We narrowed to 7,778 results for: CCH
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Plasmid#127756PurposeResearch the effect of gene orientation of transcriptionDepositorInsertmSpinach,RFP Divergent Orientation
UseSynthetic BiologyExpressionBacterialAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AktAR2
Plasmid#64932PurposeEnhanced FRET-based biosensor for monitoring Akt activity.DepositorInsertAktAR2
TagsCerulean3 and cpVenus[E172]ExpressionMammalianPromoterCMVAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFGET19_Ulp1
Plasmid#64697PurposeH6-Ulp1 from pHYRS52 into pET-28b(+)DepositorInsertULP1 (ULP1 Saccharomyces cerevisiae, Budding Yeast)
Tags6xHisExpressionBacterialMutationUlp1(403-621)PromoterT7Available SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL2B -1538/+64
Plasmid#24942DepositorAvailable SinceAug. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
Gal4-VP16
Plasmid#71728Purposeplasmid expressing fusion construct of Gal4 DBD and VP16 ADDepositorAvailable SinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET29b-IPP1-His
Plasmid#124137PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM236 Ubc4
Plasmid#226361PurposeExpression of yeast Ubc4DepositorAvailable SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM102
Plasmid#226694Purpose6xHis-Sumo-Ub-Ub-Ub-UbDepositorAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS313-SCT1-S288C
Plasmid#226266PurposePlasmid expressing the SCT1 allele from S288C, under control of its native promoterDepositorInsertSCT1 (SCT1 Budding Yeast)
ExpressionYeastAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
FRP793_insul-(lexA-box)4-PminCYC1-Citrine-TCYC1
Plasmid#58434PurposeInduces Citrine expression from a promoter containing 4 lexA boxes in yeast cellsDepositorInsertCitrineA206K
ExpressionYeastPromoterinsul-(lexA-box)4-PminCYC1Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pTRKH3-ermGFP
Plasmid#27169DepositorInserterythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5
ExpressionBacterialMutationpresence of an additional 183-bp region at N term…Available SinceFeb. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLOX-Ttag-iresTK
Plasmid#12246DepositorInsertSV40 Large T, HSV1-TK (SV40gp6 Simian virus)
UseCre/Lox and LentiviralExpressionMammalianMutationLoxP in 3' LTR.Available SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6
Plasmid#39296DepositorInsertKanR
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…Available SinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJL1 ATF1
Plasmid#106286PurposeExpresses ATF1 in Ecoli off a T7 promoterDepositorInsertATF1
ExpressionBacterialPromoterT7Available SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
LT1_34_sfpHluorin
Plasmid#177707PurposePlasmid for genome integration in X2 site expressing sfpHluorin (intracellular pH biosensor)DepositorInsertpTEF1-sfpHluorin
UseUsed as donor in genome integration after lineari…ExpressionYeastAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-Cerulean-YPT7
Plasmid#18847DepositorAvailable SinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDB5320
Plasmid#204830PurposeAn integrating plasmid containing both the inducible enotetSW2 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW2 promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDB5318
Plasmid#204828PurposeAn integrating plasmid containing both the inducible enotetS promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetS promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDUP3
Plasmid#92174PurposeVector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by duplication.DepositorInsertNAT1
UseGenbank: u03440.1ExpressionYeastAvailable SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTPGI_dCas9_VP64
Plasmid#49013Purposeencodes yeast-optimized dCas9_VP64 synthetic transcription factorDepositorInsertdCas9_VP64 (codon-optimized for expression in S. cerevisiae)
UseCRISPR and Synthetic BiologyTagsSV40 NLSExpressionYeastPromoterpTPGI (galactose+aTc inducible)Available SinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a URA3
Plasmid#61924PurposeExpression of S. cerevisiae URA3 cloned into pFA6aDepositorInsertURA3
UseYeast genomic targetingAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDB5319
Plasmid#204829PurposeAn integrating plasmid containing both the inducible enotetSW1 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW1 promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-flpE-puro
Plasmid#20733PurposeMammalian expression of FLPe recombinase from the highly-expressed CAGGS promoter; puro selectable markerDepositorInsertFLPe recombinase
UseFlp/frtExpressionMammalianPromoterCAGGSAvailable SinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSems LifeAct-StayGold
Plasmid#222947PurposeExpression of LifeAct tagged with StayGold in mammalian cellsDepositorInsertLifeAct
TagsStayGoldExpressionMammalianPromoterCMVAvailable SinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-IPP1-His
Plasmid#118978PurposeBacterial expression plasmid for Inorganic pyrophosphataseDepositorInsertinorganic diphosphatase IPP1 (IPP1 Budding Yeast)
Tags6x-HisExpressionBacterialPromoterT7 promoterAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-His3MX6
Plasmid#41596DepositorInsertHis3
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…Available SinceMay 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 Stu2 1-590
Plasmid#38314DepositorAvailable SinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::LEU2
Plasmid#227594PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::LEU2
Tags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIM701
Plasmid#74642Purposealias pFA6a-ymTagBFP-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and nourseothricin selection markerDepositorInsertymTagBFP
ExpressionYeastPromoternoneAvailable SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
mito-mScarlet
Plasmid#141153PurposeFluorescent labeling of the mitochondrial matrix in mammalian cellsDepositorAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDest17_FL Gcn4
Plasmid#231855PurposeBacterial expression of N-terminally 6His tagged FL Gcn4DepositorInsertGcn4
Tags6xHis, TEV cleavage siteExpressionBacterialPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-AKAR4-Kras
Plasmid#61621PurposeNon-raft/plasma membrane-targeted FRET biosensor for monitoring PKA activityDepositorInsertAKAR4-Kras
TagsC-terminal targeting sequence from Kras, Cerulean…ExpressionMammalianPromoterCMVAvailable SinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYZ463
Plasmid#187971PurposeTEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4tDepositorInsertCas9 (codon optimized for human)
UseSynthetic BiologyTagsSV40ExpressionYeastPromoterTEF1Available SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-AKAR4
Plasmid#61620PurposeLipid raft-targeted FRET biosensor for monitoring PKA activityDepositorInsertLyn-AKAR4
TagsCerulean, N-terminal targeting sequence from Lyn …ExpressionMammalianPromoterCMVAvailable SinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETM6-mCherry
Plasmid#66534PurposeExpresses mCherry under the control of the Concensus T7 PromoterDepositorInsertmCherry
UseSynthetic BiologyExpressionBacterialMutationCodon Optimized for E. coliPromoterConsensus T7Available SinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::URA3
Plasmid#227595PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::URA3
Tags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pYZ038
Plasmid#200227Purposeuniversal template to build tRNA-gRNA array for multiplex editing CREEPYDepositorInserttRNA-gRNA scaffold
UseSynthetic BiologyAvailable SinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only