2,189 results
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Plasmid#78250PurposeCan be used to subclone in gene of interest into blue motor neuron expression vector.DepositorInsertsUseZebrafish motor neuron expressionPromoter-3mnx1Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only
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Hox A11a
Plasmid#36462DepositorAvailable SinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCS2TAL3-RR-zrif1_exon8
Plasmid#194434PurposeRight (RR) TALEN for mutating zebrafish rif1 geneDepositorAvailable SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2TAL3-DD-zrif1_exon8
Plasmid#194435PurposeLeft (DD) TALEN for mutating zebrafish rif1 geneDepositorAvailable SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
mKate2-DrCavin1b in pT3TS-Dest
Plasmid#194295PurposeIn vitro transcription of mKate2 tagged zebrafish cavin1b from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LACDepositorInsertcavin1b (cavin1b Zebrafish)
UseIn vitro transcription of mrnaAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2KXIG (Tol2)-tp63:ikk1:GFP
Plasmid#105646PurposeIkk1(alpha) expression in basal keratinocytesDepositorAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1
Plasmid#198240PurposeFor I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sitesDepositorInsertsUseZebrafish transgenesisAvailable SinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST_6xUAS:mNeonGreen-Rab5a
Plasmid#192355PurposezfRabaa tagged with mNeonGreen, expressed under the UAS promoterDepositorAvailable SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
p160Tol2-her4.3:switchCas9
Plasmid#227680PurposeExpression of cre inducible and her4.3 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs for SABER-seq in zebrafishDepositorInsertsloxP-dsRed-loxP-Cas9-t2A-GFP
4xU6:sgRNA
UseCRISPRPromoterU6 and her4.3Available SinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
p5E-mnx1
Plasmid#82402PurposeGateway-compatible zebrafish mnx1 promoterDepositorInsertmnx1 promoter
Available SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP
Plasmid#158963PurposeCre-dependet Cas9-GFP expressionDepositorInsertshsp70l promoter
floxed Non-FP cassette
Cas9-GFP
UseCRISPR and Cre/LoxAvailable SinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
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SI10-Brevican
Plasmid#46300DepositorAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestRFP_RNAseH1
Plasmid#65785Purposeexpresses RFP-tagged RNAseH1 in mammalian cellsDepositorAvailable SinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-mRFP-; HE1A:BFP -1
Plasmid#180008PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-mRFP; HE1A: BFP
UseSynthetic BiologyAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus
Plasmid#105127PurposeUsed along with I-SceI enzyme to generate transgenic zebrafish expressing hepatocyte-specific Xenopus activated beta-catenin and lens-specific Venus fluorescent proteinDepositorInsertsUseZebrafish transgenesisPromotercryaa (crystallin) and fabp10aAvailable SinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ centrin-mKate2
Plasmid#105941Purposein vitro transcription, zf centrin N-terminally tagged with mkate2DepositorInsertCentrin
TagsmKate2ExpressionBacterialAvailable SinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT-sox10-cytoBirA-2A-mCherry_Ras
Plasmid#80062PurposeSox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterSox10 promoterAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-TagRFP-gcry-TagBFP
Plasmid#117765PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTol1:uas-myc-act-map2k2b-2a-tdTomato
Plasmid#104721PurposeUAS-driven expression of activated MEKDepositorInsertactivated map2k2b (map2k2b Zebrafish)
UseZebrafish transposonTags2A-tdTomato and myc tagMutationserines 221 and 225 to aspartic acidPromoter10xUASAvailable SinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only