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We narrowed to 15,548 results for: pET

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Showing: 5001 - 5050 of 15548 results

  5. pUD-MSP1D1

    Plasmid
    #161608
    Purpose
    Bacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do
    Insert
    UD-MSP1D1
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    mutant of MSP1D1; all tryptophans and tyrosines w…
    Promoter
    T7
    Available Since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161608-plasmid-map-sequence-id-317655

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  10. pD-MSP1D1

    Plasmid
    #161607
    Purpose
    Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do
    Insert
    D-MSP1D1
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    mutant of MSP1D1; all tryptophans were replaced b…
    Promoter
    T7
    Available Since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161607-plasmid-map-sequence-id-317653

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  20. pUB-MSP1D1

    Plasmid
    #161609
    Purpose
    Bacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do
    Insert
    UD-MSP1D1
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    mutant of MSP1D1 ( Q138W, L181W, Y236W)
    Promoter
    T7
    Available Since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161609-plasmid-map-sequence-id-317666

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  23. pAGD289

    Plasmid
    #228113
    Purpose
    pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter
    Depositor
    Seth Shipman
    Article
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Kva1 retron, ncRNA with 90 bp rpoB donor
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    228113-plasmid-map-sequence-id-455374

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  25. pCoofy48

    Plasmid
    #55186
    Purpose
    Bacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Tags
    6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    55186-plasmid-map-sequence-id-464535

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  29. pHIT179

    Plasmid
    #204510
    Purpose
    Bacterial expression of CSR-1a with 6xHis and maltose binding protein tags for in vitro assays
    Depositor
    Yuji Kohara
    Article
    Aoki et al EMBO J. 2007 Dec 12. 26(24):5007-19.
    Insert
    csr-1a (C. elegans) (csr-1 Nematode)
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Promoter
    T7lac
    Available Since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    204510-plasmid-map-sequence-id-403822

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  31. pMH-macroHC

    Plasmid
    #82337
    Purpose
    Provides a C-terminal macro domain and 6xHis tag for expression in E. coli
    Depositor
    Michael Hothorn
    Article
    Wild et al Protein Sci. 2016 Oct 24. doi: 10.1002/p
    Insert
    SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
    Tags
    6xHis non-cleavable and macro domain of human his…
    Expression
    Bacterial
    Mutation
    Truncated protein, amino acids 1-178
    Available Since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    82337-plasmid-map-sequence-id-155830

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  34. pPD3C

    Plasmid
    #86971
    Purpose
    Expresses PCNA3 protein fused phosphite dehydrogenase and P450cam in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA3 fused to phosphite dehydrogenase and P450cam
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, N106R in PCNA3, silent mutat…
    Promoter
    T7 promoter
    Available Since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    86971-plasmid-map-sequence-id-173928

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  37. pMW96_6xHis-Sumo-TEV-mNG

    Plasmid
    #253320
    Purpose
    bacterial expression and purification of mNG
    Depositor
    Kristen Verhey
    Insert
    mNG
    Tags
    His-SUMO
    Expression
    Bacterial
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    253320-plasmid-map-sequence-id-504568

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  38. pTE5605

    Plasmid
    #250512
    Purpose
    Mutant of pSecYEG; impaired in translocation and insertion; cell-free expression of functional SecYEG
    Depositor
    Tobias Erb
    Article
    Meier et al bioRxiv 2025.12.09.688994
    Insert
    T7-secY(P84L)EG
    Expression
    Bacterial
    Mutation
    P84L
    Promoter
    T7
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250512-plasmid-map-sequence-id-498896

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  39. pTE5627

    Plasmid
    #250516
    Purpose
    Super-active translocator mutant of pSecYEG; cell-free expression of functional SecYEG
    Depositor
    Tobias Erb
    Article
    Meier et al bioRxiv 2025.12.09.688994
    Insert
    T7-secY(I408G)EG
    Expression
    Bacterial
    Mutation
    I408G
    Promoter
    T7
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250516-plasmid-map-sequence-id-498862

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  40. pTE5639

    Plasmid
    #250519
    Purpose
    Superactive inserter mutant of pSecYEG; cell-free expression of functional SecYEG
    Depositor
    Tobias Erb
    Article
    Meier et al bioRxiv 2025.12.09.688994
    Insert
    T7-secY(T166L)EG
    Expression
    Bacterial
    Mutation
    T166L
    Promoter
    T7
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250519-plasmid-map-sequence-id-498895

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  42. EGF-A-SpyTag

    Plasmid
    #246944
    Purpose
    Expresses multifunctional construct for YES-gated release of Epidermal Growth Factor (EGF) from materials in response to sortase eSrtA(2A9) treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag
    Expression
    Bacterial
    Available Since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246944-plasmid-map-sequence-id-489549

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  43. bla-A OR T-SpyTag

    Plasmid
    #246945
    Purpose
    Expresses multifunctional construct for OR-gated release of beta-lactamase (bla) from materials in response to sortase eSrtA(2A9) OR TEV treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag
    Expression
    Bacterial
    Available Since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246945-plasmid-map-sequence-id-489550

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  44. mCherry-S-SpyTag

    Plasmid
    #243786
    Purpose
    Expresses multifunctional construct enabling the YES-gated release of mCherry from materials in response to sortase eSrtA(4S9) treatment. Construct contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/
    Insert
    mCherry
    Tags
    6x His tag
    Expression
    Bacterial
    Available Since
    Oct. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    243786-plasmid-map-sequence-id-486522

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Showing: 5001 - 5050 of 15548 results