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Plasmid#61594PurposeA catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.DepositorInserthSaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianMutationD10A, N580APromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dSaCas9-KRAB-T2A-PuroR
Plasmid#106249PurposeLentiviral vector with puro selection for expression of S. aureus dCas9-KRABDepositorInsertdead S. aureus Cas9 KRAB T2A PuroR
UseCRISPR and LentiviralTagsHA-NLS and NLS-KRABExpressionMammalianMutationD10A and N580APromoterhUbCAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
DTECT - Plasmid for Standard Curve
Plasmid#139333PurposeThis plasmid is used to generate the unique standard curve required to quantify the capture of genomic signature using DTECTDepositorInsertDTECT ligation product
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
MS2-P65-HSF1_GFP
Plasmid#61423PurposeExpresses the MS2-P65-HSF1 activator helper complex with a 2A GFP. 3rd generation lentiviral vector.DepositorInsertMS2(N55K)-P65-HSF1_2A_GFP (HSF1 Human, Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationN55K in MS2PromoterEF1AAvailable sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
lenti MS2-P65-HSF1_Hygro
Plasmid#61426Purposelenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2DepositorInsertMS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationN55K in MS2PromoterEF1AAvailable sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
shRNA_circHUWE1_1
Plasmid#215236PurposeSupression of shcircHUWE1(19,20)_1 expressionDepositorInsertcircHUWE1 shRNA 1 (HUWE1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
shRNA_circHUWE1_2
Plasmid#215235PurposeSupression of shcircHUWE1(19,20)_2 expressionDepositorInsertcircHUWE1 shRNA 2 (HUWE1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-MIB1-IRES-Puro
Plasmid#140240PurposeLentiviral vector expressing flag-tagged MIB1DepositorInsertFlag-MIB1 (MIB1 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_UBA/UIM/ UBM-mut
Plasmid#187154Purposemammalian cell expression of FLAG-HUWE1 L2976A/P2977A/M1328A/F1330A/A1376G/S1382ADepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationL2976A/P2977A/M1328A/F1330A/A1376G/S1382APromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIneoRL-BCL2 3'UTR-mir200c-mut
Plasmid#84598PurposeRenilla Luciferase reporter assay for BCL2 3'UTR with mutations on miR-200c binding siteDepositorInsertBCL2 3'UTR (UTS2R Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMutationMutation on miR-200c binding site on BCL2 3'…PromoterCMVAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIneoRL-TOB1 3'UTR-mir200c-mut
Plasmid#84590PurposeRenilla Luciferase reporter assay for TOB1 3'UTR with mutations on miR-200c binding siteDepositorInsertTOB1 3'UTR (UTS2R Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMutationMutation on miR-200c binding site on TOB1 3'…PromoterCMVAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK616
Plasmid#72460PurposeProduces Treponema denticola ATCC 35405 PurE with an N-terminal His6 tag (H6TdPurE2)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK636
Plasmid#72466PurposeProduces Treponema denticola ATCC 35405 PurE with a TEV protease-cleavable N-terminal His6 tag (H6tTdPurE2)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastbacHTB HA-human PP2A Cα D88N
Plasmid#132633Purposeexpresses HA-human PP2A Cα D88N in Hi5 insect cellDepositorInsertPP2A Cα D88N
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Di-V_7_R3x1_7
Plasmid#45488DepositorInsertDi-V_7_R3x1_7
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMay 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
Di-IV_5_PL2x2_6
Plasmid#45487DepositorInsertDi-IV_5_PL2x2_6
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMay 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
Di-III_14_IF3_14
Plasmid#45486DepositorInsertDi-III_14_IF3_14
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMay 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
Di-II_10_R2x2_769
Plasmid#45485DepositorInsertDi-II_10_R2x2_769
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMay 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
Di-I_5_FR28
Plasmid#45484DepositorInsertDi-I_5_FR28
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMay 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIMAY*
Plasmid#121441PurposeAllelic exchange plasmid with PheS* counter-selection for Staphylococcus aureusDepositorTypeEmpty backboneUseEscherichia coli/staphylococcus aureus shuttle ve…TagsExpressionMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only