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pRK7-myr-RSK1

Plasmid

#8997

Depositor

John Blenis

Article

Richards et al Mol Cell Biol. 2001 Nov . 21(21):7470-80

Insert

RSK (RPS6KA1 Chicken)

Tags

myr

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pGEX-HiGlpG

Plasmid

#158538

Purpose

Bacterial expression of rhomboid protease HiGlpG

Depositor

Sinisa Urban

Article

Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

Insert

HiGlpG

Tags

GST tag

Expression

Bacterial

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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p2Lox

Plasmid

#34635

Depositor

Michael Kyba

Article

Iacovino et al Stem Cells. 2011 Oct;29(10):1580-8.

Type

Empty backbone

Tags

EGFP

Expression

Bacterial

Promoter

PGK promoter

Available Since

Feb. 7, 2012

Availability

Academic Institutions and Nonprofits only
pCSGreen

Plasmid

#22412

Depositor

Nathan Lawson

Article

Lawson et al Development. 2001 Oct . 128(19):3675-83.

Insert

EGFP (eGFP )

Use

Mrna expression in zebrafish

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pFA6a- mCFP-kanMX6

Plasmid

#105145

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mCFP-kanMX6

Tags

mCFP

Expression

Bacterial and Yeast

Mutation

A206K

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pJH4.67

Plasmid

#26948

Depositor

Geraldine Seydoux

Article

Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Insert

gamma-tubulin (tbg-1 Nematode)

Tags

GFP

Expression

Worm

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pET28a-SUMO-SpyTag003

Plasmid

#216282

Purpose

Expresses SUMO with a C-terminal SpyTag003 in bacterial cells

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

SUMO-SpyTag003

Tags

His6 and SpyTag003

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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EC.SurA.pTYB1.a21-428.wt

Plasmid

#12606

Depositor

David McKay

Article

Bitto et al Structure. 2002 Nov . 10(11):1489-98.

Insert

Escherichia coli surA (surA E. coli)

Tags

intein

Expression

Bacterial

Mutation

wild type

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Lunatic Fringe

Plasmid

#24962

Depositor

Edward De Robertis

Article

Pera et al Int J Dev Biol. 2005 . 49(7):781-96.

Insert

Lunatic Fringe (lfng Frog)

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pCS2 xenopus sizzled HA

Plasmid

#24683

Depositor

Edward De Robertis

Article

Lee et al Cell. 2006 Jan 13. 124(1):147-59.

Insert

Sizzled (szl Frog)

Tags

HA

Expression

Mammalian

Available Since

July 22, 2010

Availability

Academic Institutions and Nonprofits only
TAL2084

Plasmid

#39452

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-48-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
U6-TM2Emp-6T_iBlue

Plasmid

#200235

Purpose

Backbone for cloning short RNA triggers that activate iSBH-sgRNAs

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-170nDelta13V

Plasmid

#18018

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16F Delta13

Tags

ECFP and Venus

Expression

Mammalian

Mutation

WT binding protein (no F16A)

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pEF/myc/ER FLIPglu-600uDelta13V

Plasmid

#18020

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16A Delta13

Tags

ECFP and Venus

Expression

Mammalian

Mutation

F16A in binding protein

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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TAL2052

Plasmid

#39410

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-27-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
TAL2053

Plasmid

#39411

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-27-Right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
Reb_82 aka PAS623

Plasmid

#71228

Purpose

Reb_1 with RebB(S82P, D88G)

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

none

Expression

Bacterial

Mutation

RebB(S82P, D88G), RebA (G53A)

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
TAL2085

Plasmid

#39453

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-48-Right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
TAL2073

Plasmid

#39443

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-43-Right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
pEnt L1L3 EF1a-tTA-3

Plasmid

#24415

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Type

Empty backbone

Use

Entry vector

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only

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pWT055g

Plasmid

#96864

Purpose

sgRNA-HEK3

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

sgRNA-HEK3

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL2081

Plasmid

#39451

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-47-Right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
TAL2080

Plasmid

#39450

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-47-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
TAL2060

Plasmid

#39418

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-31-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
TAL2061

Plasmid

#39419

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-31-Right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
TAL2033

Plasmid

#39391

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-17-Right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
TAL2032

Plasmid

#39390

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-17-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
TAL2093

Plasmid

#39427

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-35-Right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
TAL2092

Plasmid

#39426

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-35-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
TAL2072

Plasmid

#39442

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-43-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pFKBPmCherry-Akt2(KD)

Plasmid

#86598

Purpose

Expresses recruitable mCherry-tagged kinase domain of human Akt2(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

FKBP-mCherry-Akt2(KD)

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL2095

Plasmid

#39429

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-36-Right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
TAL2094

Plasmid

#39428

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-36-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
pSEYC68-HA-TOR2 (pAS21)

Plasmid

#39207

Depositor

Michael Hall

Article

Kunz et al J Biol Chem. 2000 Nov 24;275(47):37011-2

Insert

TOR2 (TOR2 Budding Yeast)

Tags

HA

Expression

Yeast

Promoter

GAL

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
pKP660

Plasmid

#160812

Purpose

Expression of ExbB, ExbD proteins

Depositor

Kathleen Postle

Article

Ollis et al Mol Microbiol. 2009 Aug;73(3):466-81. do

Insert

exbB, exbD

Expression

Bacterial

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SNX9

Plasmid

#171943

Purpose

Lentiviral plasmid to express mouse SNX9 in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

SNX9

Use

Lentiviral

Expression

Mammalian

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-alpha2-chimaerin

Plasmid

#59316

Purpose

Expression plasmid for GFP-tagged alpha2-chimaerin (GFP at N-terminus of alpha2-chimaerin)

Depositor

Peter Scheiffele

Article

Beg et al Neuron. 2007 Sep 6;55(5):768-78.

Insert

alpha2-chimaerin

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK421

Plasmid

#114688

Purpose

RFP-Nano tagging at NuMA's C-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

RFP-Nano

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pWT055e

Plasmid

#96866

Purpose

sgRNA-EMX1

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

sgRNA-EMX1

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
RGG-NanoLuc-RGG

Plasmid

#176642

Purpose

NanoLuc enzyme fused to LAF-1 RGG domain

Depositor

Daniel Hammer

Article

Guan et al Biochemistry. 2021 Oct 14. doi: 10.1021/

Insert

RGG-NanoLuc-RGG

Expression

Bacterial

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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