2,223 results

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pCS2+Paxillin-mKate

Plasmid

#105974

Purpose

in vitro transcription, paxillin coding sequence without stop codon-paxilin is a protein of focal adhesions

Depositor

Caren Norden

Article

Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

Insert

Paxillin

Tags

mKate

Expression

Bacterial

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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TALEN1297

Plasmid

#32279

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting hey2 (hey2 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pCSegfpN5ICD

Plasmid

#22467

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

Notch3 (notch3 Zebrafish)

Use

Injection construct

Available Since

March 8, 2010

Availability

Academic Institutions and Nonprofits only
ZP60 Axin2

Plasmid

#16882

Depositor

Randall Moon

Article

Weidinger et al Curr Biol. 2005 Mar 29. 15(6):489-500.

Insert

axin2 (axin2 Zebrafish)

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
ZE15 pCS2P+ wnt8 ORF2

Plasmid

#17049

Depositor

Randall Moon

Insert

wnt8 (wnt8a Zebrafish)

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pCS2 you

Plasmid

#11084

Depositor

William Talbot

Article

Woods et al PLoS Biol. 2005 Mar . 3(3):e66.

Insert

you (scube2 Zebrafish)

Use

Zebrafish

Available Since

Dec. 8, 2005

Availability

Academic Institutions and Nonprofits only
fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0; cmlc2:EGFP

Plasmid

#230075

Purpose

Partial ablation using NTR2 and subsequent lineage tracing of regenerating hepatocytes in zebrafish.

Depositor

Sumeet Pal Singh

Article

Hovhannisyan et al NPJ Regen Med. 2026 Mar 30. doi: 10.1038

Insert

lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0

Use

Cre/Lox; Zebrafish expression

Promoter

fabp10a

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-Opto-Acvr1ba-P2A-Acvr2ba

Plasmid

#232640

Purpose

Optogenetic Nodal activators from Sako et al. 2016 (Cell Reports) separated by a P2A peptide for singe-transcript expression

Depositor

Katherine Rogers

Article

Iannucci et al bioRxiv [Preprint]. 2025 Apr 19:2025.04.

Insert

Acvr1ba + VfLOV domain and Acvr2ba + VfLOV domain

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST_6xUAS:mNeonGreen-Rab5a

Plasmid

#192355

Purpose

zfRabaa tagged with mNeonGreen, expressed under the UAS promoter

Depositor

Francesca Peri

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

Rab5a (rab5aa Zebrafish)

Tags

mNeonGreen

Expression

Mammalian

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; HE1A:BFP -2

Plasmid

#180009

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; HE1A: BFP

Use

Synthetic Biology

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiCD19_eZ3_Gal4VP64

Plasmid

#169917

Purpose

Expression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.

Depositor

Zhili Rong

Article

Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

Insert

antiCD19-Notch3-GL4VP64

Use

Lentiviral

Expression

Mammalian

Mutation

Additional EGF repeat between the extracellular d…

Promoter

PGK

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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Gfp-zf-vasa

Plasmid

#167323

Purpose

Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.

Depositor

Goro Yoshizaki

Article

Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

Insert

zf vasa 3'UTRs (ddx4 Zebrafish)

Expression

Bacterial

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pME-lamp1

Plasmid

#109559

Purpose

Multisite gateway vector for 5' tagging with lamp1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

lamp1 (lamp1b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pPRISM-TagRFP-gcry-eGFP

Plasmid

#117767

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pGTag-eGFP-CAAX-B-actin

Plasmid

#117816

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Tags

CAAX

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Tol2-Pax3a_enh5-E1b:eGFP-Tol2

Plasmid

#119061

Purpose

Fluorescent vector with zebrafish pax3a enhancer 5; Tol2 version

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

pax3a_enh5-E1b:eGFP

Expression

Bacterial

Promoter

E1b minimal promoter

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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MiniCoopR U6:gRNA cdkn2a, mitfa:Cas9

Plasmid

#118842

Purpose

Targets cdkn2a and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

cdkn2a gRNA (LOC100329528 Zebrafish)

Use

CRISPR; Tol2

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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crestin

Plasmid

#89394

Purpose

For in situ hybridization, linearize with Sac I use T7 polymerase

Depositor

Marnie Halpern

Article

Rubinstein et al Genesis. 2000 Jan;26(1):86-97.

Insert

crestin (crestin Zebrafish)

Use

In situ probe

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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p5E-dusp6

Plasmid

#82584

Purpose

5' entry vector containing FGF-responsive regulatory element of the zebrafish dusp6 promoter for FGF-responding cells

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

FGF-responsive element of the dusp6 promoter

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pHis-zfCSF3a

Plasmid

#65612

Purpose

express soluble His-tagged zebrafish CSF3a/Gcsfa in E. coli

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2011 Aug 4;118(5):1274-82. doi: 1

Insert

Csf3a (csf3a Zebrafish)

Tags

(His)6

Expression

Bacterial

Promoter

T7

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only