WTC848 system.DepositorInsertLacI(6m), LacI(5m)
TagsSV40NLS on LacI(6m)ExpressionYeastMutationLacI(6m): T167A, W220F, M254L, V119I, Q60G, M242L…PromoterpRnr2 (LacI(6m), P4Lacn.2 (LacI(5m))Available SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
P2072
Plasmid#248832PurposeLac12 for IPTG importDepositorInsertLac12
ExpressionYeastPromoterpACT1Available SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
P2882
Plasmid#248833PurposeLac12 for IPTG importDepositorInsertLac12
ExpressionYeastPromoterpACT1Available SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
P2883
Plasmid#248834PurposeLac12 for IPTG importDepositorInsertLac12
ExpressionYeastPromoterpACT1Available SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
P2884
Plasmid#248835PurposeLac12 for IPTG importDepositorInsertLac12
ExpressionYeastPromoterpACT1Available SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
P2885
Plasmid#248836PurposeLac12 for IPTG importDepositorInsertLac12
ExpressionYeastPromoterpACT1Available SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
P2754
Plasmid#248837PurposeP7SulA.1 tagging casette for WTC847 with Nourseothricin resistanceDepositorInsertP7SulA.1
ExpressionYeastAvailable SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
P2755
Plasmid#248838PurposeP7SulA.1 tagging casette for WTC847 with Hygromycin resistanceDepositorInsertP7SulA.1
ExpressionYeastAvailable SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
P2765
Plasmid#248839PurposeP4Lacn.2 tagging casette for WTC848 with Nourseothricin resistanceDepositorInsertP4Lacn.2
ExpressionYeastAvailable SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
P2879
Plasmid#248840PurposeP4Lacn.2 tagging casette for WTC848 with Hygromycin resistanceDepositorInsertP4Lacn.2
ExpressionYeastAvailable SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
pPtGE31_ΔPtR
Plasmid#236260PurposeA version of the episome pPtGE31 with a deleted nonessential region, contains the NAT cassetteDepositorTypeEmpty backboneUseSynthetic Biology; P. tricornutum expressionExpressionBacterial and YeastAvailable SinceJan. 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
pPtGE31_ShBle
Plasmid#236261PurposeA version of the episome pPtGE31_ΔPtR with an added ShBle cassetteDepositorTypeEmpty backboneUseSynthetic Biology; P. tricornutum expressionExpressionBacterial and YeastAvailable SinceJan. 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
pPtGE30
Plasmid#236256PurposeTransmissible episome that contains the ShBle cassetteDepositorTypeEmpty backboneUseSynthetic Biology; P. tricornutum expressionExpressionBacterial and YeastAvailable SinceJan. 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
pPtGE31
Plasmid#236257PurposeTransmissible episome that contains the NAT cassetteDepositorTypeEmpty backboneUseSynthetic Biology; P. tricornutum expressionExpressionBacterial and YeastAvailable SinceJan. 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCBS-5740
Plasmid#249327PurposeExpresses sgRNA targeting the fcy gene in S. cerevisiaeDepositorInsertsgRNA targeting fcy
ExpressionYeastMutationN/AAvailable SinceJan. 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCBS-5738
Plasmid#249324PurposeTo express the EPEC DarT2(DLAA)-nScCas9(D10A) fusion protein in experiments for Append EditingDepositorInsertEPEC DarT2(DLAA)-nScCas9(D10A)
ExpressionYeastMutationDarT2(G49D, M86L, R92A, R193A) and nScCas9(D10A)Available SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCBS-5753
Plasmid#249325PurposeTo express the EPEC dDarT2(DLAA)-nScCas9(D10A) fusion protein in experiments for Append EditingDepositorInsertEPEC dDarT2(DLAA)-nScCas9(D10A)
ExpressionYeastMutationDarT2(G49D, M86L, R92A, E,170A, R193A) and nScCas…Available SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCBS-5737
Plasmid#249326PurposeExpresses Non-targeting sgRNADepositorInsertnontargeting sgRNA
ExpressionYeastMutationN/AAvailable SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pDEST-GBKT7-GNAI1
Plasmid#248653PurposeYeast 2-hybrid vector encoding human heterotrimeric G protein GNAI1 fused to the GAL4 DNA binding domain.DepositorAvailable SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pDEST-GBKT7-GNAO1
Plasmid#248654PurposeYeast 2-hybrid vector encoding human heterotrimeric G protein GNAO1 fused to the GAL4 DNA binding domain.DepositorAvailable SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pDEST-GBKT7
Plasmid#248655PurposeEmpty vector for generating yeast 2-hybrid GAL4 DNA binding domain fusion constructs by Gateway cloning. It may also be used as an empty vector experimental control.DepositorTypeEmpty backboneUseYeast 2-hybridTagsGAL4 binding domain, MycExpressionYeastAvailable SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pDEST-GADT7
Plasmid#248657PurposeEmpty vector for generating yeast 2-hybrid GAL4 activation domain fusion constructs by Gateway cloning. It may also be used as an empty vector experimental control.DepositorTypeEmpty backboneUseYeast 2-hybridTagsGAL4 activation domain, HAExpressionYeastAvailable SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only