4,132 results

1. MSCVhygro-F-G9a

   Plasmid

   #41721

   Purpose

   Retroviral expression of human G9a in mammalian cells

   Depositor

   Kai Ge

   Article

   Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

   Insert

   G9a (EHMT2 Human)
   

   Use

   Retroviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-Brainbow-2.1 R

   Plasmid

   #18723

   Depositor

   Joshua Sanes

   Article

   Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

   Insert

   hrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-NFAT1(4-460)-GFP

   Plasmid

   #11107

   Purpose

   Expresses mouse NFAT1 (amino acids 4-460) with HA and GFP tags in mammalian cells

   Depositor

   Anjana Rao

   Article

   Aramburu et al Science. 1999 Sep 24. 285(5436):2129-33.

   Insert

   NFAT1 (Nfatc2 Mouse)
   

   Use

   Tags

   3x HA and GFP

   Expression

   Mammalian

   Mutation

   Contains amino acids 4-460 of NFAT1; L78P compare…

   Promoter

   CMV

   Available since

   May 31, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. PT2/C-Luc//PGK-SB13

   Plasmid

   #20207

   Depositor

   John Ohlfest

   Article

   Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

   Insert

   Flirefly luciferase/Sb transposase
   

   Use

   Transposon

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. HIR WT

   Plasmid

   #24049

   Depositor

   Frederick Stanley

   Article

   Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

   Insert

   Insulin Receptor (INSR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. mTurquoise2-N1

   Plasmid

   #54843

   Purpose

   Localization: N1 Cloning Vector, Excitation: 434, Emission: 474

   Depositor

   Dorus Gadella, Michael Davidson

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Type

   Empty backbone

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. LRG (Lenti_sgRNA_EFS_GFP)

   Plasmid

   #65656

   Purpose

   Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

   Depositor

   Christopher Vakoc

   Article

   Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter-driven sgRNA expression and EFS promo…

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYLCRISPR/Cas9Pubi-H

   Plasmid

   #66187

   Purpose

   expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selection

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Plant

   Mutation

   plant-codon optimized with higher GC contents (62…

   Promoter

   maize ubiquitin promoter (Pubi)

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMV306hsp

   Plasmid

   #26155

   Depositor

   Siouxsie Wiles, Brian Robertson

   Article

   Andreu et al PLoS One. 2010 May 24;5(5):e10777.

   Insert

   hsp60 promoter
   

   Use

   Mycobacteria integrating vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe-puro largeTcDNA

    Plasmid

    #14088

    Depositor

    William Hahn

    Article

    Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

    Insert

    Large T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

    Plasmid

    #64216

    Purpose

    Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA targeting ROSA26-1
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-ATR WT

    Plasmid

    #31611

    Depositor

    Aziz Sancar

    Article

    Jiang et al Mol Cell Biol. 2006 Jan . 26(1):39-49.

    Insert

    ATR (ATR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRE-KRAB-dCas9-IRES-BFP

    Plasmid

    #85449

    Purpose

    Lentiviral vector expressing a KRAB-dCas9 fusion protein and BFP

    Depositor

    Eric Lander

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Insert

    KRAB-dCas9-IRES-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA Tag, and KRAB domain

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lyn-tailed mCherry-SEpHluorin

    Plasmid

    #32002

    Depositor

    Sergio Grinstein

    Article

    Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

    Insert

    Membrane-targeting sequence of Lyn kinase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. p6XHis_NLS-SaCas9

    Plasmid

    #101086

    Purpose

    Express SaCas9 in bacteria with a 6xHis tag for purification

    Depositor

    Rick Tarleton

    Article

    Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

    Insert

    CRISPR-associated protein Cas9/Csn1
    

    Use

    CRISPR

    Tags

    TrxA-6xHis-STag

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-Rubicon

    Plasmid

    #28022

    Depositor

    Qing Zhong

    Article

    Sun et al J Biol Chem. 2011 Jan 7. 286(1):185-91.

    Insert

    Rubicon (RUBCN Human)
    

    Use

    Tags

    3xFlag and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-VSVL

    Plasmid

    #64085

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain polymerase gene

    Depositor

    Ian Wickersham

    Insert

    VSV polymerase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRDA_052

    Plasmid

    #136474

    Purpose

    Lentiviral expression of AsCas12a gRNA

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    Puromycin resistance
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCSCMV:tdTomato

    Plasmid

    #30530

    Purpose

    mammalian expression of tdTomato controlled by the ubiquitously active CMV promoter.

    Depositor

    Gerhart Ryffel

    Article

    Waldner et al BMC Dev Biol. 2009 Jun 23;9:37. doi: 10.

    Has Service

    Cloning Grade DNA

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R415H

    Promoter

    Available since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDestTol2pA2-U6:gRNA

    Plasmid

    #63157

    Purpose

    Destination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Type

    Empty backbone

    Use

    CRISPR; Tol2 destination vector for gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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