We narrowed to 10,816 results for: HA

1. pGEM-T-Easy-HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT

   Plasmid

   #119065

   Purpose

   Donor construct to amplify HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT
   

   Use

   Tags

   HA

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. KK701: pMAGIC (L3-L2) 3x HA eptitope tag + polyA; hU6::xCas9(3.7) gRNA scaffold

   Plasmid

   #121842

   Purpose

   pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven xCas9(3.7) gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA fusion to dCas9 w/ gRNA expression

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLV-EF1A-WWE(R163A)-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro

   Plasmid

   #187612

   Purpose

   Split luciferase construct with nLuc fused to C-terminus of WWE domain with Arg163Ala mutation, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii

   Inserts

   WWE domain (R163A) with C-terminal nLuc
   WWE domain with C-terminal cLuc
   

   Use

   Lentiviral

   Tags

   FLAG, HA, Split luciferase (cLuc), Split lucifera…

   Expression

   Mammalian

   Mutation

   Arg163Ala

   Promoter

   EF1A

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. JV201: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#1 gRNA

   Plasmid

   #131755

   Purpose

   pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   TRE#1 SaCas9 gRNA
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available Since

   Oct. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LB101: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#2 gRNA

   Plasmid

   #131758

   Purpose

   pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   TRE#2 SaCas9 gRNA
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available Since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. phage - ubc - nls - 2xmcp - mCherry - BirA*

   Plasmid

   #131136

   Purpose

   BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and mCherry; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

   Depositor

   Ralf Jansen

   Insert

   NLS-HA-2xMCP-mCherry-BirA*
   

   Use

   Lentiviral

   Tags

   NLS, HA

   Expression

   Mammalian

   Mutation

   BirA* = BIrA >R118G

   Promoter

   human ubiquitin C (UBC)

   Available Since

   Aug. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. phage - ubc - nls - mcp - egfp - BirA*

   Plasmid

   #131134

   Purpose

   BirA* expressed as a protein fusion with MS2 coat protein (MCP) monomer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

   Depositor

   Ralf Jansen

   Insert

   NLS-HA-MCP-EGFP-BirA*
   

   Use

   Lentiviral

   Tags

   NLS, HA

   Expression

   Mammalian

   Mutation

   BirA* = BIrA >R118G

   Promoter

   human ubiquitin C (UBC)

   Available Since

   Aug. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. UAS-ras

   Plasmid

   #1825

   Depositor

   Philip Leder

   Insert

   c-HA-ras (HRAS Rat, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 4, 2005

   Availability

   Academic Institutions and Nonprofits only

9. phage - ubc - nls - 2xmcp - egfp - cMycBirA*

   Plasmid

   #131133

   Purpose

   BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

   Depositor

   Ralf Jansen

   Insert

   NLS-HA-2xMCP-EGFP-cMycBirA*
   

   Use

   Lentiviral

   Tags

   NLS, HA, cMyc (N-terminal of BirA*)

   Expression

   Mammalian

   Mutation

   BirA* = BIrA >R118G

   Promoter

   human ubiquitin C (UBC)

   Available Since

   Aug. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LB001: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#2 gRNA

    Plasmid

    #131757

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#2 Sp/xCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available Since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LA901: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#1 gRNA

    Plasmid

    #131756

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#1 Sp/xCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available Since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC57KAN-20XHA

    Plasmid

    #233114

    Purpose

    20X HA tag multimer

    Depositor

    Steve Stowers

    Insert

    HA tag
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC57KAN-5XHA

    Plasmid

    #233102

    Purpose

    5X HA tag multimer

    Depositor

    Steve Stowers

    Insert

    HA tag
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC57KAN-10XHA

    Plasmid

    #233108

    Purpose

    10X HA tag multimer

    Depositor

    Steve Stowers

    Insert

    HA tag
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBabe-puro Ras V12

    Plasmid

    #1768

    Depositor

    Bob Weinberg

    Insert

    ras V12 (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    G12V, constitutively active

    Promoter

    Available Since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBABE puro H-Ras V12

    Plasmid

    #9051

    Purpose

    Retroviral expression of a constitutively active form of H-Ras

    Depositor

    William Hahn

    Insert

    Hras V12 (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    G12V, constitutively active

    Promoter

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLentiN

    Plasmid

    #37444

    Purpose

    Control lentiviral plasmid containing FLAG-HA tags

    Depositor

    Karl Munger

    Article

    Spangle et al J Virol. 2012 May 2.

    Insert

    FLAG-HA
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pYLL201

    Plasmid

    #53454

    Purpose

    Expresses HA-tagged mouse DNA topoisomerase IIbeta (full length cDNA, in pCS2+ backbone)

    Depositor

    Lisa Lyu, Leroy Liu

    Article

    Ban et al Mol Cell Biol. 2013 Oct;33(20):4008-16.

    Insert

    HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) (Top2b Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-neo-cterminal-3HA

    Plasmid

    #102643

    Purpose

    To generate three copies of the HA epitope tag on cDNAs.

    Depositor

    Michael E. Wright

    Type

    Empty backbone

    Use

    Tags

    3x HA (YPYDVPDYA)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p5059

    Plasmid

    #198154

    Purpose

    bacterial expression of steC::HaloTag fusion

    Depositor

    Michael Hensel

    Insert

    PsteC steC::L16::HaloTag::HA
    

    Use

    Tags

    HaloTag-HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pYLL204

    Plasmid

    #53455

    Purpose

    Expresses active site tyrosine mutated HA-tagged mouse DNA topoisomerase IIbeta Y814F (full length cDNA, in pCS2+ backbone)

    Depositor

    Lisa Lyu, Leroy Liu

    Article

    Ban et al Mol Cell Biol. 2013 Oct;33(20):4008-16.

