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pBT378_pattB-pCA-GFP-pA-attB

Plasmid

#52554

Purpose

for PhiC31 integrase-mediated cassette exchange

Depositor

Liqun Luo

Publication

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLN380 (FuGW-S(CEBPB)p-mKate2)

Plasmid

#105178

Purpose

synthetic promoter CEBPB drives mKate2 expression

Depositor

Timothy Lu

Publication

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(CEBPB)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLN382 (FuGW-S(HIF1A)p-mKate2 )

Plasmid

#105180

Purpose

synthetic promoter HIF1A drives mKate2 expression

Depositor

Timothy Lu

Publication

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(HIF1A)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-evoCAST-eCas8

Plasmid

#234727

Purpose

Engineered human-codon optimized PseCas8 in mammalian cells (for evoCAST)

Depositor

David Liu

Publication

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

ePseCas8

Expression

Mammalian

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-evoCAST-TniQ

Plasmid

#234728

Purpose

Engineered human-codon optimized PseTniQ in mammalian cells (for evoCAST)

Depositor

David Liu

Publication

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

PseTniQ

Expression

Mammalian

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MBP-2xNLS-tdTomato

Plasmid

#104054

Purpose

An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

Depositor

Viviana Gradinaru

Publication

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

2xNLS-tdTomato

Use

AAV

Tags

NLS

Promoter

MBP

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMBP-MS2

Plasmid

#65104

Purpose

can be used to isolate RNA binding complexes

Depositor

Josep Vilardell

Publication

Macias et al Mol Cell. 2008 Jun 20;30(6):732-42. doi:

Insert

MS2

Tags

MBP

Expression

Bacterial

Mutation

double mutation (V75Q and A81G) that prevents oli…

Promoter

tac

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pTNT-B18R-6His

Plasmid

#58979

Purpose

expresses His-tagged B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

Depositor

Steven Dowdy

Publication

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

B18R

Tags

His

Expression

Bacterial

Promoter

T7/Sp6

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFP-parkin C431S

Plasmid

#45877

Depositor

Edward Fon

Publication

Trempe et al Science. 2013 May 9.

Insert

Parkin (PRKN Human)

Tags

EGFP

Expression

Mammalian

Mutation

C431S mutation (ubiquitin acceptor site)

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pQE-30-HyPer3

Plasmid

#42132

Purpose

Genetically encoded H2O2 probe for ratiometric and fluorescence lifetime imaging

Depositor

Vsevolod Belousov

Publication

Bilan et al ACS Chem Biol. 2012 Dec 20.

Insert

HYPER 3

Tags

6His

Expression

Bacterial

Promoter

T5

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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M-ST1cas

Plasmid

#48669

Purpose

Mammalian S. thermophilus #1 Cas9 expression, human optimized

Depositor

George Church

Publication

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9

Use

CRISPR

Tags

NLS

Promoter

CMV

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pEGFP-C1-FLAG-XRCC4

Plasmid

#46959

Purpose

Plasmid for expression of GFP-FLAG tagged human XRCC4 isoform 2 (N-terminal tag). Confers resistance to G418.

Depositor

Steve Jackson

Publication

Britton et al J Cell Biol. 2013 Jul 29.

Insert

XRCC4 (XRCC4 Human)

Tags

GFP-2xFLAG

Expression

Mammalian

Promoter

CMV

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pWJ66

Plasmid

#46570

Purpose

Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)

Depositor

Luciano Marraffini

Publication

Bikard et al Nucleic Acids Res. 2013 Jun 12.

Inserts

tracrRNA
dcas9-w
CRISPR array

Use

CRISPR

Tags

rpoZ

Expression

Bacterial

Mutation

D10A, H840A

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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3X FLAG-FUS-WT

Plasmid

#44985

Depositor

Robin Reed

Publication

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS (Full length) (FUS Human)

Tags

3X FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDONR223-MERTK

Plasmid

#23900

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MERTK (MERTK Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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CMV-HA-hTAF1

Plasmid

#17997

Depositor

Robert Tjian

Publication

Ruppert et al Nature. 1993 Mar 11. 362(6416):175-9.

Insert

hTAF1 (TAF1 Human)

Tags

HA

Expression

Mammalian

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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XE251 pCDNA3.1(zeo)-hDsh3

Plasmid

#16758

Depositor

Randall Moon

Publication

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

FLAG-hDsh3 (DVL3 Human)

Tags

flag

Expression

Mammalian

Available since

March 13, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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SARS-CoV2 Nucleocapsid

Plasmid

#153201

Purpose

Expresses myc-tagged nucleocapsid (N) from SARS-CoV2 in mammalian cells

Depositor

Peter Klein

Publication

Liu et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

Insert

Nucleocapsid from SARS-CoV-2 (N severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))

Tags

Myc tag (5X)

Expression

Mammalian

Promoter

CMV

Available since

July 15, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

5 Articles

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pTXB1-nbOcIgG-Tn5

Plasmid

#184285

Purpose

Anti-rabbit IgG Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

Depositor

New York Genome Center, Ivan Raimondi

Publication

Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

Insert

nbOcIgG-Tn5

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSR prdm3 (PRDM16 shRNA)

Plasmid

#15505

Depositor

Bruce Spiegelman

Publication

Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

Insert

PRDM16 shRNA (Prdm16 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles