Addgene

We narrowed to 3,192 results for: zebrafish

Showing: 181 - 200 of 3192 results

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acta1-Cavin4b-Clover

Plasmid

#163854

Purpose

Zebrafish muscle specific expression of Cavin4b-Clover. Parton lab clone DQZ

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

Cavin4b (cavin4b Zebrafish)

Use

Zebrafish expression

Tags

Clover

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9 ABE-Umax-ex1

Plasmid

#222141

Purpose

Adenine base editor with modified TadA variant for extended window for A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Expression

Mutation

Promoter

T3

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcs2+spCas9 ABE-Umax-nanos

Plasmid

#222142

Purpose

Adenine base editor with modified TadA variant for germline A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9 with nanos UTR

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Expression

Mutation

Promoter

SP6

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9 ABE-Umax-ex2-rest1

Plasmid

#222143

Purpose

Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Expression

Mutation

Promoter

T3

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9 ABE-Umax-ex1-rest1

Plasmid

#222140

Purpose

Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Expression

Mutation

Promoter

T3

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pT2h70CRET(#325)

Plasmid

#184060

Purpose

cyclofen-inducible CRE activation in zebrafish transient transgenic

Depositor

Sophie Vriz

Article

Feng et al Sci Rep. 2017 Aug 23;7(1):9195. doi: 10.

Insert

CRE-ERT2

Use

Zebrafish transient transgenesis

Tags

Expression

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Hsp70-4

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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tol2-mpx-Dendra2

Plasmid

#29574

Depositor

Anna Huttenlocher

Article

Yoo et al J Leukoc Biol. 2011 Jan 19. ():.

Insert

mpx-Dendra 2

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2011

Availability

Academic Institutions and Nonprofits only
pDONR221-cxadr

Plasmid

#192734

Purpose

Gateway entry vector encoding zebrafish cxadr

Depositor

Summer Thyme

Insert

cxadr (cxadr Zebrafish)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

None

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-nrip1a

Plasmid

#192731

Purpose

Gateway entry vector encoding zebrafish nrip1a

Depositor

Summer Thyme

Insert

nrip1a (LOC796407 Zebrafish)

Use

Gateway entry vector

Tags

Expression

Mutation

E662Q; S895P; H967Q

Promoter

None

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-btg3

Plasmid

#192735

Purpose

Gateway entry vector encoding zebrafish btg3

Depositor

Summer Thyme

Insert

btg3 (btg3 Zebrafish)

Use

Gateway entry vector

Tags

Expression

Mutation

H211Y

Promoter

None

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-erg

Plasmid

#192728

Purpose

Gateway entry vector encoding zebrafish erg

Depositor

Summer Thyme

Insert

erg (erg Zebrafish)

Use

Gateway entry vector

Tags

Expression

Mutation

G199D

Promoter

None

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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MiniCoopR mitfa:BRAF

Plasmid

#118846

Purpose

Expresses human BRAF V600E mutant and zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

BRAF V600E (BRAF Human, Zebrafish)

Use

Zebrafish expression, tol2

Tags

Expression

Mutation

BRAF V600E

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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MiniCoopR mitfa:KIT K642E

Plasmid

#118849

Purpose

Expresses human KIT K642E mutant and zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

KIT K642E (KIT Human, Zebrafish)

Use

Zebrafish expression, tol5

Tags

Expression

Mutation

KIT K642E

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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MiniCoopR mitfa:NRAS

Plasmid

#118847

Purpose

Expresses human NRAS Q61R mutant and zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

NRAS Q61R (NRAS Human, Zebrafish)

Use

Zebrafish expression, tol3

Tags

Expression

Mutation

NRAS Q61R

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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MiniCoopR mitfa:KIT L576P

Plasmid

#118848

Purpose

Expresses human KIT L576P mutant and zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

KIT L576P (KIT Human, Zebrafish)

Use

Zebrafish expression, tol4

Tags

Expression

Mutation

KIT L576P

Promoter

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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gfap-EGFP donor

Plasmid

#65564

Purpose

Intron targeting-mediated EGFP knockin at the zebrafish gfap locus

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

gfap-EGFP (gfap Zebrafish)

Use

Zebrafish injection

Tags

Expression

Mutation

Promoter

none

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pT22gCfpUbiG4FER4P2A(#249)

Plasmid

#184070

Purpose

cyclofen-inducible GAL4 activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Feng et al Sci Rep. 2017 Aug 23;7(1):9195. doi: 10.

Insert

Gal4bdFF-ERT2-P2A

Use

Zebrafish transgenesis (blue eyes)

Tags

Expression

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSGNluc

Plasmid

#186705

Purpose

A high-sensitivity bi-directional reporter to monitor NF-κB activity in cell culture and zebrafish in real time

Depositor

Maria Leptin, Baubak Bajoghli

Article

Kuri et al J Cell Sci. 2017 Feb 1;130(3):648-657. d

Inserts

GFP
luciferase

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-mito-Dendra2

Plasmid

#107590

Purpose

Expresses mitochondria localized Dendra2 in zebrafish neutrophils

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

mito-Dendra2

Use

Tags

mitochondira localization sequence

Expression

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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MLM3613

Plasmid

#42251

Purpose

Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

Streptococcus pyogenes Cas9

Use

CRISPR; Zebrafish expression

Tags

Expression

Mutation

Promoter

CMV

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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