8,043 results

1. p413TEF-NYFP

   Plasmid

   #99557

   Purpose

   Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

   Depositor

   Liming Li

   Article

   Du et al Mol Microbiol. 2017 Apr;104(1):105-124.

   Insert

   Swi1 N-rich region (AA1-327) (SWI1 Budding Yeast)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Aug. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. BB3rN_AH

   Plasmid

   #98556

   Purpose

   empty BB3 for insertion and overexpression of 7 genes in P. pastoris

   Depositor

   Michael Sauer, Brigitte Gasser, Diethard Mattanovich

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pL-rpb1-C67Y

   Plasmid

   #10982

   Depositor

   Jeff Strathern

   Article

   Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

   Insert

   rpb1-C67Y (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Mutation 200 G to A in the RPB1 ORF resulting i…

   Promoter

   Available since

   Dec. 16, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pL-rpb1-C70Y

   Plasmid

   #10983

   Depositor

   Jeff Strathern

   Article

   Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

   Insert

   rpb1-C70Y (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Mutation 209 G to A in the RPB1 ORF resulting i…

   Promoter

   Available since

   Dec. 16, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pL-rpb1-H80Y

   Plasmid

   #10984

   Depositor

   Jeff Strathern

   Article

   Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

   Insert

   rpb1-H80Y (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Mutation 238 C to T in the RPB1 ORF resulting i…

   Promoter

   Available since

   Dec. 16, 2005

   Availability

   Academic Institutions and Nonprofits only

6. pHK

   Plasmid

   #64187

   Purpose

   harbours marker genes HIS3 and LYS2

   Depositor

   Markus Ralser

   Article

   Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPS1358

   Plasmid

   #8918

   Depositor

   Pamela Silver

   Article

   Kessler et al Genes Dev. 1997 Oct 1. 11(19):2545-56.

   Insert

   HRP1 (HRP1 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 19, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG304GAL-ccdB-EYFP

   Plasmid

   #14207

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EYFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAH3

   Plasmid

   #41827

   Purpose

   Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for phleomycin resistance.

   Depositor

   Melissa Moore

   Article

   Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

   Type

   Empty backbone

   Use

   Pcr template

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRB2963

    Plasmid

    #8678

    Depositor

    David Botstein

    Article

    Anders et al Mol Biol Cell 2001 Dec;12(12):3973-86.

    Insert

    GFP::TUB1-828 under GAL1 promoter (TUB1 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 30, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pNB787

    Plasmid

    #60152

    Purpose

    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus-PEST
    

    Use

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    LEU1pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p413GPD-hTPI Lys13Arg

    Plasmid

    #50722

    Purpose

    expression of the human TPI Lys13Arg allele in yeast

    Depositor

    Markus Ralser

    Article

    Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

    Insert

    Triosephosphate isomerase 1 (TPI1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    changed Lysine 13 to Argenine

    Promoter

    GPD

    Available since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p413GPD-hTPI Ile170Thr

    Plasmid

    #50721

    Purpose

    expression of the human TPI Ile170Thr allele in yeast

    Depositor

    Markus Ralser

    Article

    Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

    Insert

    Triosephosphate isomerase 1 (TPI1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Isoleucine 170 to Threonine

    Promoter

    GPD

    Available since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCfB4780

    Plasmid

    #106143

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pWHI2-NAT

    Plasmid

    #80588

    Purpose

    CEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoter

    Depositor

    John McCusker

    Article

    Vijayraghavan et al Genetics. 2018 Nov 29. pii: genetics.118

    Insert

    WHI2 (WHI2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Native WHI2 promoter

    Available since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pXP711

    Plasmid

    #46053

    Depositor

    Nancy DaSilva

    Article

    Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1

    Available since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pXP712

    Plasmid

    #46054

    Depositor

    Nancy DaSilva

    Article

    Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1

    Available since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. sup35-C653R

    Plasmid

    #1198

    Depositor

    Susan Lindquist

    Article

    True et al Nature 2004 Sep 9;431(7005):184-7. Epub

    Insert

    Sup35p (SUP35 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    changed cys 653 to arg

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pTH743-CEN-RLuc/slowstaCFLuc

    Plasmid

    #38223

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The last three codons of the full-length Firefly …

    Promoter

    ADH1 and TDH3 (=GPD)

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTH742-CEN-RLuc/slowminCFLuc

    Plasmid

    #38222

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3 (=GPD)

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJT3

    Plasmid

    #98304

    Purpose

    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of CUP1 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    TRPL3AcNES1>TRPL41B
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAG416GPD-Cerulean-YPT52

    Plasmid

    #18843

    Depositor

    Susan Lindquist

    Article

    Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    YPT52 (YPT52 Budding Yeast)
    

    Use

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

23. L1-YA

    Plasmid

    #15525

    Depositor

    Vance Lemmon

    Article

    Cheng et al J Neurochem. 2005 Aug . 94(4):1102-10.

