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Showing: 5151 - 5200 of 16065 results

  7. pSBbi_RP_3XNLS-MicroID2-FLAG

    Plasmid
    #188523
    Purpose
    Encodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cells
    Depositor
    James Londino
    Article
    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.
    Insert
    3XNLS-MicroID2
    Use
    Transposon mediated gene integration
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188523-plasmid-map-sequence-id-372834

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  12. ePPEmax*

    Plasmid
    #205244
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Zong Yuan
    Article
    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:
    Insert
    c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Detailed in manuscript
    Promoter
    Available since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    205244-plasmid-map-sequence-id-422881

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  23. pL-SFFV.Reporter.RFP657.PAC

    Plasmid
    #61395
    Purpose
    Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.
    Depositor
    Benjamin Ebert
    Article
    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    61395-plasmid-map-sequence-id-101662

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  29. pJRH056

    Plasmid
    #171637
    Purpose
    Plasmid containing a U6 promoter expressing a spyCas9 tdTom sgRNA298 and CAG promoter expressing mTagBFP2
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-tdTomato
    mTagBFP2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171637-plasmid-map-sequence-id-354439

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  33. pFLAG_attP

    Plasmid
    #110095
    Purpose
    Vector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110095-plasmid-map-sequence-id-317064

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Showing: 5151 - 5200 of 16065 results