We narrowed to 9,391 results for: Coli

1. pBEL2754

   Plasmid

   #195638

   Purpose

   Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; L245N)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   sfGFP-MlaA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   6xHis-TEV-sfGFP-MlaA (227-251; L245N)

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBEL2755

   Plasmid

   #195639

   Purpose

   Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I248N)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   sfGFP-MlaA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   6xHis-TEV-sfGFP-MlaA (227-251; I248N)

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBEL2756

   Plasmid

   #195640

   Purpose

   Expression/purification of 6xHis-TEV-sfGFP-MlaA(227-251; delta238-251)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   sfGFP-MlaA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   6xHis-TEV-sfGFP-MlaA(227-251; delta238-251)

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBEL2757

   Plasmid

   #195641

   Purpose

   Expression/purification of 6xHis-TEV-sfGFP-MlaA(227-251; delta244-251)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   sfGFP-MlaA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   6xHis-TEV-sfGFP-MlaA(227-251; delta244-251)

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBEL2766

   Plasmid

   #196028

   Purpose

   Complementation of MlaF-MlaE-MlaDdelta141-183-MlaC-MlaB

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   MlaFEDCB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   MlaF-MlaE-MlaDdelta141-183-MlaC-MlaB

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. psiCHECK-NP

   Plasmid

   #196607

   Purpose

   Dual-Luciferase Reporter vector carrying a 425bp-long Influenza virus NP sequence in forward direction

   Depositor

   Franco Lucchini

   Article

   Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

   Insert

   nucleocapsid protein (NP) gene, partial
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSF-p15A-T7- YFP

   Plasmid

   #186568

   Purpose

   p15A ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

   Depositor

   Teresa de Diego Puente

   Article

   Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

   Insert

   YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRSFDuet-1_mNeongreen-DARPinOFF7

   Plasmid

   #182414

   Purpose

   Bacterial expression of mNeongreen-DARPinOFF7 fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   mNeongreen-DARPinOFF7
   

   Use

   Tags

   mNeongreen

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRSFDuet-1_mNeongreen-Uricase

   Plasmid

   #182416

   Purpose

   Bacterial expression of mNeongreen-uricase fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   mNeongreen-uricase
   

   Use

   Tags

   mNeongreen

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSF-pMB1'- trc-YFP

    Plasmid

    #186565

    Purpose

    pMB1' ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

    Depositor

    Teresa de Diego Puente

    Article

    Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

    Insert

    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSF-pMB1'- tac-YFP

    Plasmid

    #186566

    Purpose

    pMB1' ori, lacI, Ptac promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

    Depositor

    Teresa de Diego Puente

    Article

    Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

    Insert

    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSCL.031

    Plasmid

    #184968

    Purpose

    Test effect of extending -Eco2 a1/a2 in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco2: RT and ncRNA(extended), a1/a2 length: 29
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    human codon optimized RT, a1/a2 length extended t…

    Promoter

    Gal7

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSCL.108

    Plasmid

    #184977

    Purpose

    Express -Eco1 LYP1 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 12
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    LYP1 donor E27stop

    Promoter

    Gal7

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSCL.106

    Plasmid

    #184975

    Purpose

    Express -Eco1 CAN1 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 12
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    CAN1 donor G444stop

    Promoter

    Gal7

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSCL.72

    Plasmid

    #184984

    Purpose

    Express Cas9/-Eco1 RT fusion, using linker1

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Cas9-fusion_linker1-Eco1RT
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSCL.95

    Plasmid

    #184986

    Purpose

    Express Cas9/-Eco1 RT fusion, using linker2

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Cas9-fusion_linker2-Eco1RT
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSLS.544

    Plasmid

    #184961

    Purpose

    Test effect of a1/a2 length on editing murF using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 22
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    murF donor G1359A, a1/a2 length extended to 22 bp

    Promoter

    T7/lac

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSCL.94

    Plasmid

    #184985

    Purpose

    Express -Eco1 RT/Cas9 fusion, using linker2

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1RT-fusion_linker2-Cas9
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSCL.037

    Plasmid

    #184966

    Purpose

    Express -Eco1 dead RT and ncRNA in yeasst

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: RT and ncRNA(wt), a1/a2 length: 12, dead RT
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Human codon optimized RT, YXDD catalytic region m…

    Promoter

    Gal7

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSCL.71

    Plasmid

    #184983

    Purpose

    Express -Eco1 RT/Cas9 fusion, using linker1

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1RT-fusion_linker1-Cas9
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSCL.112

