We narrowed to 2,116 results for: PORA

1. pRCA895 - pBA904 Puro-T2A-BFP KLF4 g1 CRISPRa guide guide (pRCA594 backbone) 668

   Plasmid

   #238187

   Purpose

   Lentiviral CRISPR guide vector expressing a KLF4 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

   Depositor

   Thomas Norman

   Article

   Southard et al Nat Genet (2025)

   Insert

   KLF4 sgRNA CRISPRa (KLF4 Synthetic, Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   mTagBFP2

   Expression

   Mutation

   Promoter

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGhMeCP2 (pc3972)

   Plasmid

   #212033

   Purpose

   Expresses human MeCP2 tagged N-terminal to Halo-tag

   Depositor

   Cristina Cardoso

   Article

   Zhang et al Nucleus. 2022 Dec;13(1):1-34. doi: 10.10

   Insert

   MeCP2 (MECP2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-6xTRE-FLEX-hM4Di.mCherry

   Plasmid

   #169415

   Purpose

   Activity-dependent, cre-dependent, temporally restricted inhibitory DREADD expression

   Depositor

   David Talmage

   Article

   Rajebhosale et al Elife. 2024 Feb 16;13:e86581. doi: 10.75

   Insert

   hM4Di.mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   hM4Di is Gi-coupled DREADD.Derived from human mus…

   Promoter

   Minimal TATA

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDule2-3-nitroTyrosine (A7)

   Plasmid

   #174079

   Purpose

   Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470

   Insert

   3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y32H H70T D158H I159A L162R

   Promoter

   GlnRS (constitutive)

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDule-3-nitroTyrosine (A7)

   Plasmid

   #174078

   Purpose

   Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470

   Insert

   3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y32H H70T D158H I159A L162R

   Promoter

   GlnRS (constitutive)

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCJ202

   Plasmid

   #162675

   Purpose

   pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   W159C, S238C; codon optimized for expression in E…

   Promoter

   T7/lac

   Available since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCJ197

   Plasmid

   #162670

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S131G mutation.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   S131G; codon optimized for expression in E. coli …

   Promoter

   T7/lac

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LgBiT-KRAS(G12V)_SmBiT-CRAF-RBD Bidirectional Vector

   Plasmid

   #237067

   Purpose

   Express LgBiT-KRAS(G12V)_SmBiT-CRAF-RBD Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Inserts

   KRAS (KRAS Human)
   cRAF(RBD) (RAF1 Human)
   

   Use

   Tags

   LgBiT and SmBiT

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. LgBiT-KRAS (WT)_SmBiT-CRAF-RBD Bidirectional Vector

   Plasmid

   #237066

   Purpose

   Express LgBiT-KRAS (WT)_SmBiT-CRAF-RBD Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Inserts

   KRAS (KRAS Human)
   cRAF(RBD) (RAF1 Human)
   

   Use

   Tags

   LgBiT and SmBiT

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. cRAF CRD-RBD: KRAS G12C BiBRET Vector

    Plasmid

    #236872

    Purpose

    Express cRAF CRD-RBD: KRAS G12C in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    cRAF(RBD)-CRD (RAF1 Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    G12C

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. Nluc-KRAS(G12D)-cRAF(RBD)-HaloTag BiBRET Vector

    Plasmid

    #236867

    Purpose

    Express NanoLuc(R)-KRAS(G12D)-cRAF(RBD)-HaloTag(R) in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    cRAF(RBD) (RAF1 Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    G12D

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. Nluc-KRAS(G12C)-cRAF(FL)-HaloTag BiBRET Vector

    Plasmid

    #236868

    Purpose

    Express NanoLuc(R)-KRAS(G12C)-cRAF(FL)-HaloTag(R) in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    cRAF (RAF1 Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    G12C

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. Nluc-KRAS(G12C)-cRAF(RBD)-HaloTag BiBRET Vector

    Plasmid

    #236869

    Purpose

    Express NanoLuc(R)-KRAS(G12C)-cRAF(RBD)-HaloTag(R) in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    cRAF(RBD) (RAF1 Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    G12C

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. NanoBRET BiBRET Vector HaloTag-KRAS 2B WT-BRAF RBD-NanoLuc

    Plasmid

    #236853

    Purpose

    Express HaloTag(R)-KRAS 2B WT-BRAF RBD-NanoLuc(R) in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    BRAF (RBD) (BRAF Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pAAV-TNT4-TadA8e-SpRY N aa2-713-InteinN-miR122 TS

    Plasmid

    #209787

    Purpose

    Expresses TadA8e and SpRY cas9N by the specific TNT4 promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)

    Depositor

    Yuxuan Guo

    Article

    Yang et al Circulation. 2024 May 28;149(22):1778-17

    Insert

    TNT4, TadA8e, SpRY N, inteinN, miR122 TS
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TNT4

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCJ205

    Plasmid

    #162676

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224A an

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C224A, C529A; codon optimized for expression in E…

    Promoter

    T7/lac

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCJ198

    Plasmid

    #162671

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating F495I mutation.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    F495I; codon optimized for expression in E. coli …

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCJ196

    Plasmid

    #162669

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating catalytic mutation, S225A

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    S225A; codon optimized for expression in E. coli …

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCJ204

    Plasmid

    #162677

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224H and C529F mutations.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C224H, C529F; codon optimized for expression in E…

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCJ201

    Plasmid

    #162674

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224W and C529SS mutations.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C224W, C529SS; codon optimized for expression in …

    Promoter

    T7/lac

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge