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Showing: 451 - 500 of 2330 results

  6. pCDH-EF1-Luc2-P2A-tdTomato

    Plasmid
    #72486
    Purpose
    Express Luc2 and tdTomato under EF1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    Luc2-P2A-tdTomato
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Jan. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72486-plasmid-map-sequence-id-418875

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  7. mEmerald EFHC2-N-18

    Plasmid
    #54077
    Purpose
    Localization: EF-Hand Domain , Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    EFHC2 (EFHC2 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. pAAV-EF1α-DIO-GtACR2-P2A-Voltron2ST-W3SL

    Plasmid
    #217676
    Purpose
    AAV-mediated, Cre-dependent co-expression of GtACR2 and soma-targeted Voltron2 (ORCHID) for assessing inhibitory receptor driving force through voltage imaging with concurrent activation of GtACR2.
    Depositor
    Joseph Raimondo
    Article
    Selfe et al bioRxiv 2023.08.30.555464
    Insert
    GtACR2-P2A-Voltron2ST
    Use
    AAV
    Tags
    Soma targeting sequence (Kv2.1) on Voltron2 gene …
    Expression
    Mammalian
    Mutation
    Promoter
    EF‐1α
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217676-plasmid-map-sequence-id-432468

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  11. pPKm-300

    Plasmid
    #104626
    Purpose
    Expresses pSIN - EF-1alpha - MTS - tFd, vector encoding mitochondrial-tagged Thermosynechococcus elongates Ferredoxin (Fd), under EF-1alpha promoter
    Depositor
    Todd Coleman
    Article
    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717
    Insert
    tFd
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1alpha
    Available since
    Availability
    Academic Institutions and Nonprofits only
    104626-plasmid-map-sequence-id-204688

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  12. pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR

    Plasmid
    #207805
    Purpose
    E-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.
    Depositor
    Koen Breyne
    Article
    Maalouf et al Mol Ther. 2023 Jul 5;31(7):2206-2219. do
    Insert
    E-NoMi
    Use
    Lentiviral
    Tags
    3xFlag tag, copGFP, mCherry, and nanoluciferase
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1alpha
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    207805-plasmid-map-sequence-id-419158

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  20. pMycoFos

    Plasmid
    #84577
    Purpose
    Shuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium. Copy number is controllable by arabinose in E.coli.
    Depositor
    Nicholas Coleman
    Article
    Ly et al J Microbiol Methods. 2011 Sep;86(3):320-
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 7, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    84577-plasmid-map-sequence-id-175425

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  22. pHB1

    Plasmid
    #204668
    Purpose
    pUC19 containing the LL-FRT-erm-FRT-spacer sequence which serves as template for BarSeq deletion constructs
    Depositor
    Mark Mandel
    Article
    Burgos et al mSystems. 2020 Dec 15;5(6):e00846-20. do
    Insert
    FRT-erm-FRT
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    204668-plasmid-map-sequence-id-422775

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  27. lentiCas9-Blast

    Plasmid
    #52962
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Has Service
    Lentiviral Prep
    Inserts
    Cas9
    Blasticidin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52962-plasmid-map-sequence-id-322376

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  28. LentiGuide Cherry

    Plasmid
    #170510
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Pavel Tolar
    Article
    Newman et al biorXiv 2021.03.02.433398
    Inserts
    S. pyogenes sgRNA cassette
    mCherry
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a and hU6
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170510-plasmid-map-sequence-id-343694

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  31. pDIV643

    Plasmid
    #226182
    Purpose
    Expresses a color marker (uidA) cassette for disruption on KU70 gene in K. phaffii.
    Depositor
    Uffe Mortensen
    Article
    Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi
    Insert
    uidA marker flanked with targeting regions to KU70 gene in K.phaffii
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    TEF1p
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226182-plasmid-map-sequence-id-449156

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  32. pSBbi-TIR1-tTA/Pur

    Plasmid
    #171683
    Purpose
    constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)
    Depositor
    Randy Poon
    Article
    Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.
    Inserts
    tetracycline-controlled transactivator
    Puromycin
    EF-1a promoter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a promoter and RPBSA promoter
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    171683-plasmid-map-sequence-id-338224

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  38. Gfap-Cryab

    Plasmid
    #58947
    Purpose
    hamster Cryab gene under the control of the human GFAP promoter to target expression to astrocytes
    Depositor
    Albee Messing
    Article
    Hagemann et al Hum Mol Genet. 2009 Apr 1;18(7):1190-9.
    Insert
    Cryab
    Use
    Mouse Targeting; Transgene expression
    Tags
    Expression
    Mammalian
    Mutation
    genomic fragment
    Promoter
    human GFAP promoter (position -2163 to +47)
    Available since
    March 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  40. lentiCas9n(D10A)-Blast

    Plasmid
    #63593
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    Cas9
    Blasticidin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, nickase mutant
    Promoter
    EFS-NS
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63593-plasmid-map-sequence-id-181681

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  45. pCEFL

    Plasmid
    #38121
    Depositor
    JS Gutkind
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1
    Available since
    Sept. 7, 2012
    Availability
    Academic Institutions and Nonprofits only
    38121-plasmid-map-sequence-id-48259

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  48. SpRY

    Plasmid
    #198544
    Purpose
    Expresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpRY
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198544-plasmid-map-sequence-id-390296

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Showing: 451 - 500 of 2330 results