2,162 results

1. pCK029 exorh:EGFP

   Plasmid

   #195980

   Purpose

   Expression construct

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   exorh:EGFP
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTone-gata2aECE-Kaede

   Plasmid

   #132976

   Purpose

   gata2a endothelial enhancer (x6) and basal promoter driving kaede in pTol1 backbone

   Depositor

   Nathan Lawson

   Article

   Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

   Inserts

   Kaede
   gata2a endothelial enhancer (x6) and a carp b-actin basal promoter
   

   Use

   Unspecified; Transposon-mediated

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6a:sgRNA(tyr)

   Plasmid

   #64250

   Purpose

   expresses sgRNA(tyr) under U6a promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Insert

   U6a:sgRNA (tyr)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2+ Zf GFP-vclb

   Plasmid

   #185411

   Purpose

   GFP tagged form of zebrafish vinculin b. For in vitro transcription (SP6) or expression through CMV

   Depositor

   Nicolas David

   Article

   Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

   Insert

   vinculin b (vclb Zebrafish)
   

   Use

   In vitro synthesis of mrna

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. p5E_(-3kb)nrg1 type II

   Plasmid

   #199393

   Purpose

   pDEL61; Gateway 5' Entry clone encoding nrg1 type II upstream regulatory sequence

   Depositor

   William Talbot

   Article

   Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

   Insert

   (-3kb)nrg1 type II
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. p5E_ngn1_LSE_C

   Plasmid

   #199395

   Purpose

   pDEL98; Gateway 5' Entry clone encoding select ngn1 upstream regulatory sequence

   Depositor

   William Talbot

   Article

   Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

   Insert

   LSE_C
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPRISM-gal4-VP16-gcry-TagBFP

   Plasmid

   #117777

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   gal4/VP16
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMT-sox10-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80063

   Purpose

   Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Sox10 promoter

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP1-sox17 promoter

   Plasmid

   #31400

   Depositor

   Didier Stainier

   Insert

   sox17 promoter (sox17 Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pUFlip-floxed2A-KalTA4-; HE1A:BFP -0

    Plasmid

    #180010

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-KalTA4; HE1A: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3E-rab7a

    Plasmid

    #109723

    Purpose

    Multisite gateway vector for 3' tagging with rab7a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab7a (rab7a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2+ Zf GFP-vcla

    Plasmid

    #185410

    Purpose

    GFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMV

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    vinculin a (vcla Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. p3E-U6a-U6c-ndufs2 guide

    Plasmid

    #107601

    Purpose

    An entry vector with U6a and U6c promoter driving ndufs2 guide RNAs expression

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    ndufs2 guide RNA sequences
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGTag-NLS-TagRFP-SV40

    Plasmid

    #117805

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    TagRFP
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Gfp-zf-vasa

    Plasmid

    #167323

    Purpose

    Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.

    Depositor

    Goro Yoshizaki

    Article

    Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

    Insert

    zf vasa 3'UTRs (ddx4 Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. zCD2

    Plasmid

    #122031

    Purpose

    Expresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.

    Depositor

    David Christianson

    Article

    Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

    Insert

    Zebrafish HDAC6 Catalytic Domain 2
    

    Use

    Tags

    His6-MBP tag with cleavable TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST mfap4:icre-p2a-tomato

    Plasmid

    #135217

    Purpose

    Cre coexpressed with tdTomato in macrophages

    Depositor

    David Tobin

    Article

    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

    Insert

    mfap4 promoter with iCre and Tomato
    

    Use

    Cre/Lox; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. p5E-lyzC

    Plasmid

    #107591

    Purpose

    An entry vector with a lyzC promoter

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    Lysozyme C promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTol2_LSEC:Cas9green; erbb2_gRNA171

    Plasmid

    #199338

    Purpose

    pDEL135; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous erbb2 sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    LSEC
    Cas9
    erbb2 sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUFlip-floxed2A-KalTA4-; HE1A:BFP -1

    Plasmid

    #180011

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-KalTA4; HE1A: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge