162,717 results

1. PB-U6

   Plasmid

   #120358

   Purpose

   piggyBac transposon vector for sgRNA expression.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBTBX-1

   Plasmid

   #26067

   Depositor

   Ryan Gill

   Article

   Prior et al Biotechnol Bioeng. 2010 Jun 1. 106(2):32

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pK.myc-MYPT1

   Plasmid

   #24101

   Depositor

   Masumi Eto

   Article

   Eto et al Cell Motil Cytoskeleton. 2005 Oct . 62(2

   Insert

   Myosin-targeting subunit-1 (PPP1R12A Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   S50G, P415Q

   Promoter

   Available since

   March 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. 763 pME mApple

   Plasmid

   #195960

   Purpose

   Gateway pME fluorophore

   Depositor

   Kristen Kwan, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   mApple
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF2004

   Plasmid

   #143330

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOXD1 (FOXD1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP(C1)-PP1alpha

   Plasmid

   #44224

   Depositor

   Laura Trinkle-Mulcahy, Angus Lamond

   Article

   Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

   Insert

   PP1A (PPP1CA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. p52 cFlag pcDNA3

   Plasmid

   #20019

   Depositor

   Stephen Smale

   Insert

   Nfkb2 (Nfkb2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Truncated at the p100 ligation site. It codes for…

   Promoter

   Available since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

8. nSYB-GAL80-DD

   Plasmid

   #124778

   Purpose

   Expresses GAL80 fused with destabilizing domain (DD) downstream of a n-Synaptobrevin promoter

   Depositor

   Jing-W Wang

   Article

   Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

   Insert

   TMP-inducible destabilized GAL80 (GAL80-DD)
   

   Use

   Tags

   DD

   Expression

   Insect

   Mutation

   Promoter

   nSynaptobrevin

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

   Plasmid

   #139093

   Purpose

   Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   dxCas9-NG-VPR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWB511

    Plasmid

    #74853

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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