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Plasmid#15444DepositorInsertTissue factor promoter (F3 Human)
UseLuciferaseTagsExpressionMammalianMutationBoth AP-1 sites mutatedPromoterAvailable sinceJuly 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_FL MUT TET1
Plasmid#124081PurposeExpression of catalytic inactive form of full-length TET1DepositorInsertTET1 - full length, mutated (TET1 Human)
UseTagsFlag and HAExpressionMammalianMutationH1672Y, D1674APromoterEF1 alphaAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ADARcd-YTHmut
Plasmid#194431PurposeADAR1 catalytic domain E488Q fused to YTHmutDepositorInsertADARcd fused to YTHmut domain (ADAR Human)
UseTagsADARcd-YTHmut-HAExpressionMammalianMutationm6A binding region of YTH domain is deletedPromoterAvailable sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSPL3-hRPH3A_c.1853Gmut
Plasmid#190479Purposeminigene assayDepositorInsertRPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
UseTagsExpressionMammalianMutationc.1853A>GPromoterSV40Available sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_CD MUT TET1
Plasmid#124083PurposeExpression of mutated catalytic domain only form of TET1DepositorInsertTET1 - catalytic domain mutated (TET1 Human)
UseTagsFlag and HAExpressionMammalianMutationH1672Y, D1674APromoterEF1 alphaAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Pro Mut E2
Plasmid#48750PurposeExpression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)DepositorInsertmPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
UseLuciferaseTagsExpressionMutationMutated E-box2 sequence from CACGTT to GCTAGTPromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmIL-6 mut C/EBP
Plasmid#61291Purposedrives luciferase from mouse IL-6 promoter with mutant C/EBP (NF-IL-6) siteDepositorInsertIL-6 promoter (Il6 Mouse)
UseLuciferaseTagsExpressionMammalianMutationMutated C/EBP binding site from ACATTGTGCAATCT to…PromoterIL-6 promoter with mutant C/EBP binding siteAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_UBM-mut
Plasmid#187150Purposemammalian cell expression of FLAG-HUWE1 L2976A/P2977ADepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationL2976A/P2977APromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_UIM-mut
Plasmid#187148Purposemammalian cell expression of FLAG-HUWE1 A1376G/S1382ADepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationA1376G/S1382APromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_UBA-mut
Plasmid#187146Purposemammalian cell expression of FLAG-HUWE1 M1328A/F1330ADepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationM1328A/F1330APromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS5-Mt-mut
Plasmid#120830PurposeEctopic expression of ZIKV proteinDepositorInsertZIKV NS5 E218A "Mtase mut"
UseTagsV5ExpressionMammalianMutationE218APromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
PGL4-4X Mut Renilla
Plasmid#70040PurposeControl plasmid for testing 5'UTR folding elementsDepositorInsertRandom sequence matched for GC-content
UseLuciferaseTagsrenilla luciferaseExpressionMammalianMutationPromoterSV40Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
RSRF-Luc-2mut
Plasmid#31819DepositorInsertMutant MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
UseLuciferaseTagsExpressionMammalianMutationtwo CTATATTTAG RSRF sites mutated to CGATATTTCGPromotermutated MEF2-dependent (-307 to -242 of Nur77/NR4…Available sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMSCV LDTS-LIRmut
Plasmid#189001Purposelipophagy-inducing adaptor protein, negative controlDepositorInsertLDTS-LIRmut
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 _3xHA-Ago2_RNAmut
Plasmid#73539PurposeInducible lentiviral expression of Ago2_RNAmutDepositorInsertAgo2 (Ago2 Mouse)
UseLentiviralTags3xHAExpressionMammalianMutationSmall RNA binding mutant PAZ domain Y311A and F31…PromoterTRE promoter, Tet ONAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
