We narrowed to 8,775 results for: construct
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Plasmid#123902PurposeEncodes the GFP dropout cassette for multi-TU destination vectors as a type 234 part to be used in the MTK systemDepositorInsertGFP dropout for multiTU destination vectors
ExpressionMammalianAvailable SinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK2_007
Plasmid#123702PurposeEncodes human pEF1alpha as a Type 2 part to be used in the MTK systemDepositorInsertpEF1a
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
LLP109 pEF1a-gRNA-GFP
Plasmid#100935PurposeGFP, puromycin and gRNA scaffold driven by EF1a promoterDepositorInsertgRNA scaffold
UseGrna scaffoldExpressionMammalianAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
MTK4_003
Plasmid#123812PurposeEncodes rabbit beta-globin polyadenylation signal as a Type 4 part to be used in the MTK systemDepositorInsertrglb pA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKMV-VioB
Plasmid#65348Purposesysthesis of vioB gene in the violacein pathwayDepositorInsertvioB
UseSynthetic BiologyAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-dsRed-L3 #BGV203
Plasmid#48973PurposeSupplies the selection marker (dsRed) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInsertdsRed
UseGateway cloning vectorTagsIRES (strong)PromoternoneAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-NORAD
Plasmid#196086PurposeInducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting lncRNA NORAD.DepositorInsertgRNA targeting lncRNA NORAD
UseCRISPRExpressionMammalianMutationCloned using SapI sites - destroyed after inserti…Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-iBlast-L3 #BGV204
Plasmid#48982PurposeSupplies the selection marker (blasticidin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInsertblasticidin-S deaminase
UseGateway cloning vectorTagsIRES (weak)PromoternaAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pLEG(R1-R4) #BGV057
Plasmid#48957PurposeLentiviral Destination vector containing AttR1-AttR4 site. Use with 4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4)DepositorTypeEmpty backboneUseLentiviralPromoterCMVAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag OSM
Plasmid#12191DepositorAvailable SinceOct. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
KTK_354
Plasmid#180525PurposeKTK compatible Intergration/suicide plasmid. Contains a LacZ dropout cassette flanked by regions homolgous to the arsenic resistance cassete.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEG R3-miRNA(ccdB)-L4 #BGV228
Plasmid#48953PurposeTo create an AttR3-AttL4 flanked Entry vector encoding a miR30-embedded shRNA to knockdown targeted gene expression. (This corresponds to a module 4 plasmid).DepositorTypeEmpty backboneUseGateway cloning vectorPromoternaAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-iHygro-L3 #BGV206
Plasmid#48985PurposeSupplies the selection marker (hygromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInserthygromycin phosphotransferase
UseGateway cloning vectorTagsIRES (weak)PromoternaAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
MTK3_010
Plasmid#123725PurposeEncodes Tet-On 3G transactivator protein as a Type 3 part to be used in the MTK systemDepositorInsertTetOn3G
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM5082
Plasmid#85121PurposePkaiB-kaiC-ecfp expressed from NSII-Gm vector constructed by seamless assembly. Fusion expressed as full length fusion protein at WT levels but is 5hr long period. Used only for co-localization expDepositorInsertPkaiB-KaiC-ECFP
TagsECFPExpressionBacterialAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
POT2-RFP
Plasmid#65318Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
MTK8b_004
Plasmid#123892PurposeEncodes the 3rd gen. 5' HIV LTR cassette as a type 8b part to be used in the MTK systemDepositorInsert5' HIV LTR (3rd gen.)
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_032
Plasmid#123995PurposeEncodes the spCas9 sgRNA1 hAAVS1 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA1 -hAAVS1
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-A10
Plasmid#196085PurposeInducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.DepositorInsertgRNA targeting Adam10
UseCRISPRExpressionMammalianMutationCloned using SapI sites - destroyed after inserti…Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTK0_031
Plasmid#123970PurposeEncodes the BxBI-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK systemDepositorInsertBxBI-hygro-mRuby2PEST landing
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK7_014
Plasmid#123881PurposeEncodes the extended 3rd gen. 3' HIV LTR cassette as a type 7 part to be used in the MTK systemDepositorInsert3' HIV LTR (3
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3b_031
Plasmid#123809PurposeEncodes Kruppel Associated Box repression domain fused to methyl CpG binding protein 2 as a Type 3b part to be used in the MTK systemDepositorInsertKRAB:meCP2
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_033
Plasmid#123996PurposeEncodes the spCas9 sgRNA1 hAAVS1 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA2 -hAAVS1
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_034
Plasmid#123997PurposeEncodes the spCas9 sgRNA1 hAAVS1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA3 -hAAVS1
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKMV-VioE
Plasmid#65351Purposesysthesis of vioE gene in the violacein pathwayDepositorInsertvioE
UseSynthetic BiologyAvailable SinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
POT4-RFP
Plasmid#65320Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
MTK5_012
Plasmid#123856PurposeEncodes ConRE' and the human A1 insulator as a Type 5 part to be used in the MTK systemDepositorInsertConnector RE'-A1 Insulator
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKMV-VioA
Plasmid#65347Purposesysthesis of vioA gene in the violacein pathwayDepositorInsertvioA
UseSynthetic BiologyAvailable SinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-RiboJ-LacZa-T7
Plasmid#173224PurposepFGC-T7-RJBB to clone genes by Golden Gate (BsaI). To be used in cell-free systemsDepositorTypeEmpty backboneExpressionBacterialPromoterT7Available SinceMarch 9, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
CFP-OSM
Plasmid#12190DepositorAvailable SinceOct. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
MTK4a_020
Plasmid#123833PurposeEncodes the d4 PEST tag as a Type 4a part to be used in the MTK systemDepositorInsertd4PEST tag
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK2_004
Plasmid#123699PurposeEncodes mouse pPGK1 as a Type 2 part to be used in the MTK systemDepositorInsertpPGK1
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK0_030
Plasmid#123969PurposeEncodes the phiC31-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK systemDepositorInsertphiC31-hygro-mRuby2PEST landing
ExpressionMammalianAvailable SinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK7_013
Plasmid#123880PurposeEncodes the 5' 500 bp hAAVS1 locus homology arm as a type 7 part to be used in the MTK systemDepositorInsertRA_hAAVS1
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK0_014
Plasmid#123929PurposeEncodes the BxBI attB GFP dropout destination vector as a type 0 part to be used in the MTK systemDepositorInsertBxBIattB destination vector
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT7-RiboJ-mScarlet-T7
Plasmid#173227PurposeEncodes mScarlet fluorescent protein. To be used in cell-free systemDepositorInsertmScarlet
UseCell-free systemExpressionBacterialPromoterT7Available SinceMarch 9, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MTK4a_019
Plasmid#123832PurposeEncodes the d2 PEST tag as a Type 4a part to be used in the MTK systemDepositorInsertd2PEST tag
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK678_002
Plasmid#123923PurposeEncodes the TU GFP dropout cassette with Ampicillin resistance and bacterial artificial chromosome as a type 678 part to be used in the MTK systemDepositorInsertBAC-AmpR
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0510
Plasmid#124001PurposeEncodes pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pEF1a-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only