8,043 results

1. LD-BS-Trp1

   Plasmid

   #69503

   Purpose

   Improved version for complementation of yeast auxotrophic marker TRP1

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   TRP1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p413GPD-hTPI Ile170Val

   Plasmid

   #50720

   Purpose

   expression of the human TPI Ile170Val allele in yeast

   Depositor

   Markus Ralser

   Article

   Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

   Insert

   Triosephosphate isomerase 1 (TPI1 Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Isoleucine 170 to Valine

   Promoter

   GPD

   Available since

   Feb. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRS416 Gal-RNQ1 1-153-YFP

   Plasmid

   #18690

   Depositor

   Susan Lindquist

   Article

   Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

   Insert

   Rnq1 1-153
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   NonPrion domain of Rnq1

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pPS2192

   Plasmid

   #8923

   Depositor

   Pamela Silver

   Article

   Hood et al J Cell Sci 2001 Feb;114(Pt 3):589-97.

   Insert

   Clb2-deltaNLS-GFP (CLB2 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Deletion of CLB2 bipartite NLS (aa 183-200), KpnI…

   Promoter

   Available since

   Aug. 19, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGBW-m4046374

   Plasmid

   #149120

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pGBW-m4046372

   Plasmid

   #149048

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 helicase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 helicase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pGBW-m4046194

   Plasmid

   #148979

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF7a protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF7a protein (ORF7a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pGBW-m4046521

   Plasmid

   #145473

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp11

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp11 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pCfB2198

   Plasmid

   #67554

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XII-4 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pILGFP84

    Plasmid

    #83542

    Purpose

    Used to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    SSA1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SSA1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Gal4pBS34-Pleum

    Plasmid

    #60334

    Purpose

    Contains a promoter of two gal binding sites coupled wiht a core promoter derived from native Leucine promoter.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 11, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pGBW-m4046696

    Plasmid

    #153882

    Purpose

    Yeast expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pAG424GPD-ccdB-Cerulean

    Plasmid

    #14392

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSP571

    Plasmid

    #139498

    Purpose

    CRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNA

    Depositor

    Serge Pelet

    Article

    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

    Insert

    pGPD Cas9 / sgRNA (STL162)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    pGPD

    Available since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPS2700

    Plasmid

    #8924

    Depositor

    Pamela Silver

    Article

    Lei et al Genes Dev. 2001 Jul 15. 15(14):1771-82.

    Insert

    YRA1 (YRA1 Budding Yeast)
    

    Use

    Tags

    3x Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 19, 2005

    Availability

    Academic Institutions and Nonprofits only

16. pJZC548

    Plasmid

    #62316

    Purpose

    sgRNA with 1x PP7 for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 1x PP7
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTH399-SUP45-S30P

    Plasmid

    #29372

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    S30P mutant gene (corresponding to the sl23ts all…

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pTH375-SUP45-P174Q

    Plasmid

    #29383

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    P174Q mutant gene including genomic sequence 1432…

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pCfB2992

    Plasmid

    #63642

    Purpose

    pCfB2992 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

    Depositor

    Irina Borodina, Jerome Maury

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAG306GAL-EYFP-ccdB

    Plasmid

    #14331

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCfB2228

    Plasmid

    #67540

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-4 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLexA::nud-1

    Plasmid

    #11339

    Depositor

    Guy Caldwell

    Insert

    nud-1 (nud-1 Nematode)
    

    Use

    Tags

    LexA DNA Binding Domain

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

23. pLexA::lis-1

    Plasmid

    #11338

    Depositor

    Guy Caldwell

    Insert

    lis-1 (lis-1 Nematode)
    

    Use

    Tags

    LexA DNA Binding Domain

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

24. pAG426GAL-ccdB-ECFP

    Plasmid

    #14179

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRG217

    Plasmid

    #64531

    Purpose

    Centromeric E. coli/S. cerevisiae shuttle vector

    Depositor

    Joerg Stelling

    Article

    Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pICE TRP1-URA3

    Plasmid

    #122894

    Purpose

    TRP1-URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pICE LYS2-HphMx

    Plasmid

    #122898

    Purpose

    LYS2-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pICE LYS2-KanMx

    Plasmid

    #122899

    Purpose

    LYS2-KanMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pICE LYS2-NatMx

    Plasmid

    #122900

    Purpose

    LYS2-NatMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pICE TRP1-HphMx

    Plasmid

    #122901

    Purpose

    TRP1-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p2HGAL/GR/CYC

    Plasmid

    #1220

    Depositor

    Susan Lindquist

    Article

    Nathan et al Mol Cell Biol 1995 Jul;15(7):3917-25.

    Insert

    GR (Nr3c1 Rat)
    

    Use

    2micron

    Tags

    Expression

    Yeast

    Mutation

    pRS423 with GAL promoter and CYC terminator, 2mic…

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

32. pNX3a-PK6

    Plasmid

    #78355

    Purpose

    fission yeast epitope tagging

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    6xPK
    kanMX6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1*

    Plasmid

    #126739

    Purpose

    yeast plasmid overexpressing ADH2,ALD6 and ACS1 from Saccharomyces cerevisiae

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    adh2, acs1*, ald6
    

    Use

    Tags

    6xHis,Myc

    Expression

    Yeast

    Mutation

    T3542C and A30S, S629N (please see depositor comm…

    Promoter

    GAP

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAG423GPD-ccdB-ECFP

    Plasmid

    #14174

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDUAL-P41nmt1-Nup124-GFP-MTn

    Plasmid

    #154365

    Purpose

    Yeast expression of Nup124

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    Nup124 (nup124 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    See depositor comments below

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFA6a-6xGLY-HSV-kanMX6

    Plasmid

    #20775

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pPS1303

    Plasmid

    #8849

    Depositor

    Pamela Silver

    Article

    Shen et al Genes Dev 1998 Mar 1;12(5):679-91.

    Insert

    Gal-sGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

38. pRS413-IVb

    Plasmid

    #31459

    Depositor

    Ana Kitazono

    Article

    Kitazono et al Gene. 2011 Sep 15;484(1-2):86-9. doi: 10

    Insert

    linker
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

39. Ube2v3

    Plasmid

    #31431

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UEVLD Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

40. pMJ22

    Plasmid

    #63919

    Purpose

    Initial replicative vector

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Use

    Tags

    NATMX

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge