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pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

Plasmid

#62723

Purpose

AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has service

AAV1 and AAV5

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Mutation

Chrimson K176R mutant

Promoter

human synapsin promoter

Available since

April 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

68 Articles

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pAct-Cas9

Plasmid

#62209

Purpose

Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.

Depositor

Simon Bullock

Publication

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

cas9

Expression

Insect

Promoter

act5C

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pENTR-ERKKTRClover

Plasmid

#59138

Purpose

ORF encoding ERK KTR mClover flanked by Gateway sequences

Depositor

Markus Covert

Publication

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

ERK Kinase Translocation Reporter (MAPK1 Mouse, Human)

Available since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pMSCV-IRES-Blue FP

Plasmid

#52115

Purpose

a bicistronic IRES-Blue FP containing retroviral vector

Depositor

Dario Vignali

Type

Empty backbone

Use

Retroviral

Tags

IRES-Blue FP

Expression

Mammalian

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA

Plasmid

#50943

Purpose

Bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Publication

Rose et al Science. 2016 Jun 10;352(6291):1319-22.

Insert

mRuby2-P2A-GCaMP6f

Use

AAV

Promoter

hSyn1

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pRK5-EGFP-Tau AP

Plasmid

#46905

Purpose

expresses EGFP tagged Tau AP in mammalian cells

Depositor

Karen Ashe

Publication

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau AP (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

all 14 S/P or T/P amino acid residues (T111, T153…

Promoter

CMV

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMV-STAT6-IRES-Neo

Plasmid

#35482

Depositor

Axel Nohturfft

Insert

STAT6 (STAT6 Human)

Expression

Mammalian

Promoter

CMV

Available since

April 20, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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CMV500 A-CREB

Plasmid

#33371

Depositor

Charles Vinson

Publication

Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

Insert

CREB (Creb1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Dominant Negative (see notes)

Promoter

CMV

Available since

Dec. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-hSyn-hChR2(H134R)-mCherry

Plasmid

#26976

Purpose

AAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitation

Depositor

Karl Deisseroth

Has service

AAV5, AAV8, and AAV9

Insert

hChR2(H134R)

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

H134R

Promoter

hSyn

Available since

Feb. 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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pLM-mCitrine-Sox2

Plasmid

#23242

Depositor

Michel Sadelain

Publication

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

mCitrine_2A_SOX2 (SOX2 synthetic fluorescent protein, Human)

Use

Lentiviral

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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ChR2-2A-Halo

Plasmid

#22047

Purpose

Expression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activity

Depositor

Edward Boyden

Publication

Han et al Front Mol Neurosci. 2009 Aug 27:2:12. do

Insert

ChR2-GFP-2A-Halo-YFP

Tags

GFP and YFP

Expression

Mammalian

Mutation

Note that there is a S147R mut in YFP. This mutat…

Promoter

CMV

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA

Plasmid

#20299

Purpose

Cre-activated AAV expression of mCherry

Depositor

Karl Deisseroth

Has service

AAV9

Insert

mCherry

Use

AAV

Expression

Mammalian

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pCMV6M-Pak1 K299R

Plasmid

#12210

Depositor

Jonathan Chernoff

Publication

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Catalytically inactive Pak1: K299R

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pDONR207 SARS-CoV-2 M

Plasmid

#141274

Purpose

Gateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

M (M SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

6 Articles

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ER-mScarletI

Plasmid

#137805

Purpose

Visualization of the Endoplasmic Reticulum

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

KDEL

Expression

Mammalian

Promoter

CMV

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pDEST-CMV-C-EGFP

Plasmid

#122844

Purpose

C-terminal EGFP tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Publication

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway Cloning

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMSCV-Cas9-2A-GFP-sgRNA

Plasmid

#124889

Purpose

Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector

Depositor

Harvey Lodish

Publication

Li et al Haematologica. 2016 Jun;101(6):e216-9. d

Insert

Cas9

Use

Retroviral

Tags

2A-GFP

Promoter

MSCV

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV.hSynap.iGABASnFR

Plasmid

#112159

Purpose

GABA Sensor

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Has service

AAV1

Insert

iGABASnFR

Use

AAV

Promoter

hSynapsin

Available since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pN1-GCaMP6m-XC

Plasmid

#111543

Purpose

Improved genetic encoded calcium sensor based on GCaMP6m. Also called GCaMP-X

Depositor

Xiaodong Liu

Publication

Yang et al Nat Commun. 2018 Apr 17;9(1):1504. doi:

Insert

GCaMP6m-XC

Expression

Mammalian

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pcDNA3-ADAM17-HA

Plasmid

#65105

Purpose

Expression of HA tagged ADAM17 (TACE) in mammalian cells

Depositor

Axel Ullrich

Publication

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

ADAM17 (ADAM17 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCytERM_mScarlet_N1

Plasmid

#85066

Purpose

In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

CytERM

Tags

mScarlet

Expression

Mammalian

Mutation

aa1-29

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pCW57-MCS1-P2A-MCS2 (RFP)

Plasmid

#80923

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Publication

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Promoter

TRE

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pR26 CAG/GFP Dest

Plasmid

#74281

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

Depositor

Ralf Kuehn

Publication

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Mouse Targeting

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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PMXS-NDI1

Plasmid

#72876

Purpose

expresses NDI1 in mammalian cells

Depositor

David Sabatini

Publication

Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi

Insert

NDI1 (NDI1 Budding Yeast)

Use

Retroviral

Expression

Mammalian

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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pAV5S-F30-2xdBroccoli

Plasmid

#66845

Purpose

Expresses 5S rRNA fused to two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

Depositor

Samie Jaffrey

Publication

Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

Insert

Two dimeric Broccoli aptamers in the F30 scaffold

Tags

5S rRNA

Expression

Mammalian

Promoter

5S

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET28a-EGFP-CNA35

Plasmid

#61603

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with EGFP

Depositor

Maarten Merkx

Publication

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

EGFP and CNA35

Tags

His tags

Expression

Bacterial

Promoter

T7

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter

Plasmid

#61762

Purpose

Dual Color betaGlobin reporter to study splicing kinetics in vivo

Depositor

Daniel Larson

Publication

Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

Insert

b-Globin

Tags

24 X MS2 hairpin cassette in 3'UTR of b-Glob…

Expression

Mammalian

Promoter

Dox inducible Tet-ON promoter

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CibN-dCas9-CibN

Plasmid

#60553

Purpose

Expresses CibN-dCas9-CibN in mammalian cells

Depositor

Charles Gersbach

Publication

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

dCas9-CibN

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pDisplay-pHuji

Plasmid

#61556

Purpose

Red fluorescent protein based pH indicator in pDisplay vector

Depositor

Robert Campbell

Publication

Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

Insert

pHuji

Expression

Mammalian

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pcDNA4TO-24xGCN4_v4-sfGFP

Plasmid

#61058

Purpose

A direct fusion of sfGFP to the 24xGCN4_v4 peptide array

Depositor

Ron Vale

Publication

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

24xGCN4_v4 peptide array and sfGFP

Expression

Mammalian

Available since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only

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