Addgene

We narrowed to 828 results for: nanobodies

Showing: 1 - 40 of 828 results

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pGEX6P1-GFP-Nanobody

Plasmid

#61838

Purpose

Expresses GST-tagged anti-GFP Nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

GFP-Nanobody

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-Nanobody

Plasmid

#70696

Purpose

Expresses GST-tagged anti-mCherry Nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

mCherry-Nanobody

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2016

Availability

Academic Institutions and Nonprofits only

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pOPINE GFP nanobody

Plasmid

#49172

Purpose

Bacterial expression of GFP nano body

Depositor

Brett Collins

Article

Kubala et al Protein Sci. 2010 Dec;19(12):2389-401. d

Insert

GFP nanobody

Use

Tags

HHHHHH

Expression

Bacterial and Mammalian

Mutation

Promoter

T7

Available since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pET29b_1_anti-GFP-nanobody

Plasmid

#210394

Purpose

Express anti-GFP nanobody in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

1_anti-GFP-nanobody

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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mTB3-BC2 nanobody

Plasmid

#210208

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

Depositor

Brian Kay

Article

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

BC2 Nanobody

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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SBFP2-anti-AlfaTag nanobody

Plasmid

#171826

Purpose

Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

Depositor

Ikuo Wada

Article

Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

Insert

SBFP2-anti-AlfaTag nanobody

Use

Tags

6xHis and SEP tag (6xD)

Expression

Bacterial

Mutation

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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cfSGFP2-anti-AlfaTag nanobody

Plasmid

#171818

Purpose

Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

Depositor

Ikuo Wada

Article

Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

Insert

cfSGFP2-anti-AlfaTag nanobody

Use

Tags

6xHis and SEP tag (6xD)

Expression

Bacterial

Mutation

Promoter

Available since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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tdTomato-anti-AlfaTag nanobody

Plasmid

#171819

Purpose

Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

Depositor

Ikuo Wada

Article

Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

Insert

tdTomato-anti-AlfaTag nanobody

Use

Tags

6xHis and SEP tag (6xD)

Expression

Bacterial

Mutation

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA5FRT/TO_HA-mAID-nanobody

Plasmid

#117713

Purpose

Expression of tetracycline-inducible HA-tagged lysine-less mAID fused to lysine-less vhhGFP4 (mAID-nanobody)

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

mAID-nanobody

Use

Tags

triple HA

Expression

Mammalian

Mutation

codon optimized mAID for murine expression

Promoter

CMV

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pHEN-CoV2-nanobody-F1

Plasmid

#194592

Purpose

Bacterial expression of F1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

F1 (S )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHEN-CoV2-nanobody-E2

Plasmid

#194590

Purpose

Bacterial expression of E2 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

E2 (S )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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nanobody(AntiGFP)-HaloTag-iLID

Plasmid

#191453

Purpose

Localizing iLID with GFP-tagged proteins

Depositor

Dorus Gadella

Article

Mahlandt et al bioRxiv 2022.11.24.517828

Insert

nanobody(AntiGFP)-HaloTag-iLID

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pHEN-CoV2-nanobody-G6

Plasmid

#194589

Purpose

Bacterial expression of G6 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

G6 (S )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHEN-CoV2-nanobody-Ty1

Plasmid

#194588

Purpose

Bacterial expression of Ty1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Nat Commun. 2020 Sep 4;11(1):4420. doi:

Insert

Ty1 (S )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHEN-CoV2-nanobody-C7

Plasmid

#194591

Purpose

Bacterial expression of C7 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

C7 (S )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET29-bdSUMO-Anti-ALFA nanobody

Plasmid

#212311

Purpose

Expression of anti-ALFA nanobody

Depositor

Arthur Laganowsky

Article

Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

Insert

anti-ALFA nanobody

Use

Tags

bdSUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P1-mCherry-Nanobody (LaM-2)

Plasmid

#162276

Purpose

Expresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

Insert

mCherry-Nanobody (LaM-2)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
HC20 pEYFPN1 anti-Homer1 nanobody

Plasmid

#135220

Purpose

Mammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabody

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Homer1 (Mouse) recombinant llama nanobody HC20 (Homer1 L. glama (llama))

Use

Tags

6xHis-HA-EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
IC65 pEGFPN1 anti-IRSp53 nanobody

Plasmid

#135227

Purpose

Mammalian expression of anti-IRSp53 (Human) recombinant llama nanobody for use as an intrabody

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-IRSp53 (Human) recombinant llama nanobody IC65 (BAIAP2 L. glama (llama))

Use

Tags

6xHis-HA-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+_HA-mAID-nanobody (Kless)