    Insert

    HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) Y814F (Top2b Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y814F

    Promoter

    CMV

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pCMV6-AN-HA_WT POLI

    Plasmid

    #201440

    Purpose

    Expresses human DNA polymerase iota with an N-terminal HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Coding sequence has been optimised for expression…

    Promoter

    CMV

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p3EHA

    Plasmid

    #67708

    Purpose

    3' entry HA tag

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    HA tag
    

    Use

    Tags

    HA

    Expression

    Mutation

    Promoter

    None

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p2643

    Plasmid

    #198145

    Purpose

    Epitope tagging of effector protein SseF

    Depositor

    Michael Hensel

    Insert

    PsseA::sscB sseF::HA (sseF Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAGGS_HA-VP35_EBOV

    Plasmid

    #103053

    Purpose

    Mammalian expression vector encoding HA-tagged Ebola virus VP35 gene under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al J Virol. 2016 Jul 27;90(16):7268-84. doi

    Insert

    VP35 (VP35 Zaire ebolavirus, Mayinga)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p4305

    Plasmid

    #198150

    Purpose

    bacterial expression of sifA::HaloTag fusion

    Depositor

    Michael Hensel

    Insert

    PsifA sifA::L16::HaloTag::HA (sifA Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    HaloTag-HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p4286

    Plasmid

    #198152

    Purpose

    bacterial expression of sseJ::HaloTag fusion

    Depositor

    Michael Hensel

    Insert

    PsseJ sseJ::L16::HaloTag::HA (sseJ Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    HaloTag-HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p4118

    Plasmid

    #198151

    Purpose

    bacterial expression of sseF::HaloTag fusion

    Depositor

    Michael Hensel

    Insert

    PsseA::sscB sseF::L16::HaloTag::HA (sseF Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    HaloTag-HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p4295

    Plasmid

    #198153

    Purpose

    bacterial expression of pipB2::HaloTag fusion

    Depositor

    Michael Hensel

    Insert

    PpipB2 pipB2::L16::HaloTag::HA (STM2780 Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    HaloTag-HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. YIplac211-yoHaloTag-TLG1

    Plasmid

    #105277

    Purpose

    Plasmid for pop-in/pop-out replacement of Tlg1 with yoHaloTag-Tlg1.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Tlg1 (TLG1 Budding Yeast)
    

    Use

    Tags

    yoHaloTag

    Expression

    Yeast

    Mutation

    HaloTag has yeast optimized codons

    Promoter

    Available Since

    Jan. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCVL SFFV-EF1s HA.NLS.Sce(opt)

    Plasmid

    #31479

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV/Ef1s HA NLS I-SceI
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Expression

    Mutation

    "opt" indicates the coding sequence has…

    Promoter

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. 2xMARS-nGFP

    Plasmid

    #205235

    Purpose

    Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

    Depositor

    Jeremy Baskin

    Insert

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    

    Use

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCDH-2xMARS-nGFP-puro

    Plasmid

    #205238

    Purpose

    Lentiviral vector encoding two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody

    Depositor

    Jeremy Baskin

    Insert

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    

    Use

    Lentiviral

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. MARS-nGFP

    Plasmid

    #205234

    Purpose

    Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

    Depositor

    Jeremy Baskin

    Insert

    PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody (PLEKHA5 Human)
    

    Use

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PPE18-ALFA

    Plasmid

    #231247

    Purpose

    Expresses a C-terminally tagged PPE18 (ALFA tag)

    Depositor

    Bryan Bryson

    Insert

    PPE18-ALFA (PPE18 Mycobacterium tuberculosis)
    

    Use

    Tags

    ALFA Tag

    Expression

    Bacterial

    Mutation

    Promoter

    hsp60

    Available Since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. p6173

    Plasmid

    #198157

    Purpose

    bacterial expression of sopD2::HaloTag fusion, mutant form

    Depositor

    Michael Hensel

    Insert

    PsopD2 sopD2 deltaCC::L16::HaloTag::HA (STM0972 Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    HaloTag-HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p6172

    Plasmid

    #198156

    Purpose

    bacterial expression of pipB2::HaloTag fusion, mutant form

    Depositor

    Michael Hensel

    Insert

    PpipB2 pipB2 deltaCC::L16::HaloTag::HA (STM2780 Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    HaloTag-HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. p6203

    Plasmid

    #198159

    Purpose

    bacterial expression of sseJ::HaloTag fusion, mutant form

    Depositor

    Michael Hensel

    Insert

    PsseJ sseJ deltaCC::L16::HaloTag::HA (sseJ Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    HaloTag-HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pENTR-R2L3-cHA-IRESpuro-pA

    Plasmid

    #139537

    Purpose

    Provides HA protein tag N-terminal to IRES-puro-pA cassette on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    HA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRDA174_EF1a_EnAsCas12a_BFP

    Plasmid

    #196724

    Purpose

    Sortable and selectable EnAsCas12a.

    Depositor

    Bernhard Schmierer

    Insert

    enASCas12a-HA-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS - HA and SV40 NLS

    Expression

    Mutation

    E174R, S542R, K548R

    Promoter

    EF1a

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge