We narrowed to 34,416 results for: CaS;

1. A3Bi-Cas9n-UGI

   Plasmid

   #119135

   Purpose

   Base editor made from full-length APOBEC3B with an L1 intron to prevent self-restriction

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3B L1 intron-Cas9 nickase-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   L1 intron insertion

   Promoter

   CMV

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pX330_Actb sgRNA / hSpCas9

   Plasmid

   #172832

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX330-NL-DHFR-SpCas9

   Plasmid

   #124522

   Purpose

   Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

   Insert

   NL-DHFR-SpCas9
   

   Use

   AAV

   Tags

   Destabilized domain of E.coli dihydrofolate reduc…

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pT3TS-nCas9n-A71

   Plasmid

   #125644

   Purpose

   micro-injection of poly-adenylated nCas9n RNA

   Depositor

   Jason Berman

   Article

   Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

   Insert

   nCas9n
   

   Use

   Rna in vitro transcription vector

   Tags

   Expression

   Mutation

   Promoter

   T3

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSpCas9(BB)-2A-GFP-G3BP2(1)

   Plasmid

   #136060

   Purpose

   G3BP2 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (TCATACTAAAATTCGTCATG)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP2 (G3BP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX165-Sniper-Cas9

   Plasmid

   #140560

   Purpose

   Expresses Sniper Cas9 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mutation

   F539S M763I K890N

   Promoter

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pX459-HypaCas9-ANLN_sgRNA

   Plasmid

   #183874

   Purpose

   pX459V2.0-HypaCas9 plasmid with ANLN sgRNA for N-terminal tagging of anillin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ANLN sgRNA spacer (ANLN Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCaST-elav-VP16AD

   Plasmid

   #15308

   Depositor

   Benjamin White

   Article

   Luan et al Neuron. 2006 Nov 9. 52(3):425-36.

   Insert

   VP16AD-ZIP+
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. dCas9-CBP HAT

   Plasmid

   #179547

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SauCas9 EMX1-pegRNA

    Plasmid

    #169857

    Purpose

    SauCas9 pegRNA for EMX1

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SauCas9 EMX1-pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-CibN-dCas9

    Plasmid

    #60551

    Purpose

    Expresses CibN-dCas9 in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    CibN-dCas9
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    inactivating Cas9 mutations D10A and H840A

    Promoter

    CMV

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX165-Cas9-HF1

    Plasmid

    #140565

    Purpose

    Expresses Cas9-HF1 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mutation

    N497A R661A Q695A Q926A

    Promoter

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SP6-hCas9-Ce-mRNA

    Plasmid

    #47911

    Purpose

    In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.

    Depositor

    Paul Sternberg

    Article

    Chiu et al Genetics. 2013 Aug 26.

    Insert

    hCas9 with C. elegans 5' and 3' UTRs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LentiCas9-sgCh2-2-Blast

    Plasmid

    #199645

    Purpose

    Expresses Cas9 and sgRNA control guide targeting intergenic region

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAC1806_pmax-FKBP-dCasRx

    Plasmid

    #118639

    Purpose

    Transient transfection; Expresses FKBP-dCasRx; CAGGS promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    FKBP-dCasRx
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMV-AsCas12f-v4.1

    Plasmid

    #204636

    Purpose

    Expression of AsCas12f v4.1 in mammalian cells

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    AsCas12f-v4.1
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    D169K, N199K, N328G, D364R

    Promoter

    CMV

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RCAS GH6 (CC#152)

    Plasmid

    #15137

    Depositor

    Connie Cepko

    Article

    Schulte et al Development. 2000 Dec . 127(23):5033-45.

    Insert

    GH6 (HMX1 Chicken)
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 15, 2007

    Availability

    Academic Institutions and Nonprofits only

18. RCAS(A) mPea3 FL (CT#1119)

    Plasmid

    #13908

    Depositor

    Cliff Tabin

    Article

    Brent et al Development. 2004 Aug . 131(16):3885-96.

    Insert

    Pea3 (Etv4 Mouse)
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pENTR2B-dCas9-KRAB

    Plasmid

    #115547

    Purpose

    Entry cassette for dCas9-KRAB

    Depositor

    Rene Maehr

    Article

    Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

    Insert

    dCas9-KRAB
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LIC1B_Caspase-LOVN7C-7 C450A

    Plasmid

    #104628

    Purpose

    bacterial expression of light-activated caspase-3 with N7C-7 linker variation on C450A mutation

    Depositor

    Jim Wells

    Article

    Smart et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    Caspase-3 (CASP3 Human)
    

    Use

    Tags

    His and LOV2

    Expression

    Bacterial

    Mutation

    C450A (dark-locked and light-insensitive mutation)

    Promoter

    T7

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge