We narrowed to 5,265 results for: 290

1. pBabe puro HA PIK3CA E545K

   Plasmid

   #12525

   Depositor

   Jean Zhao

   Article

   Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

   Insert

   PIK3CA E545K (PIK3CA Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E545K. Tumor-derived allele.

   Available Since

   Aug. 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiCRISPRv2 EGFP sgRNA1

   Plasmid

   #164932

   Purpose

   Expresses EGFP sgRNA1 in mammalian cells

   Depositor

   Ji Luo

   Article

   Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

   Insert

   sgRNA1 targeting Enhanced green fluorescent protein (verified for knockout)
   

   Use

   Lentiviral

   Promoter

   EFS promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK-caCas9-NatMX-Neut5L-Leu2 drive

   Plasmid

   #89578

   Purpose

   caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus

   Depositor

   George Church

   Article

   Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

   Insert

   Leu2 gene drive
   

   Expression

   Yeast

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe puro Myr HA PIK3CA

   Plasmid

   #12523

   Depositor

   Jean Zhao

   Article

   Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

   Insert

   PIK3CA Myr (PIK3CA Human)
   

   Use

   Retroviral

   Tags

   HA and Myr

   Expression

   Mammalian

   Mutation

   Myristoylation. Constitutively active allele; I14…

   Available Since

   Aug. 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. IRES-GFP FoxG1

   Plasmid

   #14935

   Depositor

   Joan Massague

   Article

   Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

   Insert

   FoxG1 (FOXG1 Human)
   

   Tags

   IRES-GFP

   Expression

   Mammalian

   Available Since

   May 17, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-hPGK-eGFP-SynaptoZip

   Plasmid

   #122523

   Purpose

   Lentiviral expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by hPGK promoter

   Depositor

   Antonio Malgaroli

   Article

   Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

   Insert

   eGFP-SynaptoZip (Vamp2 Rat)
   

   Use

   Lentiviral

   Tags

   Myc and eGFP

   Expression

   Mammalian

   Promoter

   hPGK

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFsynW SYP-GFP

   Plasmid

   #197283

   Purpose

   Encodes for viral expression of full-length wild type synaptophysin (SYP). Visualized by a C-terminal GFP.

   Depositor

   Edwin Chapman

   Article

   Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

   Insert

   SYP and GFP (Syp Rat)
   

   Use

   Lentiviral

   Tags

   1D4 epitope

   Mutation

   N/A

   Promoter

   hSyn (human synapsin I promoter)

   Available Since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p5xUAS-Flag-NPM1c

   Plasmid

   #131823

   Purpose

   4-hydroxytamoxifen inducible vector expressing Flag-NPM1c (cytoplasmic mutant, human) in mammalian cells through transduction

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   NPM1 (NPM1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPBbsr2-Raichu-454HRasCT

   Plasmid

   #176029

   Purpose

   A FRET biosensor for HRas activity

   Depositor

   Michiyuki Matsuda

   Article

   Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

   Insert

   Raichu-Ras-HRasCT
   

   Expression

   Mammalian

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. tUI

    Plasmid

    #122661

    Purpose

    Express tUI protein in E. coli

    Depositor

    Robert Cohen

    Article

    Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Insert

    tUI (UBQLN1, USP5 Human)
    

    Tags

    His-tag

    Promoter

    T7 promoter

    Available Since

    July 17, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. mouse CENP-B-EGFP

    Plasmid

    #107264

    Purpose

    mouse CENP-B-EGFP in pIVT vector for in vitro transcription

    Depositor

    Michael Lampson

    Article

    Akera et al Science. 2017 Nov 3;358(6363):668-672. d

    Insert

    CENP-B-EGFP (Cenpb Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV Syn CaMPARI2-human-a-Tubulin (TubuTag)

    Plasmid

    #164184

    Purpose

    Freeze-frame imaging of dendritic calcium signals CaMPARI2 moiety with lower Ca+ affinity (Kd=199nM) (Moeyaert et al., 2018)

    Depositor

    Thomas Oertner

    Article

    Perez-Alvarez et al Front Mol Neurosci. 2021 Mar 4;14:635820

    Inserts

    CamPARI2
    Human α Tubulin
    

    Use

    AAV

    Tags

    6 His tag and NES

    Expression

    Mammalian

    Promoter

    Syn

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBtPIPLC His

    Plasmid

    #173808

    Purpose

    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells

    Depositor

    Anne Gershenson, Mary F Roberts

    Article

    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4

    Insert

    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. hHSF1 K80Q

    Plasmid

    #118351

    Purpose

    Plasmid expresses hHSF1 with K80 in DBD mutated to Q, which abolish DNA-binding activity of HSF1.

    Depositor

    Lea Sistonen

    Article

    Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

    Insert

    hHSF1 (HSF1 Human)
    

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K80Q

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQCXIP EGFP-ER [TM(SAC1)]

    Plasmid

    #186475

    Purpose

    Expression of the Endoplasmic Reticulum marker GFP-ER [TM(SAC1)] in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    SAC1 (SACM1L Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C-terminal transmembrane region of the protein: 5…

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pInducer20 CARD8-HA PAM

    Plasmid

    #169982

    Purpose

    Tet/Dox-inducible mammalian expression of CARD8 with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PAM

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 6B CARD8 UPA-CARD-mCherry R464E

    Plasmid

    #164013

    Purpose

    Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    R464E

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-FKBP-SAC1-HLx2

    Plasmid

    #108133

    Purpose

    Recruitable SAC1 with extended linker

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:FKBP1A(3-108):[GGSA]4GG::SACM1L(1-520):[EAAAR]2:SACM1L(521-587) (SACM1L Human)
    

    Expression

    Mammalian

    Mutation

    SACM1L(1-520):[EAAAR]2:SACM1L(521-587)

    Promoter

    CMV

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. H52-pUCM-hNGN2-inv

    Plasmid

    #110492

    Purpose

    Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm

    Depositor

    Michael Ward

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    mcherry
    rtTA3G
    

    Use

    CRISPR, Cre/Lox, and TALEN

    Expression

    Mammalian

    Promoter

    CAG, EF-1 alpha, and TRE3G

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLN539 (FuGW-G8p-antiPD1(HC)-P2A-antiPD1(LC)-1Pe)

    Plasmid

    #105200

    Purpose

    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab transcripts containing miR1-Bs(Pe)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G8p-antiPD1(HC)-P2A-antiPD1(LC)
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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