4,076 results

1. pRK793

   Plasmid

   #8827

   Depositor

   David Waugh

   Article

   Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

   Insert

   TEV protease, S219V mutant
   

   Use

   Tags

   His, MBP (with TEV), and polyarginine

   Expression

   Bacterial

   Mutation

   S219V mutation (improves stability)

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-KRAS-G12D

   Plasmid

   #116423

   Purpose

   Lentiviral expression of KRAS G12D

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   KRAS (KRAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   G12D

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-Rab5a-7

   Plasmid

   #55126

   Purpose

   Localization: Ras GTPase, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Rab5a
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-tdPP7-3xmCherry

   Plasmid

   #74926

   Purpose

   Expresses tdPP7-3xmCherry

   Depositor

   Marvin Tanenbaum

   Article

   Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

   Insert

   PP7-3xmCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB_tre_dCas9_KRAB

   Plasmid

   #126030

   Purpose

   PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Inserts

   dCas9 fusion with KRAB
   Hygromycin resistance
   

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   EF1 alpha and TRE

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)

   Plasmid

   #101139

   Purpose

   Luciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET His6 LIC cloning vector (2Bc-T)

   Plasmid

   #37236

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. AbVec1.1-IGKC

   Plasmid

   #80796

   Purpose

   Expression of immunoglobulin light chains in mammalian cells, human kappa constant region

   Depositor

   Hedda Wardemann

   Article

   Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pet30b-7M SrtA

   Plasmid

   #51141

   Purpose

   expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity

   Depositor

   Hidde Ploegh

   Insert

   S.aureus SrtA 7M
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   P94R, E105K, E108Q, D160N, D165A, K190E, K196T

   Promoter

   T7

   Available since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-beta-catenin deltaN90

    Plasmid

    #36985

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    beta-catenin deltaN90 (CTNNB1 Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 1-90 (Constitutively Acti…

    Promoter

    EF1a

    Available since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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