2,159 results

1. pUFlip-floxed2A-mRFP-; gcry1:BFP -1

   Plasmid

   #173887

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-mRFP; gcry1: BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. flk1 promoter

   Plasmid

   #26436

   Depositor

   Didier Stainier

   Article

   Jin et al Development. 2005 Dec . 132(23):5199-209

   Insert

   flk1 promoter
   

   Use

   Topo cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-PLIN2-tdTOMATO

   Plasmid

   #172533

   Purpose

   PLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloning

   Depositor

   Richard White

   Article

   Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

   Insert

   plin2-tdtomato
   

   Use

   Tags

   Expression

   Mutation

   S80R, A81S, E144D, I148F, and R415H mutations in …

   Promoter

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTol2CG2-ubi:QFGal4-SV40pA

   Plasmid

   #155119

   Purpose

   Tol2 vector for ubiquitous expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   ubi:QFGal4 (ubb Zebrafish, Neurospora crassa, Budding Yeast)
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   ubiquitin B

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. 14UAS PSD95:GFP 5UAS DSRedExpress

   Plasmid

   #74315

   Purpose

   Encodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafish

   Depositor

   Martin Meyer, Stephen Smith

   Article

   Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub

   Inserts

   zebrafish PSD95
   DsRedExpress
   

   Use

   Tags

   EGFP

   Expression

   Mutation

   Y599H and I793S (please see depositor comment bel…

   Promoter

   Available since

   May 19, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pKHR7

   Plasmid

   #74624

   Purpose

   a cmlc2::mCherry (red heart) reporter gene was inserted at the 3’end of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.

   Depositor

   David Grunwald

   Article

   Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

   Insert

   cmlc2::mCherry
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   cmlc2

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPRISM-gal4-VP16-cmlc2-mRFP

   Plasmid

   #117781

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   gal4/VP16
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Hox D4a

   Plasmid

   #36469

   Depositor

   Robert Ho

   Article

   Prince et al Development. 1998 Feb . 125(3):407-20.

   Insert

   Hox D4a (hoxd4a Zebrafish)
   

   Use

   Riboprobe synthesis

   Tags

   Expression

   Bacterial

   Mutation

   Q100H, A101T, G125C and R189W

   Promoter

   Available since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS

   Plasmid

   #78251

   Purpose

   Can be used to subclone in tagged gene of interest into inducible motor neuron expression vector.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Inserts

   -3mnx1 (mnx1 Zebrafish)
   -loxP-mTagBFP2-stop-
   mCherry-NS-MCS
   

   Use

   Zebrafish motor neuron inducible expression

   Tags

   Expression

   Mutation

   Promoter

   -3mnx1

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1

    Plasmid

    #198240

    Purpose

    For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sites

    Depositor

    Didier Stainier

    Article

    Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.

    Inserts

    Zebrafish fabp10a promoter:floxedBFP, Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
    Zebrafish cryaa promoter:mcherry fluorescent protein coding sequence (cryaa Zebrafish)
    

    Use

    Zebrafish transgenesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCK074 p5E exorh:EGFP

    Plasmid

    #195951

    Purpose

    Gateway p5E vector with reporter

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    exorh:EGFP
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. p5E-mnx1

    Plasmid

    #82402

    Purpose

    Gateway-compatible zebrafish mnx1 promoter

    Depositor

    Judith Eisen

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    mnx1 promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTol2_claudink:Cas9green; erbb2_gRNA171

    Plasmid

    #199339

    Purpose

    pDEL142; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous erbb2 sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    claudink
    Cas9
    erbb2 sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. zCREST3:Cas9green; EGF_gRNA

    Plasmid

    #199333

    Purpose

    pDEL158; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 EGF sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    zCREST3
    Cas9
    nrg1 EGF sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTol2_NBT:Cas9green; erbb2_gRNA171

    Plasmid

    #199337

    Purpose

    pDEL134; transgenic construct to express cell-specific Cas9 in neurons; ubiquitous erbb2 sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    NBT
    Cas9
    erbb2 sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTol2_claudink:Cas9green; control_gRNA

    Plasmid

    #199336

    Purpose

    pDEL143; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous control sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    claudink
    Cas9
    control sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. p5'Entry-lck-5.5kb

    Plasmid

    #58890

    Purpose

    provides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenes

    Depositor

    Jeffrey Yoder

    Insert

    zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
    

    Use

    Gateway 5' entry clone

    Tags

    Expression

    Mutation

    mutated single nucleotide (T-->C, 13th nucleot…

    Promoter

    lymphocyte-specific protein tyrosine kinase (lck)…

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST mfap4:tdTomato

    Plasmid

    #135214

    Purpose

    Red tagged macrophages

    Depositor

    David Tobin

    Article

    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

    Insert

    mfap4 promoter and tdTomato
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2+ gfp-zASC

    Plasmid

    #98703

    Purpose

    GFP tagged zebrafish ASC in the pCS2+ vector for in vitro transcription

    Depositor

    Anna Huttenlocher

    Article

    Vincent et al Cell Microbiol. 2016 Apr;18(4):591-604.

    Insert

    Apoptosis associated speck like protein containing CARD (pycard Zebrafish)
    

    Use

    Zebrafish/xenopus expression

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTol1:uas-myc-act-map2k2b-2a-tdTomato

    Plasmid

    #104721

    Purpose

    UAS-driven expression of activated MEK

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    activated map2k2b (map2k2b Zebrafish)
    

    Use

    Zebrafish transposon

    Tags

    2A-tdTomato and myc tag

    Expression

    Mutation

    serines 221 and 225 to aspartic acid

    Promoter

    10xUAS

    Available since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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