165,951 results

1. pENTR221-Renilla

   Plasmid

   #79496

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Renilla Luciferase CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   Renilla Luciferase
   

   Use

   Multisite gateway entry clone

   Promoter

   no Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGWB441

   Plasmid

   #74827

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with eYFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. HLTV-hTRIM21

   Plasmid

   #104973

   Purpose

   Purification of His-Lipoyl-hTRIM21 protein from bacteria

   Depositor

   Leo James

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   Tripartite Motif containing 21 (TRIM21 Human)
   

   Tags

   His-Lipoyl

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJM1-Tmem192-mRFP-3xHA

   Plasmid

   #134631

   Purpose

   bait for affinity-isolation of lysosomes

   Depositor

   Roberto Zoncu

   Article

   Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   3xHA, TEV, and mRFP

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1_RFP

   Plasmid

   #206177

   Purpose

   mammalian expression of RFP

   Depositor

   Christopher Goodnow

   Article

   Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

   Insert

   RFP
   

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PiggyBac PlayBack

   Plasmid

   #203312

   Purpose

   To all for PiggyBac gene insertion into Genome

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-EWSR1::WT1(-KTS)

   Plasmid

   #220565

   Purpose

   Lentiviral expression of human EWSR1::WT1(-KTS)

   Depositor

   Sean Lee

   Article

   Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

   Insert

   EWSR1::WT1(-KTS) (EWSR1, WT1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO-Tet-On-YAP1-shRNA1

   Plasmid

   #176846

   Purpose

   lentiviral vector for doxycycline-inducible human YAP1 knockdown

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   YAP1-Sh1 (YAP1 Human)
   

   Use

   Lentiviral

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-SpCas9-GFP-U6-gRNA

   Plasmid

   #79144

   Purpose

   All-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSC

   Depositor

   Jizhong Zou

   Article

   Stoehr et al JCI Insight. 2019 May 21;5. pii: 128496.

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTD103luxI_sfGFP

    Plasmid

    #48885

    Purpose

    Positive feedback plasmid from oscillator

    Depositor

    Jeff Hasty

    Article

    Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

    Insert

    LuxI, sfGFP, and LuxR
    

    Use

    Synthetic Biology

    Tags

    ssrA tag (AANDENYALAA)

    Expression

    Bacterial

    Promoter

    pLux

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCh-Drp1

    Plasmid

    #49152

    Purpose

    mammalian expression of Drp1 fused to mCherry

    Depositor

    Gia Voeltz

    Article

    Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

    Insert

    Drp1 (DNM1L Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. LYN11-FRB-mcherry

    Plasmid

    #38004

    Depositor

    Robin Irvine

    Article

    Hammond et al Science. 2012 Jun 21.

    Inserts

    LYN (LYN Human)
    MTOR (MTOR Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT3-EF1aH

    Plasmid

    #180149

    Purpose

    This plasmid is an empty vector used as the control for other plasmids which are in pT3-EF1aH backbone. It does not have any restriction site for cloning.

    Depositor

    Xin Chen

    Article

    Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-HA-14-3-3 gamma

    Plasmid

    #13274

    Depositor

    Michael Yaffe

    Insert

    14-3-3 gamma (Ywhag Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. mEGFP-N1-YAP

    Plasmid

    #166457

    Purpose

    Expresses fusion of mEGFP and YAP

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP, YAP (YAP1 Human)
    

    Expression

    Mammalian

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

    Plasmid

    #221235

    Purpose

    Tet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA
    

    Use

    Lentiviral

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti6-GFP

    Plasmid

    #35637

    Depositor

    Daniel Haber

    Article

    Singh et al Cell. 2012 Feb 17;148(4):639-50.

    Insert

    GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pT7II-2N4Rtau

    Plasmid

    #177653

    Purpose

    Recombinant protein synthesis of human Tau

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    Tau (2N4R) (MAPT Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpySwitch

    Plasmid

    #184225

    Purpose

    Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.

    Depositor

    Mark Howarth

    Article

    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

    Insert

    SpySwitch
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    E77A mutation prevents isopeptide bond formation,…

    Promoter

    T7

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cas9-PCV

    Plasmid

    #123644

    Purpose

    Expresses Cas9 with a C-terminal HUH-tag called PCV2 in E. coli

    Depositor

    Wendy Gordon

    Article

    Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

    Inserts

    PCV2 (ORF1 )
    Cas9
    

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Mutation

    2-116

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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