FH-TET3-HxDmut-pEF
Plasmid#127896PurposeExpresses ten-eleven-translocation 3 (TET3) containing HxD mutation at H1077Y and D1079ADepositorInsertTET3-HxDmut (TET3 Human)
UseTagsHA and FlagExpressionMammalianMutationPromoterEF-1aAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNS19-LV-mutRFP-2A-GFP
Plasmid#113716PurposeLentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)DepositorInsertmutRFP-2A-GFP-IRES-Puro
UseLentiviral; Reporter systemTagsExpressionMammalianMutationY64L (mutRFP)PromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-pCaf-mCherry
Plasmid#192860PurposepMUT2 based vector for sensing caffeine in E. coli Nissle 1917DepositorInsertNanobody-CadC fusion protein and cognate promoter inducible by caffeine
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT2 NCmut-msfGFP
Plasmid#180320Purposemammalian expression of human SEPT2 NCmut fused to monomeric superfolder GFPDepositorInsertSEPTIN2 (SEPTIN2 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT2 F20D, V27DPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 _3xHA-Ago2_CATmut
Plasmid#73540PurposeInducible lentiviral expression of Ago2_CATmutDepositorInsertAgo2 (Ago2 Mouse)
UseLentiviralTags3xHAExpressionMammalianMutationCatalytic mutant PIWI domain D670APromoterTRE promoter, Tet ONAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
TBRE-TK-Luc-MUT
Plasmid#36245DepositorInsertTwo copies of the Brachyury T-box site
UseTagsLuciferaseExpressionMammalianMutationMutations in the Brachyury palindromic element:CA…PromoterTK minimal promoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL2-Mcl-1 mut
Plasmid#19133DepositorInsertMcl-1 promoter (MCL1 Human)
UseLuciferaseTagsExpressionMammalianMutationNFkB binding-site mutant, the sequence GGGGTCTTCC…PromoterAvailable sinceOct. 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
LUC45 (L/Sec/mutP)
Plasmid#112148PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with Cysteine 258 mutated to selenocysteine (U) and MUTANT AUGA PHGPx SECIS core (Gpx4 Rat, Photinus pyralis)
UseTagsExpressionMammalianMutationCysteine 258 mutated to selenocysteine (U) and SE…PromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-pSMANTIS_1kb_KLF2mut 899
Plasmid#194193PurposeIncludes the promoter (1kb) of SMTS with mutated KLF binding sites (position 899)DepositorInsertSMANTIS promoter (1kb) (SMANTIS Human)
UseLuciferaseTagsExpressionMutationmutated KLF binding sites (position 899)PromoterSMANTIS promoter (1kb), mutatedAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
FH-TET3-CXXCmut-pEF
Plasmid#127895PurposeExpresses ten-eleven-translocation 3 (TET3) containing CXXC mutation 80THQ82 to AAADepositorInsertTET3-CxxCmut (TET3 Human)
UseTagsHA and FlagExpressionMammalianMutationPromoterEF-1aAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NPYmut
Plasmid#208679PurposeExpresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in neuronsDepositorInsertGPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
EE-mut SV40 luc
Plasmid#8460DepositorInserthuman cyclin E promoter fragment, mutant (CCNE1 Human)
UseLuciferaseTagsExpressionMammalianMutationE2F sites I, II, and III mutated.PromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
CMV-CDX2-MUT
Plasmid#16554DepositorInsertCDX2 (CDX2 Human)
UseAdenoviralTagsExpressionMammalianMutationRKO mutationPromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV_mApple-SEPT7 Gmut2
Plasmid#180317Purposemammalian expression of human SEPT7 Gmut2 fused to mAppleDepositorInsertSEPTIN7 (SEPTIN7 Human)
UseTagsmAppleExpressionMammalianMutationSEPT7 H279DPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
-227 hTF (mutant NF-kB)
Plasmid#15443DepositorInsertTissue factor promoter (F3 Human)
UseLuciferaseTagsExpressionMammalianMutationNF-kappaB binding domain mutatedPromoterAvailable sinceJuly 27, 2007AvailabilityAcademic Institutions and Nonprofits only