We narrowed to 14,439 results for: eng
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Plasmid#58502PurposeExpresses the pH sensor ratiometric pHluorin in the mitochondria of mammalian cellsDepositorInsert1xCox8 - Ratiometric pHluorin
UseLentiviralExpressionMammalianPromotera-CamKIIAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p416 103Q GAL
Plasmid#1186DepositorAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HF-PX459 (V2)
Plasmid#118632PurposePlasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistanceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthSpCas9-HF1-2A-Puro V2.0
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationAsparagine 497 to Alanine, Arginine 661 to Alanin…PromoterCbhAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZ149-pcDNA3-BhCas12b_v4
Plasmid#122446PurposeExpresses BhCas12b_v4 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCMV-NLS-BhCas12b_v4-NLS-3xHA
ExpressionMammalianAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc
Plasmid#117728PurposeIntegration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc geneDepositorInsertNanoLuc
UseLuciferaseExpressionBacterialAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMOS001: CMV-Optopatch2_FCK
Plasmid#62984PurposeCoexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology under CMV promoterDepositorInsertOptopatch2
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV-CDH5-mRuby3-IRES-puro
Plasmid#85144Purposelentiviral expression of human VE-cadherin, C-terminally tagged with mRuby3DepositorInsertCDH5-mRuby3 (VE-cadherin, C-terminally tagged with mRuby3 (CDH5 Human)
UseLentiviralTagsmRuby3PromoterCAGAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
bNS2B47NS3
Plasmid#155320Purposeco-expression of DENV4 47 peptides from NS2B cofactor and NS3 full lengthDepositorInsertCo expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor
TagsHis tagExpressionBacterialMutationWTPromoterT7Available sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDe-CAS9
Plasmid#61433PurposeBinary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequenceDepositorInsertCas9
UseCRISPRExpressionPlantPromoterPcUBI4-2Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
scAAV-Cre
Plasmid#177933PurposeExpresses Cre-recombinase and can be packaged into AAV particles.DepositorInsertCAG-Cre
UseAAVExpressionMammalianPromoterU6Available sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-mNeonGreen
Plasmid#99131PurposeAn AAV genome with tet-inducible expression of the fluorescent protein mNeonGreenDepositorInsertmNeonGreen
UseAAVExpressionMammalianPromoterTREAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV-CASANOVA
Plasmid#113035PurposeCASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
UseCRISPR and Synthetic BiologyExpressionMammalianAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDR-GFPuniv
Plasmid#46085PurposeIn vivo recombination assay fluorescent reporterDepositorInsertDR-GFPuniv reporter
UseRecombination reporter plasmidExpressionMammalianMutationE5G and K163R mutations in EGFP relative to wild-…Available sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFH2.10_Gag-MCP-P2A-eGFP
Plasmid#205525PurposeExpress HIV Gag-MCP-P2A-eGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGag-MCP
ExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-PUM1-HD
Plasmid#17543DepositorPublicationAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSP1673
Plasmid#65769PurposeBacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNADepositorInsertmammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
UseCRISPRTagsNLSExpressionBacterialPromoterT7 (x2)Available sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA3.1(+)-NEMOc
Plasmid#189933PurposeMammalian expression of calcium sensor with ultra-high dynamics and sensitivityDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNEMOc
ExpressionMammalianPromoterCMVAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
1006 pSG5L Myr HA PTEN
Plasmid#10776DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPTEN (PTEN Human)
TagsHA and MyrExpressionMammalianAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.2 TDP-43 NLS1, 5F-L YFP
Plasmid#84913PurposeMammalian expresion of TDP-43 NLS1, 5F-L YFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTDP-43 (TARDBP Human)
TagsYFPExpressionMammalianMutationK82A, R83A, K84A, F147L, F149L, F194L, F229L, F23…PromoterCMVAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSEVA331Bb
Plasmid#78269PurposepSEVA331 with Biobrick multiple cloning siteDepositorTypeEmpty backboneAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by