Plasmid

#117718

Purpose

mRNA synthesis of lysine-less HA-mAID-nanobdy for injection

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

mAID-nanobody

Use

Tags

3xHA

Expression

Mammalian

Mutation

codon-optimized and lysine-less mAID for murine e…

Promoter

SP6

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
GC52 pEGFPN1 anti-Gephyrin nanobody

Plasmid

#135224

Purpose

Mammalian expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an intrabody

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Gephyrin (Human) recombinant llama nanobody GC52 (GPHN L. glama (llama))

Use

Tags

6xHis-HA-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
HS69 pComb3xss anti-Homer1 nanobody

Plasmid

#134716

Purpose

Bacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Homer1 (Mouse) recombinant llama nanobody (Homer1 L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMD1 anti-GFP nanobody-Tn5

Plasmid

#215567

Purpose

Expresses anti-GFP nanobody-Tn5 fusion in bacteria with an N-terminal 3xFlag tag and a C-terminal intein-CBD-intein tag

Depositor

Isabelle Mansuy

Article

Maria A. et al bioRxiv 2024.07.12.603224

Insert

anti-GFP nanobody-Tn5 fusion

Use

Tags

3xFlag and intein-CBD

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HC25 pComb3xss anti-Homer1 nanobody

Plasmid

#134718

Purpose

Bacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Homer1 (Mouse) recombinant llama nanobody HC25 (Homer1 L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
HS63 pComb3xss anti-Homer1 nanobody

Plasmid

#134715

Purpose

Bacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Homer1 (Mouse) recombinant llama nanobody (Homer1 L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
SS80 pEGFPN1 anti-SAPAP2 nanobody

Plasmid

#135228

Purpose

Mammalian expression of anti-SAPAP (Rat) recombinant llama nanobody for use as an intrabody

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-SAPAP2 (Rat) recombinant llama nanobody SS80 (Dlgap2 L. glama (llama))

Use

Tags

6xHis-HA-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
HC87 pEYFPN1 anti-Homer1 nanobody

Plasmid

#135223

Purpose

Mammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabody

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Homer1 (Mouse) recombinant llama nanobody HC87 (Homer1 L. glama (llama))

Use

Tags

6xHis-HA-EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
HS89 pComb3xss anti-Homer1 nanobody

Plasmid

#134717

Purpose

Bacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Homer1 (Mouse) recombinant llama nanobody (Homer1 L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GC1 pComb3xss anti-Gephyrin nanobody

Plasmid

#134720

Purpose

Bacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Gephyrin (Human) recombinant llama nanobody GC1 (GPHN L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
HC89 pComb3xss anti-Homer1 nanobody

Plasmid

#134719

Purpose

Bacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Homer1 (Mouse) recombinant llama nanobody HC89 (Homer1 L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GC83 pComb3xss anti-Gephyrin nanobody

Plasmid

#134721

Purpose

Bacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Gephyrin (Human) recombinant llama nanobody GC83 (GPHN L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GC92 pComb3xss anti-Gephyrin nanobody

Plasmid

#145826

Purpose

Bacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Gephyrin (Human) recombinant llama nanobody GC92 (GPHN L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
HS22 pEYFPN1 anti-Homer1 nanobody

Plasmid

#135219

Purpose

Mammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabody

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Homer1 (Mouse) recombinant llama nanobody HS22 (Homer1 L. glama (llama))

Use

Tags

6xHis-HA-EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
HS38 pEYFPN1 anti-Homer1 nanobody

Plasmid

#135221

Purpose

Mammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabody

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Homer1 (Mouse) recombinant llama nanobody HS38 (Homer1 L. glama (llama))

Use

Tags

6xHis-HA-EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GS8 pComb3xss anti-Gephyrin nanobody

Plasmid

#145827

Purpose

Bacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Gephyrin (Human) recombinant llama nanobody GS8 (GPHN L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SS1 pComb3xss anti-SAPAP2 nanobody

Plasmid

#145818

Purpose

Bacterial expression of anti-SAPAP2 (Rat) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-SAPAP2 (Rat) recombinant llama nanobody SS1 (Dlgap2 L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SS41 pComb3xss anti-SAPAP2 nanobody

Plasmid

#145823

Purpose

Bacterial expression of anti-SAPAP2 (Rat) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-SAPAP2 (Rat) recombinant llama nanobody SS41 (Dlgap2 L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GS41 pComb3xss anti-Gephyrin nanobody

Plasmid

#145828

Purpose

Bacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Gephyrin (Human) recombinant llama nanobody GS41 (GPHN L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29_anti-MERS-Nanobody-VHH-55

Plasmid

#209417

Purpose

Express anti-MERS-Nanobody-VHH-55 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-mers-nanobody-vhh-55

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pET29_anti-MERS-Nanobody-VHH-83

Plasmid

#209418

Purpose

express anti-MERS-Nanobody-VHH-83 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-mers-nanobody-vhh-83

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

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