2,163 results

1. pDEST mfap4:icre-p2a-tomato

   Plasmid

   #135217

   Purpose

   Cre coexpressed with tdTomato in macrophages

   Depositor

   David Tobin

   Article

   Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

   Insert

   mfap4 promoter with iCre and Tomato
   

   Use

   Cre/Lox; Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT-sox10-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80063

   Purpose

   Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Sox10 promoter

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Zebrafish ArcLight Q175

   Plasmid

   #53616

   Purpose

   Genetically encoded voltage sensor ArcLight with improved kinetics

   Depositor

   Vincent Pieribone

   Article

   Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

   Insert

   Zebrafish ArcLight-Q175 (tpte Zebrafish, Synthetic, Aequorea victoria)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Dr-VSD contains R153Q mutation and an amino acid …

   Promoter

   CMV

   Available since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTol2_LSEC:Cas9green; erbb2_gRNA171

   Plasmid

   #199338

   Purpose

   pDEL135; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous erbb2 sgRNA

   Depositor

   William Talbot

   Article

   Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

   Inserts

   LSEC
   Cas9
   erbb2 sgRNA
   

   Use

   Tol2 destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP1-sox17 promoter

   Plasmid

   #31400

   Depositor

   Didier Stainier

   Insert

   sox17 promoter (sox17 Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pGTag-NLS-TagRFP-SV40

   Plasmid

   #117805

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   TagRFP
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. zCD2

   Plasmid

   #122031

   Purpose

   Expresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.

   Depositor

   David Christianson

   Article

   Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

   Insert

   Zebrafish HDAC6 Catalytic Domain 2
   

   Use

   Tags

   His6-MBP tag with cleavable TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. p5E-lyzC

   Plasmid

   #107591

   Purpose

   An entry vector with a lyzC promoter

   Depositor

   Qing Deng

   Article

   Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

   Insert

   Lysozyme C promoter
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKE13-ISceI-fabp10a:CreERT2

   Plasmid

   #198242

   Purpose

   For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter driving tamoxifen-inducible Cre recombinase

   Depositor

   Didier Stainier

   Article

   Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.

   Inserts

   Zebrafish fabp10a promoter:Cre recombinase (Cre) fused to a mutant estrogen ligand-binding domain (ERT2), polyA (fabp10a Zebrafish)
   Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)
   

   Use

   Zebrafish transgenesis

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Control-cx43.4-48H-pGTag

    Plasmid

    #117818

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    TagRFP
    

    Use

    Synthetic Biology

    Tags

    CAAX

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3E_he1a:ECFP

    Plasmid

    #113880

    Purpose

    p3E entry vector for Three Multisite Gateway

    Depositor

    Marnie Halpern

    Insert

    he1a (he1.1 Zebrafish)
    

    Use

    Zebrafish danio expression

    Tags

    ECFP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. MiniCoopR mitfa:NRAS

    Plasmid

    #118847

    Purpose

    Expresses human NRAS Q61R mutant and zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    NRAS Q61R (NRAS Zebrafish, Human)
    

    Use

    Zebrafish expression, tol3

    Tags

    Expression

    Mutation

    NRAS Q61R

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPRISM-gal4-VP16-gcry-eGFP

    Plasmid

    #117779

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    gal4/VP16
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. p5E-1.7kbfabp6

    Plasmid

    #159087

    Purpose

    p5E with promoter sequence 1.7kb upstream of zebrafish fabp6 gene

    Depositor

    John Rawls

    Article

    Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

    Insert

    1.7kb fabp6 promoter (fabp6 Zebrafish)
    

    Use

    Promoter fragment 5' entry vector for tol2 c…

    Tags

    Expression

    Mutation

    Promoter

    1.7kb fabp6

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. flk1 promoter

    Plasmid

    #26436

    Depositor

    Didier Stainier

    Article

    Jin et al Development. 2005 Dec . 132(23):5199-209

    Insert

    flk1 promoter
    

    Use

    Topo cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCK052 p5E exorh:mCherry

    Plasmid

    #195953

    Purpose

    Gateway p5E vector with reporter

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    exorh:mCherry
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKHR7

    Plasmid

    #74624

    Purpose

    a cmlc2::mCherry (red heart) reporter gene was inserted at the 3’end of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.

    Depositor

    David Grunwald

    Article

    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

    Insert

    cmlc2::mCherry
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    cmlc2

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Tol2-krt4-GFP

    Plasmid

    #97151

    Purpose

    label apical epithelial cells in zebrafish

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Insert

    AcGFP
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDestTol2CG2--3.5ubb:plin2-tdtomato

    Plasmid

    #172534

    Purpose

    Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis marker

    Depositor

    Richard White

    Article

    Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

    Insert

    -3.5ubb:plin2-tdtomato
    

    Use

    Tags

    Expression

    Mutation

    S80R, A81S, E144D, I148F and R415H mutations obse…

    Promoter

    zebrafish ubb

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTol2CG2-ubi:QFGal4-SV40pA

    Plasmid

    #155119

    Purpose

    Tol2 vector for ubiquitous expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    ubi:QFGal4 (ubb Zebrafish, Neurospora crassa, Budding Yeast)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    ubiquitin B

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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