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Plasmid#109058Purposeyeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.DepositorInsertHIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)
UseCre/LoxTagsExpressionBacterial and YeastMutationPromoterHIS3Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBCSK_LEU2_I
Plasmid#109060Purposeyeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites upstream of LEU2. For teaching purposesDepositorInsertLEU2 (LEU2 Zygosaccharomyces rouxii, Budding Yeast)
UseCre/LoxTagsExpressionBacterial and YeastMutationPromoterLEU2Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRP1864
Plasmid#159307PurposeYeast tagging vector to create gene fusion with yeast codon optimized tdKOkappa under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRP272
Plasmid#159305PurposeYeast tagging vector to create gene fusion with sfGFP under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-ODPAMTS (12)
Plasmid#101694PurposeODPAMTS in domestication vectorDepositorInsertCCAT-[MTS from S. cerevisiae Pyruvate dehydrogenase E1 component subunit alpha (YER178W)]-AATG
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-ODPBMTS (12)
Plasmid#101695PurposeODPBMTS in domestication vectorDepositorInsertCCAT-[MTS from S. cerevisiae Pyruvate dehydrogenase E1 component subunit beta (YBR221C)]-AATG
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pREP3xN_ccdB
Plasmid#79021PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, LEU2 selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG425GAL-ccdB-ECFP
Plasmid#14177DepositorTypeEmpty backboneUseGateway destinationTagsECFPExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSP303 pRS306 pRPS2-Far1DS-NLS-mCherry
Plasmid#68279PurposeSKARS: Fus3 MAPK activity sensor: Far1DS 2xNLS mCherryDepositorInsertFar1DS NLS mCherry
UseTagsmCherryExpressionYeastMutationPromoterRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCH044
Plasmid#47950PurposertTA under the control of GAL2 promoterDepositorInsertrtTA
UseTet inducible expressionTagsExpressionYeastMutationPromoterGal2Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNC952
Plasmid#20401DepositorInsertCFP-reporter
UseTagsExpressionYeastMutationX=UbiM (see schematic) linker=deltakPromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
Osa-pCAMBIA-1301-STTM408-3p
Plasmid#84217PurposeDisturb the expression of miR408-3p in riceDepositorInsertmiR408-3p
UseTagsExpressionBacterial, Plant, and Yeast…MutationPromoter2x35SAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV0784
Plasmid#133475PurposeIntegration into fission yeast genome at his5 locus with bsdMX selection cassetteDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICE ADE2-HIS3
Plasmid#122883PurposeADE2-HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-chNfasc (+RSLE)
Plasmid#89418PurposeTwo-hybrid systemDepositorInsertNFASC (NFASC Chicken)
UseTagsGAL4 DBDExpressionYeastMutationPromoterADH1Available sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTH730-staRLuc
Plasmid#38226DepositorInsertRenilla Luciferase
UseTagsExpressionYeastMutationPromoterTDH3 (=GPD)Available sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046170
Plasmid#149119PurposeYeast Expression plasmid for SARS-CoV-2 nsp10DepositorInsertSARS-CoV-2 nsp10 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
GTL-y
Plasmid#81100PurposeGene Tagging vector LEU/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)DepositorInsertmCitrine
UseCre/LoxTagsmCitrineExpressionYeastMutationMutated mCitrine for monomeric isoform (A206K L22…PromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC_delta ald1
Plasmid#126708Purposevector for knocking out ald1 in Komagataella phaffiiDepositorInsertHIS4
UseTagsExpressionYeastMutationPromoterHisAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046247
Plasmid#149124PurposeYeast Expression plasmid for SARS-CoV-2 nsp2DepositorInsertSARS-CoV-2 nsp2 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationwtPromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCGLex
Plasmid#17264DepositorTypeEmpty backboneUseTagsLexAExpressionYeastMutationPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
GTU-r
Plasmid#81096PurposeGene Tagging vector URA/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)DepositorInsertmCherry
UseCre/LoxTagsmCherryExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046415
Plasmid#149035PurposeYeast Expression plasmid for SARS-CoV-2 helicaseDepositorInsertSARS-CoV-2 helicase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046246
Plasmid#145553PurposeYeast Expression plasmid for SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046455
Plasmid#145487PurposeYeast Expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046231
Plasmid#145451PurposeYeast Expression plasmid for SARS-CoV-2 nsp8DepositorInsertSARS-CoV-2 nsp8 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-kanMX-tetO-Pcyc1-LINKER
Plasmid#52685PurposeSame as 41023, with additional linkerDepositorInserttetO7-Pcyc1+LINKER
UsePromoter replacementTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
Gal-Rad51 KR
Plasmid#33232DepositorInsertRad51 (RAD51 Budding Yeast)
UseTagsExpressionYeastMutationK191RPromoterGal1Available sinceFeb. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
YEplac181-GAL11
Plasmid#8628DepositorInsertGAL11 (GAL11 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationWild typePromoterAvailable sinceNov. 15, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJT79_GalL_nCDA1Δ192-BE3
Plasmid#145045PurposeExpresses nCDA1Δ192-BE3 in yeast cellsDepositorInsertnCDA1Δ192-BE3
UseCRISPRTagsExpressionYeastMutationpmCDA1(1-192AA); spCas9(D10A)PromoterGalLAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
KAP123-FLU (116)
Plasmid#24048DepositorInsertKAP123 (KAP123 Budding Yeast)
UseTagsFLUExpressionYeastMutationPromoterAvailable sinceMarch 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMZ285
Plasmid#52226PurposeExpression of sgRNA precursor targeted against ade6M210DepositorInsertrrk1-sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterrrk1Available sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCGLex/Zeo
Plasmid#17259DepositorTypeEmpty backboneUseTagsLexAExpressionYeastMutationPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-ECFP-ccdB
Plasmid#14300DepositorTypeEmpty backboneUseGateway destinationTagsECFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-ccdB-TAP
Plasmid#14264DepositorTypeEmpty backboneUseGateway destinationTagsTAPExpressionYeastMutationPromoterAvailable sinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS306Sup35R1+2
Plasmid#17716DepositorInsertSup35R1+2 (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLU
Plasmid#64174Purposeharbours marker genes LEU2 and URA3DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pED73 pRS304 pRPS2-SteDS-NLS-mCitrine
Plasmid#68278PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCitrineDepositorInsertSte7DS NLS mCitrine
UseTagsmCitrineExpressionYeastMutationPromoterpRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-X3
Plasmid#115568PurposeSynthetic acetylation substrate consisting of GFP fusion with 3 repeats of the Gcn5 consensus motifDepositorInsertGreen Fluorescent Protein
UseTagsExpressionBacterial and YeastMutationThree repeats of the Gcn5 consensus motif are tag…PromoterADH1Available sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV0587
Plasmid#133474PurposeIntegration into fission yeast genome at his5 locus with bleMX selection cassetteDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only