We narrowed to 2,603 results for: ubiquitin

1. pLV-SFFV-RAB10 Q68L-WPRE-UbC-Emerald

   Plasmid

   #203803

   Purpose

   Lentiviral vector plasmid expressing human RAB10 mutation Q68L (constitutively active mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Inserts

   RAB10 (RAB10 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Q68L

   Promoter

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. p667-UBC-CDK11B-V5-miniTurbo_IDG-K

   Plasmid

   #189914

   Purpose

   Gateway destination clone of CDK11B (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   CDK11B (CDK11B Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. px330-USP13

   Plasmid

   #61812

   Purpose

   to make USP13 Knockout cell line

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   USP13 (USP13 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pLV-SFFV-RAB10 T23N-WPRE-UbC-Emerald

   Plasmid

   #203802

   Purpose

   Lentiviral vector plasmid expressing human RAB10 mutation T23N (dominant negative mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Inserts

   RAB10 (RAB10 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   T23N

   Promoter

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-28a-His6-SUMO-VAMP2

   Plasmid

   #135551

   Purpose

   Express mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   VAMP2
   

   Use

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJOG022

   Plasmid

   #105384

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   promotor Zea mays Ubiquitin (1500bp)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI/ BpiI restriction sites removed

   Promoter

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCellFree_G03 ISG15

   Plasmid

   #67054

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   ISG15 (ISG15 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pET-28a-His6-SUMO-VAMP8

   Plasmid

   #135553

   Purpose

   Express mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   VAMP8
   

   Use

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTol1-Spe-KpnI-ubb-nmKate

   Plasmid

   #238373

   Purpose

   Empty backbone to generate guideRNA shuttles, that also drives ubiquitous expression of nuclear mKate.

   Depositor

   Juan Manuel González-Rosa

   Article

   Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

   Insert

   nmKate
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   ubiquitin

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVUT-eC3GFP

    Plasmid

    #225485

    Purpose

    Tet-on lentiviral vector for controlled expression of C. botulinum exoenzyme C3 transferase and EGFP

    Depositor

    Robert Gross

    Article

    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

    Insert

    Exoenzyme C3 transferase
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 30-251, See Depositor Comments

    Promoter

    Human ubiquitin C

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. L0-I16 tUBQ1 GG5-GG6

    Plasmid

    #195898

    Purpose

    Level 0 tUBQ1 terminator with GG5 GG6 golden gate linkers

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    tUBQ1 (UBQ1 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. L0-I15 tUBQ1 GG4-GG6

    Plasmid

    #195897

    Purpose

    Level 0 tUBQ1 terminator with GG4 GG6 golden gate linkers

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    tUBQ1 (UBQ1 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUiLMO2W

    Plasmid

    #129480

    Purpose

    3rd-generation lentiviral vector for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical

    Depositor

    Robert Gross

    Article

    Tung et al Sci Rep. 2015 Sep 24;5:14366. doi: 10.10

    Insert

    iLMO2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. OA-1052B (Ubiq-Fluc_Ubiq-Rluc)

    Plasmid

    #132426

    Purpose

    Express Firefly Luciferase-eGFP and Renilla Luciferase under Ubiquitin-63E promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Inserts

    Ubiq promoter
    firefly luciferase
    T2A-eGFP
    Renilla Luciferase-SV40-P10
    reversed ubiq promoter
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV.UBI.GFP.miR292T

    Plasmid

    #115972

    Purpose

    miR-292 regulated GFP expression

    Depositor

    Johan Jakobsson

    Article

    Sachdeva et al Proc Natl Acad Sci U S A. 2010 Jun 22;10

    Insert

    GFP.miR292T
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    ubiquitin

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPUbc (GB0145)

    Plasmid

    #68170

    Purpose

    Provides the S. Lycopersicum Ubiquitin carrier protein promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    SlUbc promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQStrep2-UBL3

    Plasmid

    #31330

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    ubiquitin-like 3 (UBL3 Human)
    

    Use

    Tags

    His, StrepII-tag, and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUbC-OsTIR1-myc-IRES-scFv-sfGFP

    Plasmid

    #84563

    Purpose

    Bicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFP

    Depositor

    Robert Singer

    Article

    Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

    Insert

    Transport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
    

    Use

    Lentiviral

    Tags

    myc tag (9x)

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-HDAC6.ΔBuz-FLAG

    Plasmid

    #30484

    Depositor

    Tso-Pang Yao

    Article

    Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

    Insert

    HDAC6 (HDAC6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deletion of the C-terminal Ubiquitin binding doma…

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFCIV-CEACAM6

    Plasmid

    #149303

    Purpose

    lentiviral packaging vector for CEACAM6 expression.

    Depositor

    James Fleckenstein

    Article

    Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

    Insert

    CEACAM6 (CEACAM6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ubiquitin C

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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