We narrowed to 30,432 results for: Ide

1. (J) crtYB(K) G3 (SBE117)

   Plasmid

   #194996

   Purpose

   Rhodotorula toruloides gene phytoene synthase/lycopene cyclase (crtYB, RHTO_04605) for G3

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (J) crtYB(K)
   

   Expression

   Bacterial

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. (G) crtI (H) G2 (SBE116)

   Plasmid

   #194995

   Purpose

   Rhodotorula toruloides gene phytoene dehydrogenase (crtI, RHTO_04602) for G2

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (G) crtI (H)
   

   Expression

   Bacterial

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. (D) crtE (E)_G1 (SBE115)

   Plasmid

   #194994

   Purpose

   Rhodotorula toruloides gene geranylgeranyl diphosphate synthase (crtE, RHTO_02504) for G1

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (D) crtE (E)
   

   Expression

   Bacterial

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. (I) pGPD (J)_P3 (SBE111)

   Plasmid

   #194990

   Purpose

   promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P3 position (I/J)

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (I) pGPD (J)
   

   Expression

   Bacterial

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. (S) insD_ku70 (Q) (SBE139)

   Plasmid

   #187627

   Purpose

   the insertional region downstream for the deletion of the KU70 gene in R. toruloides. Overhangs S/Q

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (S) insD KU70 (Q)
   

   Expression

   Bacterial

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. (P) insUP_ku70 (SBE138) (B)

   Plasmid

   #187626

   Purpose

   the insertional region upstream for the deletion of the KU70 gene in R. toruloides. Overhangs P/B

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (P) insUP KU70 (B)
   

   Expression

   Bacterial

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. FgH1tUTG_muBim_Ex3

   Plasmid

   #85533

   Purpose

   Inducible expression of guide RNA (muBim_Ex3) with fluorescent GFP reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   mu Bim
   

   Promoter

   H1t

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. FgH1tUTG

   Plasmid

   #70183

   Purpose

   Inducible expression of guide RNA with fluorescent GFP reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   H1-Tet-sgrna cassette
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHS0812 hU6-BpiI-OgeuIscB omegaRNA scaffold

   Plasmid

   #176541

   Purpose

   human U6-driven expression of OgeuIscB omegaRNA scaffold with BpiI site for guide cloning

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   OgeuIscB omegaRNA scaffold
   

   Expression

   Mammalian

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC5Kan-P2A

    Plasmid

    #51814

    Purpose

    P2A peptide from porcine teschovirus-1 flanked by restriction sites in shuttle vector for easy cloning of multiple separate proteins to be expressed from single message

    Depositor

    Barry Ganetzky

    Article

    Daniels et al PLoS One. 2014 Jun 19;9(6):e100637. doi:

    Insert

    P2A peptide (PTV1gp1 porcine teschovirus-1, Synthetic)
    

    Use

    Shuttle vector for insect expression vectors

    Mutation

    codons altered for Drosophila and to eliminate re…

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. p8325 pLIX YAP1 WT

    Plasmid

    #184527

    Purpose

    Expression of YAP1 WT

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

    Insert

    YAP1 WT (YAP1 Human)
    

    Use

    Lentiviral

    Promoter

    TRE promoter, Tet ON

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. FgH1tUTG_mup53_Ex5

    Plasmid

    #85535

    Purpose

    Inducible expression of guide RNA (mup53_Ex5) with fluorescent GFP reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    mu p53
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    H1t

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-CRY2-5ptaseOCRL(D523G)

    Plasmid

    #66837

    Purpose

    Expression of inositol 5-phosphatase domain of OCRL(D523G - catalytically inactive mutant). Binds to CIBN upon blue light illumination.

    Depositor

    Pietro De Camilli

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    5ptaseOCRL (OCRL Human)
    

    Tags

    mCherry-CRY2

    Expression

    Mammalian

    Mutation

    D523G

    Promoter

    CMV

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-TGFα-GSY-SPM

    Plasmid

    #163615

    Purpose

    Expresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.

    Depositor

    Mark Howarth

    Article

    Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

    Insert

    TGFa-GSY-SPM (TGFA Human, Synthetic)
    

    Tags

    C-tag and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV Syn ChR E90R-D156N-T159C 2A tDimer

    Plasmid

    #52494

    Purpose

    Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

    Depositor

    Thomas Oertner

    Article

    Wietek et al Science. 2014 Mar 27.

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    E90R, D156N, T159C

    Promoter

    human synapsin-1 and ribosomal skip sequence

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. FgH1tUTG_huBim_Ex3

    Plasmid

    #85532

    Purpose

    Inducible expression of guide RNA (huBim_Ex3) with fluorescent GFP reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    hu Bim
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    H1t

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV Syn ChR E90R-T159C 2A tDimer

    Plasmid

    #52495

    Purpose

    Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

    Depositor

    Thomas Oertner

    Article

    Wietek et al Science. 2014 Mar 27.

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    E90R, T159C

    Promoter

    human synapsin-1 and ribosomal skip sequence

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. (B) HYG marker(R) (SBE264)

    Plasmid

    #195013

    Purpose

    hygromycin (HYG) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    pXYL+HYG+tGPD
    

    Expression

    Bacterial

    Promoter

    pXYL

    Available Since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. (B) BLE marker(R) (SBE262)

    Plasmid

    #195012

    Purpose

    bleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    pXYL+BLE+tGPD
    

    Expression

    Bacterial

    Promoter

    pXYL

    Available Since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. FgH1tUTG_huMcl-1.2

    Plasmid

    #85531

    Purpose

    Inducible expression of guide RNA (huMcl-1.2) with fluorescent GFP reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    hu Mcl-1.2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    H1t

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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