    Plasmid

    #184981

    Purpose

    Express -Eco1 FAA1 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 12
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    FAA1 donor P233stop

    Promoter

    Gal7

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSCL.002

    Plasmid

    #184972

    Purpose

    Express -Eco1 ADE2 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 12
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ADE2 donor P272stop

    Promoter

    Gal7

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSCL.110

    Plasmid

    #184979

    Purpose

    Express -Eco1 TRP2 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 12
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    TRP2 donor E64stop

    Promoter

    Gal7

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRB116.2

    Plasmid

    #181946

    Purpose

    aTc-inducible expression of TorR with C-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    TorR-mNG; mCherry; tetR (AX04_RS23485, torR Synthetic, torR - E. coli)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial

    Mutation

    Promoter

    TorR-mNG-PLtetO-1; mCherry-PtorCAD129; tetR-J23106

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRB120.2

    Plasmid

    #181947

    Purpose

    aTc-inducible expression of TorR with N-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    mNG-TorR; mCherry; tetR (torR, AX04_RS23485 Synthetic, torR - E. coli)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial

    Mutation

    Promoter

    mNG-TorR-PLtetO-1; mCherry-PtorCAD129; tetR-J23106

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSLS.407

    Plasmid

    #187204

    Purpose

    Expresses retron Eco1 ncRNA v32, and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v32
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7/Lac

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSLS.408

    Plasmid

    #187205

    Purpose

    Expresses retron Eco1 ncRNA v35, and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v35
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7/Lac

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pNB_EA_3EGFP

    Plasmid

    #185967

    Purpose

    Expresses EGFP from PR promoter in E.coli Dh5alpha cell

    Depositor

    Sangram Bagh

    Article

    Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

    Insert

    Pr-Egfp cassette in forward direction in Network Brick
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PR

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28a-ammB3

    Plasmid

    #167815

    Purpose

    Expresses His6-tagged AmmB3 in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

    Insert

    AmmB3
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28a-ammC1

    Plasmid

    #167816

    Purpose

    Expresses His6-tagged AmmC1 in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

    Insert

    AmmC1
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pTS2341_Tier3(SB)-Puro

    Plasmid

    #169639

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFE-sfGFP

    Plasmid

    #162688

    Purpose

    pFE vector control expressing sfGFP

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    sfGFP
    

    Use

    Tags

    C terminal 6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHCKan-yibDp-CBD-hGLY

    Plasmid

    #134596

    Purpose

    Low Phosphate inducible hGLYAT2 with N-terminal chitin binding domain tag

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    human glycine acyltransferase 2 (GLYATL2 )
    

    Use

    Tags

    chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    yibD

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHCKan-yibDp-SBS-mCherry

    Plasmid

    #134598

    Purpose

    Low Phosphate inducible SBS (bonryl-diphosphate) synthase with C-terminal mCherry)

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    SBS-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHCKan-T7-CBD-hGLY

    Plasmid

    #134940

    Purpose

    T7 based expression of human glycine acyltransferase with an N-terminal chitin binding domain

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    human glycine acyltransferase 2 (GLYATL2 )
    

    Use

    Tags

    chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    t7

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJM143

    Plasmid

    #134166

    Purpose

    Retroviral plasmid expressing GFP - Nde1

    Depositor

    Iain Cheeseman

    Article

    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

    Insert

    Nde1_SSSC 1-220 E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    SSSC, E. coli codon optimized, CRISPR/Cas9 harden…

    Promoter

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. INPP5A / type I IP3 5-phosphatase from dog thyroid

    Plasmid

    #131767

    Purpose

    Expression in bacteria or COS-7 cells

    Depositor

    Christophe Erneux

    Article

    Verjans et al Biochem J. 1994 May 15;300 ( Pt 1):85-90

    Insert

    INPP5A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pNH-TrxT-G-T136D-S15

    Plasmid

    #127824

    Purpose

    Production of recombinant human ribosomal protein S15

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPS15 (RPS15 Human)
    

    Use

    Tags

    His6 - Thioredoxin - TEV

    Expression

    Bacterial

    Mutation

    T136D

    Promoter

    T7

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP

    Plasmid

    #124066

    Purpose

    E. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    cytATL-R232Q-sfGFP
    

    Use

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    R232Q

    Promoter

    trc

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAL800

    Plasmid

    #119306

    Purpose

    precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S

    Depositor

    Linda Randall

    Article

    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

    Insert

    precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    amino acids 1 through 171 deleted, and mutatioms …

    Promoter

    lac

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge