59,458 results

1. VPS13A^mCherry

   Plasmid

   #118758

   Purpose

   VPS13A internally tagged with mCherry at residue 1372.

   Depositor

   Pietro De Camilli

   Article

   Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

   Insert

   VPS13A (VPS13A Human)
   

   Tags

   mCherry inserted at position 1372

   Expression

   Mammalian

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221_MFSD8

   Plasmid

   #131882

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   MFSD8 (MFSD8 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 9, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pLenti-MetGFP

   Plasmid

   #37560

   Depositor

   David Rimm

   Insert

   MET (MET Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-HSF1

   Plasmid

   #32537

   Depositor

   Stuart Calderwood

   Article

   Wang et al Mol Cell Biol. 2003 Sep;23(17):6013-26.

   Insert

   HSF1 (HSF1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   see depositor comments below

   Promoter

   CMV

   Available Since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCTRE-CD4

   Plasmid

   #114010

   Purpose

   Dox-inducible Cas9 expression with a CD4 selection marker

   Depositor

   Charles Rudin

   Article

   Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-N1 Dynamin1 wt

   Plasmid

   #120313

   Purpose

   Mammalian expression plasmid of GFP-tagged Dynamin protein.

   Depositor

   Justin Taraska

   Article

   Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

   Insert

   Dynamin1 (DNM1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLQ1852-2 pHR: U6-SpsgCD95-1 CMV-EGFP

   Plasmid

   #84251

   Purpose

   Expresses Sp sgCD95-1 gRNA with an EGFP fluorescent marker

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Insert

   Sp sgCD95-1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF0731

   Plasmid

   #142201

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PROX1 (PROX1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-HIF1alpha-wt-pBabe-puro

   Plasmid

   #19365

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 1 alpha (HIF1A Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wt human cDNA

   Available Since

   April 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. FH-TET3-pEF

    Plasmid

    #49446

    Purpose

    This plasmid expresses full-length human TET3.

    Depositor

    Anjana Rao

    Article

    Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

    Insert

    Tet methylcytosine dioxygenase 3 (TET3 Human)
    

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    EF-1alpha

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. tFucci(SA)5

    Plasmid

    #153520

    Purpose

    New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

    Insert

    tFucci(SA)5.
    

    Tags

    AzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)

    Expression

    Bacterial and Mammalian

    Promoter

    CAG

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-NORAD

    Plasmid

    #120383

    Purpose

    Expression vector for lncRNA NORAD

    Depositor

    Igor Ulitsky

    Article

    Tichon et al Nat Commun. 2016 Jul 13;7:12209. doi: 10

    Insert

    NORAD (NORAD Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLNHA-C1-HsXRN1

    Plasmid

    #66596

    Purpose

    expresses lambdaN-HA-tagged XRN1 in mammalian cells

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

    Insert

    XRN1 (XRN1 Human)
    

    Tags

    lambdaN-HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. hE-cadherin-pcDNA3

    Plasmid

    #45769

    Depositor

    Barry Gumbiner

    Article

    Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

    Insert

    hE-cadherin (CDH1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMMP-MCS-IRES-eGFP-P2A-iCas9

    Plasmid

    #178533

    Purpose

    For genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cells

    Depositor

    Ryoji Yao

    Article

    Okamoto et al STAR Protoc. 2021 Sep 16;2(4):100780. do

    Insert

    iCaspase9 (CASP9 Human)
    

    Expression

    Mammalian

    Mutation

    FKBP12 F36 to V Truncated Caspase9 (135-416; lack…

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. anxA2-GFP

    Plasmid

    #107196

    Purpose

    Expresses human annexin A2-GFP fusion protein for live cell imaging

    Depositor

    Volker Gerke, Ursula Rescher

    Article

    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

    Insert

    annexin A2 (ANXA2 Human)
    

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    Ala residue 65 in human sequence replaced by Glu …

    Promoter

    CMV

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pD649-HAsp-DLL4-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156889

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    DLL4 (DLL4 Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. p23-puro-EGFP-RPA194

    Plasmid

    #179237

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    RPA194 (POLR1A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF-1alpha

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. MAC_N_FEV

    Plasmid

    #167757

    Purpose

    Expresses FEV in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    FEV (FEV Human)
    

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5-mEGFP-SQSTM1-WT

    Plasmid

    #194582

    Purpose

    Mammalian Expression of mEGFP-SQSTM1 WT Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    SQSTM1 (SQSTM1 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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