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pBabe_puro_DEST_Flag_PRKCI

Plasmid

#45263

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

PKCI (PRKCI Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

June 14, 2013

Availability

Academic Institutions and Nonprofits only
ama1CD4d3+4-blac

Plasmid

#36146

Purpose

EXPRESs plasmid for P. falciuparum merozoite surface proteins encoding P. falciparum ama1 with rat CD4d3+4-blac

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

ama1 (PF3D7_1133400 Plasmodium falciparum)

Tags

beta-lactamase, pentamerization domain of rat car…

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2012

Availability

Academic Institutions and Nonprofits only

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pEY61

Plasmid

#191058

Purpose

acr-2(3.7k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

acr-2(3.7k) (acr-2 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX SH2A-SH2

Plasmid

#46481

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SH2A (SH2D4A Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 338-445

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shStk16-1

Plasmid

#180391

Purpose

Producing AAV that encodes mouse Stk16 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shStk16-1 (Stk16 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-T2A-miniSOG-C-Far

Plasmid

#193915

Purpose

Expresses miniSOG targeted to the plasma membrane of neurons, joined by a T2A cleavage site with mCherry; for AAV production

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

miniSOG

Use

AAV

Tags

Farnesylation signal sequence and Flag tag

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX Slap2-SH2

Plasmid

#46501

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Slap2 (SLA2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 84-197

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Lnk-SH2

Plasmid

#46451

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Lnk (Sh2b3 Mouse)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 325-466

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pNAS1b split mCherry NEDD4-2 WW2

Plasmid

#111883

Purpose

Mode #2 phosphosite expression (split mCherry, using NEDD4-2 WW2 binding domain)

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Type

Empty backbone

Tags

Split mCherry system: NEDD4-2 WW2 fused to C-term…

Expression

Bacterial

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCDB181

Plasmid

#80692

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEHEE_06

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEHEE_06

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.mTagBFP2.let-7c

Plasmid

#169310

Purpose

Constitutive overexpression of let-7c with mTagBFP2 as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

let-7c (MIRLET7C Human)

Use

Lentiviral

Tags

TagBFP2

Promoter

SFFV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.dTomato.miR-125b-2

Plasmid

#169302

Purpose

Constitutive overexpression of miR-125b-2 with dTomato as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-125b-2 (MIR125B2 Human)

Use

Lentiviral

Tags

dTomato

Promoter

SFFV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX p85b(NC)-SH2

Plasmid

#46468

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

p85b(NC) (PIK3R2 Bovine)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 311-717

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pSECB sgMTHFD2L_1

Plasmid

#106306

Purpose

Express Cas9 and sgRNA targeting MTHFD2L

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTHFD2L (MTHFD2L Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSECB sgMTHFD2L_2

Plasmid

#106307

Purpose

Express Cas9 and sgRNA targeting MTHFD2L

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTHFD2L (MTHFD2L Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX Nck2-SH2

Plasmid

#46458

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

NM_001004720.2 (NCK2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 274-380

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Tem6-SH2

Plasmid

#46523

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Tem6 (TNS3 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1171-1291

Promoter

Tac

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pPHR-EYFP

Plasmid

#103817

Purpose

Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP (CRY2 Mustard Weed)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Vrap-SH2

Plasmid

#46531

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Vrap (SH2D2A Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 93-192

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe_puro_DEST_Flag_TLOC1 (220aa)

Plasmid

#45273

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

TLOC1 (SEC62 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pCas9GG

Plasmid

#131009

Purpose

Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Inserts

direct repeat of SpCas9
promoter PJ23119
mRFP expression cassette

Use

CRISPR

Expression

Bacterial

Promoter

Bba_R0040 TetR

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX BRDG1-SH2

Plasmid

#46410

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

BRDG1 (STAP1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 167-283

Promoter

Tac

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 HRPT2 L64P

Plasmid

#11052

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

HRPT2 L64P (CDC73 Human)

Tags

Flag

Expression

Mammalian

Mutation

L64P

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
pGEX Shb-SH2

Plasmid

#46485

Depositor

Bruce Mayer, Michael Welsh

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Shb (SHB Human)

Tags

GST, HA, and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 401-508

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 Myc Rheb Omp25

Plasmid

#112764

Purpose

express myc-Rheb-Omp25

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

myc-Rheb-Omp25 (RHEB Human)

Tags

Omp25 and myc

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-FKBP-TPD52

Plasmid

#172455

Purpose

Expression of Tumor Protein D52 (TPD52) with N-terminal GFP-FKBP tag

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD52 (TPD52 Human)

Tags

GFP-FKBP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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GST-TPD54

Plasmid

#172462

Purpose

Bacterial expression of GST-tagged Tumor Protein D52 like 2 (TPD54/TPD52L2)

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD54 (TPD52L2 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Vav3-SH2

Plasmid

#46530

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Vav3 (Vav3 Mouse)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 660-774

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
SIN40C.SFFV.GFP.miR-99a

Plasmid

#169304

Purpose

Constitutive overexpression of miR-99a with GFP as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-99a (MIR99A Human)

Use

Lentiviral

Tags

EGFP

Promoter

SFFV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Arp3_pLib

Plasmid

#173677

Purpose

Arp3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

Arp3 (ACTR3 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-FKBP-TPD52

Plasmid

#172456

Purpose

Expression of Tumor Protein D52 (TPD52) with N-terminal mCherry-FKBP tag

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD52 (TPD52 Human)

Tags

mCherry-FKBP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Tenc1-SH2

Plasmid

#46524

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Tenc1 (TNS2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1114-1264

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES-EGFP-TPD52

Plasmid

#172460

Purpose

Expression of untagged Tumor Protein D52 (TPD52) and GFP

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD52 (TPD52 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Socs5-SH2

Plasmid

#46508

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Socs5 (SOCS5 Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 378-481

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pX458-sgRNA_Ago1_3

Plasmid

#73535

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO1

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
pGEX Grap-SH2

Plasmid

#46434

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grap (GRAP Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 51-160

Promoter

Tac

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pET51b-His-TEV-SNAP-tag_cx

Plasmid

#167273

Purpose

Overexpression of crystallizing SNAP-tag variant (P179R) in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAP-tag

Tags

His x10

Expression

Bacterial

Mutation

P179R

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Cten-SH2

Plasmid

#46421

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Cten (TNS4 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 444-570

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW1997_pExpr-hU6-CRISPR-DECODER

Plasmid

#87549

Purpose

Expresses NGN2, MIAT, ACTC1, and TTN gRNAs in response to combinations of Cre and Flp recombinases

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

gRNAs targeting NGN2, MIAT, ACTC1, TTN

Use

CRISPR, Cre/Lox, and Synthetic Biology

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-TetO-Sox15

Plasmid

#128831

Purpose

dox-dependent expression of mouse Sox15

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Sox15 (Sox15 Mouse)

Use

Lentiviral

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 4121 - 4160 of 7580 results

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We narrowed to 7,580 results for: Ski;