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171,784 results

Showing: 5101 - 5150 of 171784 results

  1. pODN-AAVS1-mR2-CAAX

    Plasmid
    #183871
    Purpose
    Repair template for the stable expression of a mRuby2-CAAX fusion in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    AAVS1 homology arms with CMV-driven mRuby2-CAAX fusion (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    mRuby2-CAAX
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    CMV
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183871-plasmid-map-sequence-id-373475

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  7. pD649-HAsp-ULBP1-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156597
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    ULBP1 (ULBP1 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156597-plasmid-map-sequence-id-420967

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  18. pHA-N1-GalT

    Plasmid
    #87326
    Purpose
    To express GalT-HA in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase
    Depositor
    Lei Lu
    Article
    Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15
    Insert
    1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV-F
    Available Since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87326-plasmid-map-sequence-id-177295

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  19. pAAV2/7

    Plasmid
    #112863
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap7
    Use
    AAV
    Promoter
    Truncated P5
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    112863-plasmid-map-sequence-id-364707

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  20. R300TA

    Plasmid
    #80411
    Purpose
    human cTNT minigene with genomic segment containing alternative exon 5 with CELF1 binding site mutated (CTGs->CAGs)
    Depositor
    Thomas Cooper
    Article
    Philips et al Science. 1998 May 1;280(5364):737-41.
    Insert
    TNNT2 (TNNT2 Human)
    Expression
    Mammalian
    Mutation
    CTGs downstream of alternative exon mutated to CA…
    Available Since
    Aug. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    80411-plasmid-map-sequence-id-151393

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  22. pCGFP-EU

    Plasmid
    #161968
    Purpose
    Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).
    Depositor
    Eric Gouaux
    Article
    Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1
    Type
    Empty backbone
    Tags
    EGFP His8
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    161968-plasmid-map-sequence-id-319729

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  27. pN1_Rev-GR-EGFP

    Plasmid
    #252750
    Purpose
    Plasmid for studying nuclear export. Expression of a fusion protein containing full-length HIV-1 Rev, followed by the hormone-responsive element of the rat glucocorticoid receptor (Gr), and GFP.
    Depositor
    Kristen Verhey
    Article
    Takao et al Curr Biol. 2014 Oct 6;24(19):2288-94. do
    Insert
    HIV-1 Rev and Glucocorticoid Receptor (GR)
    Tags
    EGFP
    Expression
    Mammalian
    Available Since
    March 19, 2026
    Availability
    Industry, Academic Institutions, and Nonprofits
    252750-plasmid-map-sequence-id-503503

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  30. PSB-CMV-Puro ArfGAP1_ALPS1_ALPS2-mKate2-3NLS-P2A-EGFP-Sec61B

    Plasmid
    #250913
    Purpose
    Dual color expression of mKate2-3NLS tagged human ARFGAP1 ALPS1-2 and EGFP tagged human Sec 61B in mammalian cell lines
    Depositor
    Philipp Niethammer
    Article
    Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/
    Insert
    ArfGAP1-mKate2-3xNLS-P2A-EGFP-Sec61B (SEC61B, ARFGAP1 Human)
    Tags
    EGFP on Sec61b and mKate2-3xNLS on ArfGAP1
    Expression
    Mammalian
    Mutation
    ARFGAP1 amino acids 192–304 only
    Promoter
    CMV
    Available Since
    Feb. 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    250913-plasmid-map-sequence-id-498650

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  32. PSB-CMV-Puro ArfGAP1_ALPS1-mkate2-3NLS-P2A-EGFP-Sec61B

    Plasmid
    #250912
    Purpose
    Dual color expression of mKate2-3NLS tagged rat ARFGAP1 ALPS1 and EGFP tagged human Sec 61B in mammalian cell lines
    Depositor
    Philipp Niethammer
    Article
    Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/
    Insert
    ArfGAP1-mKate2-3xNLS-P2A-EGFP-Sec61B (Arfgap1, SEC61B Human, Rat)
    Tags
    EGFP on Sec61B and mKate2-3xNLS on ArfGAP1
    Expression
    Mammalian
    Mutation
    ARFGAP1 amino acids 196–234 only
    Promoter
    CMV
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    250912-plasmid-map-sequence-id-498488

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  33. enDelIscB-T5E

    Plasmid
    #247330
    Purpose
    Vector encoding human codon-optimized engineered DelIscB fused with T5E at C-terminal driven by CAG promoter, optimized sgRNA (sgRNA-V5) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yong Tian
    Insert
    pCAG_enDelIscB-T5E_npNLS_polyA_pU6_sgRNA_V5-BsaI_pCMV_mCherry
    Expression
    Mammalian
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    247330-plasmid-map-sequence-id-496504

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  34. enDelIscB

    Plasmid
    #247329
    Purpose
    Vector encoding human codon-optimized engineered DelIscB driven by CAG promoter, optimized sgRNA (sgRNA-V5) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yong Tian
    Insert
    pCAG_enDelIscB_npNLS_polyA_pU6_sgRNA_V5-BsaI_pCMV_mCherry
    Expression
    Mammalian
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    247329-plasmid-map-sequence-id-496503

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  36. EPOR-LgBiT/EPOR-SmBiT Bidirectional Vector

    Plasmid
    #238776
    Purpose
    Express EPOR-LgBiT/EPOR-SmBiT Fusion Proteins in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Inserts
    EPOR (EPOR Human)
    EPOR (EPOR Human)
    Tags
    LgBiT and SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238776-plasmid-map-sequence-id-479908

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  38. 8061-E26

    Plasmid
    #234263
    Purpose
    Gateway ORF Entry clone of mouse Tgfb2 with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    Tgfb2 (Tgfb2 Mouse)
    Use
    Gateway entry clone
    Available Since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    234263-plasmid-map-sequence-id-468873

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  42. TFORF3307

    Plasmid
    #144783
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF563 (ZNF563 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144783-plasmid-map-sequence-id-404367

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  43. TFORF3096

    Plasmid
    #144572
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF837 (ZNF837 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144572-plasmid-map-sequence-id-404274

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  44. TFORF2754

    Plasmid
    #144230
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF446 (ZNF446 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144230-plasmid-map-sequence-id-404110

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  47. TFORF1368

    Plasmid
    #143793
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RREB1 (RREB1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143793-plasmid-map-sequence-id-396234

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  48. TFORF1642

    Plasmid
    #141858
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF426 (ZNF426 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141858-plasmid-map-sequence-id-415044

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  49. FGFR2 N549K 3xFlag

    Plasmid
    #110107
    Purpose
    expresses FGFR2 with a single amino acid change in mammalian cells
    Depositor
    Martin Fernandez-Zapico
    Insert
    fibroblast growth factor receptor 2 (FGFR2 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    mutated Asparagine 549 to Lysine (N549K)
    Promoter
    CMV
    Available Since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    110107-plasmid-map-sequence-id-209777

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Showing: 5101 - 5150 of 171784 results