    Insert

    L1-cytoplasmic domain-Y1176A (L1CAM Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Tyrosine 1176 changed to Alanine

    Promoter

    Available since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

24. pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherry

    Plasmid

    #68280

    Purpose

    SKARS: Mpk1 MAPK activity sensor: Mkk2DS (1-100) 2xNLS mCherry

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Mkk2DS NLS mCherry (MKK2 Budding Yeast)
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    RPS2

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUDP010

    Plasmid

    #101166

    Purpose

    E. coli/S. cerevisiae amdS shuttle vector expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 in S. pastorianus (HH-gRNASeILV6-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Insert

    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Ube2q2

    Plasmid

    #31425

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2Q2 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

27. pAG416GPD-Cerulean-YPT1

    Plasmid

    #18848

    Depositor

    Susan Lindquist

    Article

    Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    YPT1 (YPT1 Budding Yeast)
    

    Use

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

28. pXP312

    Plasmid

    #26834

    Depositor

    Nancy DaSilva, Suzanne Sandmeyer

    Article

    Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pREP4xV5_ccdB

    Plasmid

    #79016

    Purpose

    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selection

    Depositor

    Göran Akusjärvi, Pernilla Bjerling

    Type

    Empty backbone

    Use

    Tags

    double V5 epitope tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNB761

    Plasmid

    #60156

    Purpose

    Firefly luciferase (Promega) driven by ADE17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    ADE17pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCfB2991

    Plasmid

    #63643

    Purpose

    pCfB2991 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

    Depositor

    Irina Borodina, Jerome Maury

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTG005

    Plasmid

    #71386

    Purpose

    localization of yeast RNase RMP1 (RNase MRP binding protein) in yeast

    Depositor

    Mark Schmitt

    Article

    Gill et al J Cell Biol. 2006 Apr 10;173(1):35-45. E

    Insert

    RMP1 (RMP1 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    ACT1

    Available since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCB116

    Plasmid

    #53365

    Purpose

    To express Cup9p‐FLAG from the PGAL1 promoter

    Depositor

    Alexander Varshavsky

    Article

    Byrd et al EMBO J. 1998 Jan 2. 17(1):269-77.

    Insert

    Cup9 (CUP9 Budding Yeast)
    

    Use

    Tags

    Flag

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

34. pRS413 Prp5 WT

    Plasmid

    #87599

    Purpose

    Encodes Prp5 WT for expression in yeast

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

    Insert

    PRP5 (PRP5 )
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pNB789

    Plasmid

    #60166

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus-PEST
    

    Use

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    RNR1pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Ube2i

    Plasmid

    #31420

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2I Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

37. pGBT9-hSyntenin2b

    Plasmid

    #89422

    Purpose

    Two-hybrid system

    Depositor

    Fritz Rathjen

    Article

    Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

    Insert

    SDCBP2 (SDCBP2 Human)
    

    Use

    Tags

    GAL4 DBD

    Expression

    Yeast

    Mutation

    * see below

    Promoter

    ADH1

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAG423GAL-ccdB-TAP

    Plasmid

    #14269

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    TAP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJRL2 PHO5(TATA-A1T6)pr ECFP (EB#1560)

    Plasmid

    #14846

    Depositor

    Erin O'Shea

    Article

    Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

    Insert

    PHO5 promoter TATA-A1T6 (PHO5 Budding Yeast)
    

    Use

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    TATA-A1T6. TATA box mutated from TATATAAG to AATA…

    Promoter

    Available since

    May 25, 2007

    Availability

    Academic Institutions and Nonprofits only

40. pLUK

    Plasmid

    #64184

    Purpose

    harbours marker genes LEU2, URA3 and LYS2

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge