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pRDA_381

Plasmid

#179092

Purpose

nuclease Cas expression

Depositor

John Doench, David Root

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

IDT-Cas9_v2

Use

CRISPR and Lentiviral

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_157

Plasmid

#179090

Purpose

nuclease Cas expression

Depositor

John Doench, David Root

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

HypaCas9

Use

CRISPR and Lentiviral

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRDA_156

Plasmid

#179089

Purpose

nuclease Cas expression

Depositor

John Doench, David Root

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

Cas9-VRER

Use

CRISPR and Lentiviral

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRDA_155

Plasmid

#179088

Purpose

nuclease Cas expression

Depositor

John Doench, David Root

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

Cas9-VQR

Use

CRISPR and Lentiviral

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_154

Plasmid

#179087

Purpose

nuclease Cas expression

Depositor

John Doench, David Root

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

xCas9-3.7

Use

CRISPR and Lentiviral

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_153

Plasmid

#179086

Purpose

nuclease Cas expression

Depositor

John Doench, David Root

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

evoCas9

Use

CRISPR and Lentiviral

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRDA_152

Plasmid

#179085

Purpose

nuclease Cas expression

Depositor

John Doench, David Root

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

eCas9-1.1

Use

CRISPR and Lentiviral

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_151

Plasmid

#179084

Purpose

nuclease Cas expression

Depositor

John Doench, David Root

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

Cas9-HF

Use

CRISPR and Lentiviral

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
delta NT delta CT p70S6K1 MSCV puro

Plasmid

#8463

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

delta NT delta CT p70 S6K1 (Rps6kb1 Rat)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

p70 s6k1 form

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only
p6111 pSG5-HPV31-KWK-E8^E2C(HA)

Plasmid

#24742

Depositor

Peter Howley

Article

Powell et al J Virol. 2010 May;84(9):4451-60. doi: 10

Insert

HPV31 KWK E8^E2C (HA)

Tags

HA tag in hinge region

Expression

Mammalian

Mutation

residues 5 to 8 from KWK to AEA

Available Since

June 30, 2010

Availability

Academic Institutions and Nonprofits only
pMT-GFP-Pat coiled-coil (cc)

Plasmid

#31757

Depositor

Ron Vale

Article

Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

Insert

Patronin (Patronin Fly)

Tags

GFP

Expression

Insect

Mutation

Contains only the coiled-coil domain: aa 535-1457

Promoter

Metallothionein

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
SNAP-Paxillin

Plasmid

#186143

Purpose

For expresseion of recombinant paxillin in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PXN (PXN Human)

Tags

GST

Expression

Bacterial

Mutation

Paxillin C1997A (silent mutation for cloning)

Promoter

Lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV myc DAF-12

Plasmid

#32566

Depositor

Al Fisher

Article

Hochbaum et al PLoS Genet. 2011 Jul;7(7):e1002179. Epub

Insert

DAF-12 (daf-12 Nematode)

Tags

myc epitope

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only

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deltaCT S6K1 MSCV puro

Plasmid

#8465

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

delta ct p70 S6K1 (Rps6kb1 Rat)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

deletion of 104 c-terminal amino acids

Available Since

May 11, 2005

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmLSm2_A

Plasmid

#145881

Purpose

Insect Expression of DmLSm2

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm2 (CG10418 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmLSm3_A

Plasmid

#145882

Purpose

Insect Expression of DmLSm3

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm3 (LSm3 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmLSm5_A

Plasmid

#145884

Purpose

Insect Expression of DmLSm5

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm5 (CG6610 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmLSm7_A

Plasmid

#145885

Purpose

Insect Expression of DmLSm7

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm7 (LSm7 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
V179 pLP Triple-Flag - Acceptor

Plasmid

#11706

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

3xFLAG

Expression

Mammalian

Available Since

May 26, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFA1

Plasmid

#46789

Purpose

To express Fun30 in trans in budding yeast

Depositor

Patrick Varga-Weisz

Article

Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

Insert

FUN30 (FUN30 Budding Yeast)

Tags

V5

Expression

Yeast

Promoter

PGAL1

Available Since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only
iXFGFR1

Plasmid

#31257

Depositor

Masahiro Yamagishi

Article

Yamagishi et al Int J Dev Biol. 2010 . 54(1):93-104.

Insert

Xenopus FGFR1-ICD-Fv’Fv (fgfr1-a Frog)

Tags

HA epitope tag and myristoylation signal

Expression

Bacterial and Mammalian

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
pETx-SR6v15.d

Plasmid

#176213

Purpose

Bacterial expression of the SR6v15.d targeting the receptor binding domain of SARS-CoV-2 spike protein

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

SR6v15.d

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL3.7 Lmo4.2 shRNA

Plasmid

#59293

Purpose

shRNA to mouse Lmo4

Depositor

Ulrike Heberlein

Article

Lasek et al Genes Brain Behav. 2010 Oct;9(7):817-24.

Insert

Lmo4.2 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only
pLL3.7 Lmo4.3 shRNA

Plasmid

#59292

Purpose

shRNA to mouse Lmo4

Depositor

Ulrike Heberlein

Article

Lasek et al Genes Brain Behav. 2010 Oct;9(7):817-24.

Insert

Lmo4.3 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only
pJH807_pJH_LldR_Pcon-sfGFP.2TAG

Plasmid

#163738

Purpose

Constitutive expression of green fluorescent protein encoding two stop codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.2TAG

Expression

Bacterial

Mutation

gfp.N39TAG,Y135TAG

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH809_pJH_LldR_Pcon-sfGFP.10TAG

Plasmid

#163739

Purpose

Constitutive expression of green fluorescent protein encoding ten stop codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.10TAG

Expression

Bacterial

Mutation

gfp with first 10 leucine codons mutated to amber

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mCitrine-USP19 1-1290

Plasmid

#78595

Purpose

Express mCitrine-USP19 1-1290 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

USP19 1-1290 (USP19 Human)

Tags

mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only
ZE29 Stbm-deltaN-6myc-pCS2+

Plasmid

#17071

Depositor

Randall Moon

Article

Park et al Nat Cell Biol. 2002 Jan;4(1):20-5.

Insert

Stbm deltaN (vangl2 Zebrafish)

Use

Zebrafish expression

Tags

Myc6

Mutation

amino acids 240-526 only

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pETx-SR6v15.t

Plasmid

#176214

Purpose

Bacterial expression of the SR6v15.t targeting the receptor binding domain of SARS-CoV-2 spike protein

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

SR6v15.t

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PylT(CTAG)WT PylS mCherry-P2A-eGFP(CTAG)

Plasmid

#174527

Purpose

Dual-fluorescence reporter for quantifying PylT(CTAG)WT decoding efficiency

Depositor

Yu-Hsuan Tsai

Article

Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

Insert

PylS

Tags

Flag

Expression

Mammalian

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
V181 pLPS 3' Triple-Flag - Acceptor

Plasmid

#11708

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

3xFLAG

Expression

Mammalian

Available Since

May 26, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
V1579 pLP ProHis HTb - Acceptor

Plasmid

#11718

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

His

Expression

Bacterial

Available Since

June 2, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCAGGS/Daxx myc S502A

Plasmid

#19281

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

Daxx myc (Daxx Mouse)

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
S7_SwaI_Zeo_PacI

Plasmid

#128471

Purpose

Zeo Resistance gene

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

Zeocin Resistance gene

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AtFLS2pro::GUS

Plasmid

#87167

Purpose

expression pattern of AtFLS2

Depositor

Silke Robatzek

Article

Beck et al J Exp Bot. 2014 Dec;65(22):6487-98. doi:

Insert

AT5G46330 promoter

Tags

GUS

Promoter

AtFLS2

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBRIT Pax7 N281

Plasmid

#17524

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal 281 amino acids

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
S12_EcoRV_PGK_AscI

Plasmid

#128476

Purpose

PGK promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

PGK promoter

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR APLP1 shRNA 1

Plasmid

#30156

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al Neural Dev. 2008 Jun 23;3:15. doi: 10.11

Insert

APLP1 shRNA 1

Expression

Mammalian

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
p185 pK7-HDGF (GFP)

Plasmid

#11058

Depositor

Ramesh Shivdasani

Article

Tou et al Mol Cell Biol. 2004 Apr . 24(8):3132-9.

Insert

HDGF (Hdgf Rat)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
His10-PS-SNAPf-9E (XSB526)

Plasmid

#78514

Purpose

Construct for expressing His10 - SNAPf -9E in E. Coli

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

SNAPf

Tags

His10 followed by a prescission protease claevage…

Expression

Bacterial

Mutation

9E added to the C-terminal of SNAPf

Available Since

June 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVTH-shNP1

Plasmid

#65062

Purpose

Knockdown of NP1

Depositor

Ramon Trullas

Article

Figueiro-Silva et al J Neurosci. 2015 Apr 8;35(14):5504-21. d

Insert

shNP1 (Nptx1 Rat)

Use

Lentiviral

Expression

Mammalian

Promoter

H1

Available Since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS Tenomodulin

Plasmid

#19200

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Tenomodulin (TNMD Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pYPQ158

Plasmid

#69307

Purpose

Gateway entry vector with hSpCas9 (Human codon optimized)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

hSpCas9 (Human codon optimized)

Use

CRISPR

Tags

3XFLAG

Mutation

NLS fusion

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX4T3-NL2CT

Plasmid

#163623

Purpose

mouse NL2 N-terminally tagged with GST

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse NL2 (Nlgn2 Mouse)

Tags

GST

Expression

Mammalian

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-tWnt5a

Plasmid

#169372

Purpose

Expresses a part of Chinese soft-shell turtle Wnt5a for in situ hybridization probe.

Depositor

Katsuhiko Ono

Article

Nomura et al Cell Rep. 2020 May 5;31(5):107592. doi:

Insert

Wnt5a

Expression

Bacterial

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-tWnt7b

Plasmid

#169371

Purpose

Expresses a part of Chinese soft-shell turtle Wnt7b for in situ hybridization probe.

Depositor

Katsuhiko Ono

Article

Nomura et al Cell Rep. 2020 May 5;31(5):107592. doi:

Insert

Wnt7b

Expression

Bacterial

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS-ATG1(406)

Plasmid

#122068

Purpose

Expressing ATG1

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS Dynein light chain 1

Plasmid

#19213

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Dynein light chain 1

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 N391

Plasmid

#17525

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal 391 amino acids

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
ZE38 pCS2P+ sp5l ORF

Plasmid

#17079

Depositor

Randall Moon

Article

Thorpe et al Development. 2005 Apr. 132(8):1763-72.

Insert

sp5l ORF (sp5l Zebrafish)

Use

Zebrafish expression

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 5301 - 5350 of 22938 results

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We narrowed to 22,938 results for: Tes