Purpose
Expresses the reverse tetracycline activator (rtTA) and T7 polymerase; Plasmid #1 of the tet-on overexpression system
Inserts
T7 polymerase with NLS and P278L
reverse tetracycline transactivator (rtTA)
Expression
Yeast
Mutation
Contains M2-SE-G72P mutations, described in Addge…
Promoter
TDH3 and tetracycline-responsive element (TRE) an…
Available since
June 24, 2025
Availability
Academic Institutions and Nonprofits only