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We narrowed to 5,067 results for: codon optimized

Showing: 4001 - 4040 of 5067 results

  9. pCC_07 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139092
    Purpose
    Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dxCas9 3.7-VPR
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139092-plasmid-map-sequence-id-273889

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  11. pCAT526

    Plasmid
    #180513
    Purpose
    Plasmid expressing mammalian codon optimized wt PlmCasX, mNeonGreen, sgRNAv1 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv1
    PlmCasX-2A-mNeonGreen
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180513-plasmid-map-sequence-id-359728

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  21. pAM4663

    Plasmid
    #85126
    Purpose
    NSIII -Cm-PkaiB-luc+ (firefly) vector constructed by seamless cloning with Cm casette codon optimized for S7942. PkaiB-luc+ amplified from pAM2105.
    Depositor
    Susan Golden
    Article
    Chang et al Science. 2015 Jul 17;349(6245):324-8. do
    Insert
    PkaiB-luciferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85126-plasmid-map-sequence-id-180127

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  25. pYPQ239-RVR

    Plasmid
    #108863
    Purpose
    FnCpf1 Gateway entry plasmid with N623R, K629R and N633R mutations
    Depositor
    Yiping Qi
    Article
    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do
    Insert
    FnCpf1-RVR (FnCpf1 with N623R, K629R and N633R mutations)
    Use
    CRISPR; Gateway compatible cpf1 entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    N623R, K629R and N633R, Cpf1 is rice codon optimi…
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    108863-plasmid-map-sequence-id-274167

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  30. E. coli Lig-N-HiBiT

    Plasmid
    #215437
    Purpose
    Bacterially expressed E. coli Lig-N-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    NAD-dependent DNA ligase LigA [Escherichia coli], N-HiBiT, N-10XHis.Thrombin
    Use
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 gene…
    Promoter
    T7 Promotor
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215437-plasmid-map-sequence-id-428719

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  31. E. coli Lig-C-HiBiT

    Plasmid
    #215438
    Purpose
    Bacterially expressed E. coli Lig-C-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    NAD-dependent DNA ligase LigA [Escherichia coli], C-HiBiT, C-10XHis.Thrombin
    Use
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 gene…
    Promoter
    T7 Promotor
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215438-plasmid-map-sequence-id-428720

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  32. T7 Lig-C-HiBiT

    Plasmid
    #215441
    Purpose
    Bacterially expressed T7 Lig-C-HiBit for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Escherichia phage T7 DNA Ligase, C-terminal HiBiT, C-terminal 10XHis.Thrombin tag
    Use
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 gene…
    Promoter
    T7 Promotor
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215441-plasmid-map-sequence-id-428723

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  33. *MtbCM-bio-His

    Plasmid
    #186027
    Purpose
    Expresses secreted Mycobacterium tuberculosis (M. tb) chorismate mutase (CM) containing a C-terminal biotinylation site and His tag
    Depositor
    James Inglese
    Article
    van Neer et al ACS Infect Dis. 2025 Mar 14;11(3):703-71
    Insert
    Rv1885c Chorismate Mutase
    Use
    Tags
    Avi-tag for in vitro biotinylation and His-tag wi…
    Expression
    Bacterial
    Mutation
    Codon optimization for expression in BL21DE3
    Promoter
    T7 Promotor
    Available since
    March 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    186027-plasmid-map-sequence-id-370660

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  35. TadA-8e-eIscBn-C-HMG-D

    Plasmid
    #227145
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain in C terminus driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-TadA-8e-linker-nOgeuIscB-v3-linker-HMG-D-bpNLS
    Use
    Tags
    Expression
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227145-plasmid-map-sequence-id-453986

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  36. eIscB-D

    Plasmid
    #227017
    Purpose
    Vector encoding human codon-optimized enhanced-activity OgeuIscB fusion with HMD-D domain and extra SV40 NLS driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    SV40 NLS-HMG-D-linker-OgeuIscB-v3-SV40-nucleoplasmin NLS
    Use
    Tags
    Expression
    Mutation
    D96R/E84R/V159R
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227017-plasmid-map-sequence-id-453985

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  37. TadA-8e-eIscBn

    Plasmid
    #227018
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-TadA-8e-linker-nOgeuIscB-v3-bpNLS
    Use
    Tags
    Expression
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227018-plasmid-map-sequence-id-453983

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  38. hA3A*-IscBn

    Plasmid
    #227146
    Purpose
    Vector encoding human codon-optimized nOgeuIscB fusion with two copies of UGI and hA3A* variant driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-hA3A*-linker-nOgeuIscB-nucleoplasmin NLS-2XUGI-bpNLS
    Use
    Tags
    Expression
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227146-plasmid-map-sequence-id-453980

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  39. TadA-8e-eIscBn-N-HMG-D

    Plasmid
    #227143
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain in N terminus driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-HMG-D-linker-TadA-8e-linker-nOgeuIscB-v3-bpNLS
    Use
    Tags
    Expression
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227143-plasmid-map-sequence-id-453972

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Showing: 4001 - 4040 of 5067 results