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pCMV-Tag2B-NCreERT2

Plasmid

#106370

Purpose

N-terminal part of the split-CreERT2 System

Depositor

Johannes Hirrlinger

Article

Hirrlinger et al PLoS One. 2009 Dec 16;4(12):e8354. doi:

Insert

NCreERT2

Use

Cre/Lox

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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Myc-CICf

Plasmid

#48185

Purpose

Expresses Cic

Depositor

Huda Zoghbi

Article

Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

Insert

Capicua (Cic Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pET14b – lambda exonuclease

Plasmid

#104531

Purpose

bacterial expression of lambda exonuclease

Depositor

Charles Bell

Article

Zhang et al Proc Natl Acad Sci U S A. 2011 Jul 19;10

Insert

λ exonuclease

Tags

His6 followed by a thrombin site

Expression

Bacterial

Promoter

T7

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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myc-Atg14

Plasmid

#86747

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

human Atg14 (ATG14 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pMBP-HIS2-Snf7

Plasmid

#21492

Depositor

James Hurley

Article

Wollert et al Nature. 2009 Mar 12. 458(7235):172-7.

Insert

SNF7 (SNF7 Budding Yeast)

Tags

6x His and MBP

Expression

Bacterial

Mutation

none

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only

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TBX5-FLAG in pLX303

Plasmid

#42563

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

TBX5 (TBX5 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pSUPER IKKalpha

Plasmid

#26208

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

pSUPER IKK alpha (CHUK Human)

Use

RNAi

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pSUPER IKKbeta

Plasmid

#26209

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

pSUPER IKK beta (IKBKB Human)

Use

RNAi

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
shCTNNB1 # 1

Plasmid

#42543

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

CTNNB1 (CTNNB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP

Plasmid

#207863

Purpose

AAV genome encoding C-terminal PE6d and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP

Use

AAV

Mutation

See Manuscript

Promoter

Cbh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pBT114

Plasmid

#138523

Purpose

phage cloning

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

phage genes

Use

Synthetic Biology

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pKK-mClover3-TEV

Plasmid

#105778

Purpose

Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

mClover3-TEV

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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p2Bac.XPC

Plasmid

#39204

Depositor

Aziz Sancar

Article

Reardon et al J Biol Chem. 1996 Aug 9;271(32):19451-6.

Insert

XPC (XPC Human)

Expression

Insect

Mutation

Q475K polymorphism

Promoter

Pp10

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
pBiFCt-2in1-NN

Plasmid

#105111

Purpose

ratiometric BiFC analysis in plants (transient transformation)

Depositor

Christopher Grefen

Article

Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

Type

Empty backbone

Tags

nYFP/cYFP

Expression

Plant

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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3xmScarletI_UtrCH

Plasmid

#112962

Purpose

In vivo visualization of F-actin (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

UtrCH

Tags

3x mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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ZIKV_Sensor_27B_LacZ

Plasmid

#75006

Purpose

Toehold switch sensor to detect Zika Virus

Depositor

James Collins, Alexander Green

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_sensor_27B_LacZ

Tags

LacZ

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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shLacZ

Plasmid

#42559

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shLacZ

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
CMV-dCas13X.1-REPAIRv2-SV40pA_CMV-mCherry-BGHpA_U6-BbsI-DR

Plasmid

#171382

Purpose

Expression vector for encoding a human codon-optimized dCas13X.1-REPAIRv2 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

Depositor

Hui Yang

Article

Xu et al Nat Methods. 2021 May;18(5):499-506. doi

Insert

humanized dCas13X.1-REPAIRv2

Tags

HA

Expression

Mammalian

Promoter

CMV, U6

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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RP1B FUS 1-163

Plasmid

#127192

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Article

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 (FUS Human)

Tags

hexahistidine

Expression

Bacterial

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Utr230-EGFP

Plasmid

#58472

Purpose

Expresses a cytoplasmic actin filament reporter, a truncation of the tandem CH domains (a.a. 1-230) from human utrophin, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Bioarchitecture. 2015 Aug 28:1-14.

Insert

Utr230 (UTRN Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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